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Endocrinology Vol. 141, No. 6 2266-2274
Copyright © 2000 by The Endocrine Society

ARTICLES

Identification of an Estrogen-Mediated Deoxyribonucleic Acid-Binding Independent Transactivation Pathway on the Epidermal Growth Factor Receptor Gene Promoter1

Luisa Salvatori, Linda Ravenna, Maria Pia Felli, Maria Rosaria Cardillo, Matteo Antonio Russo, Luigi Frati, Alberto Gulino and Elisa Petrangeli

National Research Council (L.S., L.R., E.P.), Institute of Biomedical Technology, Rome; Department of Experimental Medicine (M.P.F.), University of L’Aquila, L’Aquila; Department of Experimental Medicine and Pathology (M.R.C., M.A.R., L.F., A.G.), University "La Sapienza", Rome; and Neuromed Institute (L.F., A.G.), Pozzilli, Italy

Address all correspondence and requests for reprints to: Dr. Elisa Petrangeli, Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, via G. B. Morgagni, 30/E, 00161 Roma, Italy. E-mail: petrang{at}itbm.rm.cnr.it

To investigate the estrogenic effects on the transcriptional regulation of the epidermal growth factor (EGF) receptor (EGFR) gene, we assayed its promoter ability to direct transcription of the luciferase reporter gene after transfection into HeLa cells. Our studies demonstrated a dose-dependent activation of the EGFR gene transcription by ligand-bound estrogen receptor {alpha} (ER{alpha}). This action was retained by the 36-bp core promoter fragment and did not require the receptor DNA binding domain, as demonstrated by analyzing the role of ER{alpha} deletion mutants on EGFR gene promoter-derived constructs. The 36-bp promoter fragment does not contain an estrogen response element but an imperfect thyroid hormone response element half-site that overlaps the Sp1 binding site. ER{alpha} does not bind this imperfect thyroid hormone response element half-site but is able to enhance binding of Sp1 to its site, in gel mobility shift assays, suggesting that the mechanism by which the receptor stimulated the transcription involved protein-protein interactions that replaced DNA binding. To explain this action, we propose a model in which induction of the EGFR gene expression by estrogens in HeLa cells is dependent upon the formation of a transcriptionally active ER{alpha}-Sp1 complex that binds to the GC-rich (Sp1) region of the minimal promoter.




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