help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Arai, M.
Right arrow Articles by Abou-Samra, A. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arai, M.
Right arrow Articles by Abou-Samra, A. B.
Endocrinology Vol. 142, No. 1 446-454
Copyright © 2001 by The Endocrine Society

ARTICLES

Characterization of Three Corticotropin-Releasing Factor Receptors in Catfish: A Novel Third Receptor Is Predominantly Expressed in Pituitary and Urophysis1,2

Maya Arai, Iman Q. Assil and Abdul B. Abou-Samra

Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114

Address all correspondence and requests for reprints to: Dr. Abdul Abou-Samra, Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114. E-mail: samra{at}helix.mgh.harvard.edu

The present study reports the isolation of three complementary DNA (cDNA) clones encoding distinct subtypes of CRF receptors from the diploid catfish (cf) species, Ameiurus nebulosus. The first clone encodes a 446-amino acid protein (cfCRF-R1) that is highly homologous to mouse (m) CRF-R1 (93% identical). The cfCRF-R1 messenger RNA is highly expressed in the brain, and its distribution pattern correlates well with that of mammalian CRF-R1, except for weak expression in the pituitary. When transiently expressed in COS-7 cells, cfCRF-R1 bound CRF, urotensin I, and sauvagine with similar affinities. The second full-length cDNA, which was cloned from catfish heart, encodes a 406-amino acid protein that showed homology to murine CRF-R2 (88%) and when expressed in COS-7 cells preferentially bound sauvagine. The highest level of cfCRF-R2 expression was observed in the heart. The third full-length cDNA clone, which encodes a 428-amino acid protein, is structurally closer to cfCRF-R1 (85%) than to cfCRF-R2 (80%). This novel CRF receptor (cfCRF-R3) bound CRF with a 5-fold higher affinity than urotensin I and sauvagine and was expressed in the pituitary gland, urophysis, and brain. The presence of three different CRF receptors, each with distinct tissue distribution and ligand binding properties, suggests a complex CRF/urotensin I system.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. A. Zalutskaya, M. Arai, G. S. Bounoutas, and A. B. Abou-Samra
Impaired adaptation to repeated restraint and decreased response to cold in urocortin 1 knockout mice
Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E259 - E263.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
M. O Huising, L. M van der Aa, J. R Metz, A. de Fatima Mazon, B M L. V.-v. Kemenade, and G. Flik
Corticotropin-releasing factor (CRF) and CRF-binding protein expression in and release from the head kidney of common carp: evolutionary conservation of the adrenal CRF system
J. Endocrinol., June 1, 2007; 193(3): 349 - 357.
[Abstract] [Full Text] [PDF]

Home page
 Eur J EndocrinolHome page
J. A Majzoub
Corticotropin-releasing hormone physiology
Eur. J. Endocrinol., November 1, 2006; 155(suppl_1): S71 - S76.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. M. Craig, H. Al-Timimi, and N. J. Bernier
Differential Increase in Forebrain and Caudal Neurosecretory System Corticotropin-Releasing Factor and Urotensin I Gene Expression Associated with Seawater Transfer in Rainbow Trout
Endocrinology, September 1, 2005; 146(9): 3851 - 3860.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. H. Evans, P. M. Piermarini, and K. P. Choe
The Multifunctional Fish Gill: Dominant Site of Gas Exchange, Osmoregulation, Acid-Base Regulation, and Excretion of Nitrogenous Waste
Physiol Rev, January 1, 2005; 85(1): 97 - 177.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. De Groef, N. Goris, L. Arckens, E. R. Kuhn, and V. M. Darras
Corticotropin-Releasing Hormone (CRH)-Induced Thyrotropin Release Is Directly Mediated through CRH Receptor Type 2 on Thyrotropes
Endocrinology, December 1, 2003; 144(12): 5537 - 5544.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. H. Perrin, M. R. DiGruccio, S. C. Koerber, J. E. Rivier, K. S. Kunitake, D. L. Bain, W. H. Fischer, and W. W. Vale
A Soluble Form of the First Extracellular Domain of Mouse Type 2beta Corticotropin-releasing Factor Receptor Reveals Differential Ligand Specificity
J. Biol. Chem., April 25, 2003; 278(18): 15595 - 15600.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
R. L. Hauger, D. E. Grigoriadis, M. F. Dallman, P. M. Plotsky, W. W. Vale, and F. M. Dautzenberg
International Union of Pharmacology. XXXVI. Current Status of the Nomenclature for Receptors for Corticotropin-Releasing Factor and Their Ligands
Pharmacol. Rev., March 1, 2003; 55(1): 21 - 26.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Preil, M. B. Muller, A. Gesing, J. M. H. M. Reul, I. Sillaber, M. M. van Gaalen, J. Landgrebe, F. Holsboer, M. Stenzel-Poore, and W. Wurst
Regulation of the Hypothalamic-Pituitary-Adrenocortical System in Mice Deficient for CRH Receptors 1 and 2
Endocrinology, November 1, 2001; 142(11): 4946 - 4955.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
I. Q. Assil and A. B. Abou-Samra
N-glycosylation of CRF receptor type 1 is important for its ligand-specific interaction
Am J Physiol Endocrinol Metab, November 1, 2001; 281(5): E1015 - E1021.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. Wang, V. Martinez, J. E. Rivier, and Y. Tache
Peripheral urocortin inhibits gastric emptying and food intake in mice: differential role of CRF receptor 2
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2001; 281(5): R1401 - R1410.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A. SLOMINSKI, J. WORTSMAN, A. PISARCHIK, B. ZBYTEK, E. A. LINTON, J. E. MAZURKIEWICZ, and E. T. WEI
Cutaneous expression of corticotropin-releasing hormone (CRH), urocortin, and CRH receptors
FASEB J, August 1, 2001; 15(10): 1678 - 1693.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. H. Perrin, W. H. Fischer, K. S. Kunitake, A. G. Craig, S. C. Koerber, L. A. Cervini, J. E. Rivier, J. C. Groppe, J. Greenwald, S. M. Nielsen, et al.
Expression, Purification, and Characterization of a Soluble Form of the First Extracellular Domain of the Human Type 1 Corticotropin Releasing Factor Receptor
J. Biol. Chem., August 17, 2001; 276(34): 31528 - 31534.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2001 by The Endocrine Society