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Section on Endocrine Physiology, Developmental Endocrinology Branch, National Institute of Child Health and Human Development (H.A., G.A.); Laboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke (S.B.H., H.G.), NIH, Bethesda, Maryland 20892
Address all correspondence and requests for reprints to: Hiroshi Arima, Nagoya University School of Medicine, First Department of Internal Medicine, 65 Tsurumai-cho, Syowa, Nagoya 466-8550, Japan.
The regulation of arginine vasopressin (AVP) gene transcription in the paraventricular nucleus (PVN) was studied in rat hypothalamic organotypic cultures using intronic in situ hybridization. While AVP heteronuclear (hn) RNA was not detected in the PVN under basal conditions, a marked induction of AVP hnRNA was observed after 2 and 3 h incubation of slices with forskolin. In contrast to the stimulatory effects of forskolin, phorbol 12-myristate 13-acetate (PMA) was completely ineffective in inducing AVP hnRNA in the PVN at any time examined (1–3 h). Forskolin-induced AVP hnRNA expression was unaffected by blockage of neurotransmission by the sodium channel inhibitor, tetrodotoxin, indicating that forskolin acts directly on AVP cells in the PVN. Dual staining in situ hybridization of forskolin-stimulated hypothalamic sections using both radio labeled AVP hnRNA and digoxigenin-labeled CRH mRNA probes revealed colocalization of both transcripts, indicating AVP hnRNA is expressed in the parvocellular neurons. The data demonstrate that cAMP directly activates AVP gene transcription in parvocellular neurons of the PVN.
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