This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Clausmeyer, S. Articles by Peters, J. Search for Related Content PubMed PubMed Citation Articles by Clausmeyer, S. Articles by Peters, J.

Tissue-Specific Expression of a Rat Renin Transcript Lacking the Coding Sequence for the Prefragment and Its Stimulation by Myocardial Infarction¹

Department of Pharmacology, University of Heidelberg (S.C., R.F., J.P.), and Deutsches Institut für Bluthochdruckforschung (T.U., S.C., J.P.), D-69120 Heidelberg, Germany; and Department of Pharmacology, University of Kiel (A.R., T.U.), 24105 Kiel, Germany

Address all correspondence and requests for reprints to: Dr. Susanne Clausmeyer, Department of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, D-69120 Heidelberg, Germany. E-mail: susanneclausmeyer{at}web.de

An alternative transcript of the rat renin gene was recently characterized in the adrenal gland, in addition to the known messenger RNA (mRNA) coding for preprorenin. In the alternative transcript, exon 1 is replaced by exon 1A, a domain originating in intron 1. The reading frame of this mRNA, termed exon 1A-renin transcript, codes for a truncated prorenin that presumably remains intracellular, in contrast to preprorenin, which is targeted to the secretory pathway by its prefragment. We here demonstrate the tissue-specific regulation of expression of both transcripts by RT and PCR. In many tissues both transcripts are present, for example in the adrenal gland, spleen, liver, and hypothalamus. In some organs, however, only one of the renin mRNAs is found. In the kidney only the full-length mRNA coding for preprorenin is detected. In the heart exclusively the exon 1A-mRNA is expressed, but not the preprorenin transcript. After myocardial infarction, which is known to activate the intracardiac renin-angiotensin system, expression of exon 1A-renin mRNA in the left ventricle was stimulated about 4-fold, compared with that in sham-operated animals, whereas no mRNA corresponding to preprorenin was detectable. These findings may have implications for the current concepts of local extrarenal renin-angiotensin systems, as they provide the molecular basis for a possible intracellular function of renin and exclude a role for locally produced secretory renin in the heart.

This article has been cited by other articles:

				V. P. Singh, B. Le, V. B. Bhat, K. M. Baker, and R. Kumar High-glucose-induced regulation of intracellular ANG II synthesis and nuclear redistribution in cardiac myocytes Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H939 - H948. [Abstract] [Full Text] [PDF]

				W. C. De Mello On the Pathophysiological Implications of an Intracellular Renin Receptor Circ. Res., December 8, 2006; 99(12): 1285 - 1286. [Full Text] [PDF]

				M. Paul, A. Poyan Mehr, and R. Kreutz Physiology of local Renin-Angiotensin systems. Physiol Rev, July 1, 2006; 86(3): 747 - 803. [Abstract] [Full Text] [PDF]

				J. L. Lavoie, X. Liu, R. A. Bianco, T. G. Beltz, A. K. Johnson, and C. D. Sigmund Evidence Supporting a Functional Role for Intracellular Renin in the Brain Hypertension, March 1, 2006; 47(3): 461 - 466. [Abstract] [Full Text] [PDF]

				W. C. De Mello and J. Monterrubio Intracellular and Extracellular Angiotensin II Enhance the L-Type Calcium Current in the Failing Heart Hypertension, September 1, 2004; 44(3): 360 - 364. [Abstract] [Full Text] [PDF]

				R. N. Re Intracellular Renin and the Nature of Intracrine Enzymes Hypertension, August 1, 2003; 42(2): 117 - 122. [Abstract] [Full Text] [PDF]

				R. N. Re Implications of intracrine hormone action for physiology and medicine Am J Physiol Heart Circ Physiol, March 1, 2003; 284(3): H751 - H757. [Full Text] [PDF]

				N. Seyedi, M. Koyama, C. J. Mackins, and R. Levi Ischemia Promotes Renin Activation and Angiotensin Formation in Sympathetic Nerve Terminals Isolated from the Human Heart: Contribution to Carrier-Mediated Norepinephrine Release J. Pharmacol. Exp. Ther., August 1, 2002; 302(2): 539 - 544. [Abstract] [Full Text] [PDF]

				J. Peters, R. Farrenkopf, S. Clausmeyer, J. Zimmer, S. Kantachuvesiri, M. G.F. Sharp, and J. J. Mullins Functional Significance of Prorenin Internalization in the Rat Heart Circ. Res., May 31, 2002; 90(10): 1135 - 1141. [Abstract] [Full Text] [PDF]

				M. Bader and D. Ganten It's Renin in the Brain: Transgenic Animals Elucidate the Brain Renin-Angiotensin System Circ. Res., January 11, 2002; 90(1): 8 - 10. [Full Text] [PDF]

				R. N. Re On Not Being the Last to Give Up the Old or the First to Adopt the New Hypertension, October 1, 2001; 38(4): 759 - 760. [Full Text] [PDF]