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Endocrinology Vol. 142, No. 2 645-652
Copyright © 2001 by The Endocrine Society

ARTICLES

Regulation of Metallothionein II Messenger Ribonucleic Acid Measures Exogenous Estrogen Receptor-ß Activity in SAOS-2 and LNCaPLN3 Cells

Heather A. Harris, Ruth A. Henderson, Ramesh A. Bhat and Barry S. Komm

Women’s Health Research Institute, Wyeth-Ayerst Research, Radnor, Pennsylvania 19087

Address all correspondence and requests for reprints to: Dr. Heather A. Harris, Women’s Health Research Institute, Wyeth-Ayerst Research, 145 King of Prussia Road, Radnor, Pennsylvania 19087. E-mail: harrish{at}war.wyeth.com

Estrogen receptor-ß (ERß) is a recently discovered member of the steroid hormone superfamily. Because its distribution is distinct from that of the classical estrogen receptor, and it is expressed in several nonclassical estrogen target tissues (e.g. prostate and bladder), its role in mediating the action of estrogen is unclear. One approach to elucidating the function of this receptor is to identify genes that it regulates. Using differential display, we profiled the messenger RNAs regulated by 17ß-estradiol in SAOS-2 and LNCaPLN3 cells overexpressing ERß. Follow-up studies used cells expressing either ER{alpha} or ERß. One gene, metallothionein II, was regulated by both receptor subtypes in LNCaPLN3 cells, but only by ERß in SAOS-2 cells. Because cycloheximide blocks this response, induction is probably mediated through regulation of at least one other protein. Identification of endogenous genes that are regulated differentially by ER{alpha} and ERß will be valuable tools in elucidating the function of ERß and the mechanisms by which these two receptors regulate transcription.




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Copyright © 2001 by The Endocrine Society