help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Prigent-Tessier, A.
Right arrow Articles by Gibori, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prigent-Tessier, A.
Right arrow Articles by Gibori, G.
Endocrinology Vol. 142, No. 3 1242-1250
Copyright © 2001 by The Endocrine Society

ARTICLES

Characterization of a Rat Uterine Cell Line, UIII Cells: Prolactin (PRL) Expression and Endogenous Regulation of PRL-Dependent Genes; Estrogen Receptor {beta}, {alpha}2-Macroglobulin, and Decidual PRL Involving the Jak2 and Stat5 Pathway1

Anne Prigent-Tessier2, Uriel Barkai2, Christian Tessier, Hélène Cohen and Geula Gibori

Department of Physiology and Biophysics (A.P.-T., U.B., C.T., G.G.), College of Medicine, University of Illinois, Chicago, Illinois 60612; Institut National des Sciences Appliquées de Lyon-INSERM U352 (H.C.), Laboratoire de Biochimie et Pharmacologie, 69100 Villeurbanne Cedex, France

Address all correspondence and requests for reprints to: Dr. Geula Gibori, Department of Physiology and Biophysics (M/C 901), University of Illinois, 835 South Wolcott Avenue, Chicago, Illinois 60612-7342. E-mail: ggibori{at}uic.edu

Decidualization of endometrial stroma in the rat induces the expression and secretion of rat decidual PRL (rdPRL). Recently, we have generated a nontransformed rat uterine stromal cell line (UIII) that decidualizes spontaneously in culture. In this report, we have established by immunocytochemistry, RT-PCR, Western blot analysis, labeled amino acid incorporation and RIA that these cells express the rat PRL messenger RNA as well as synthesize and secrete PRL. We have also cloned by RT-PCR a 403-bp complementary DNA fragment whose sequence is identical with that of rat pituitary PRL. In addition, UIII cells express the PRL receptor (PRL-R) long form, all the components involved in the PRL signal transduction pathway, estrogen receptor {beta} (ER{beta}) and {alpha}2-macroglobulin ({alpha}2-MG), which are known to be PRL-regulated genes. However, when UIII cells were treated with PRL, no regulation of these genes was observed. Moreover, in these cells, the PRL signaling components: the tyrosine kinase Jak2 and the transcription factor Stat5 were endogenously phosphorylated and their phosphorylation states were not enhanced in the presence of exogenous PRL. To examine whether the endogenously secreted PRL affects the expression of PRL-regulated genes, UIII cells were treated with either an anti-PRL receptor antibody or a Jak2 inhibitor, AG490. The anti-PRL receptor antibody decreased {alpha}2-MG expression. AG490 inhibited Jak2 and Stat5 phosphorylation, prevented Stat5 binding to its DNA consensus sequence, and also caused a dose-dependent down-regulation of {alpha}2-MG and ER{beta} expression. In contrast, AG490 enhanced PRL mRNA levels. In summary, we have established that the UIII stromal cells of uterine origin produce PRL. Furthermore, we have shown for the first time that decidual PRL may act locally to activate the Jak2/Stat5 pathway and up-regulate important genes involved in decidual growth and placentation.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
Y. S. Devi, A. Shehu, C. Stocco, J. Halperin, J. Le, A. M. Seibold, M. Lahav, N. Binart, and G. Gibori
Regulation of Transcription Factors and Repression of Sp1 by Prolactin Signaling Through the Short Isoform of Its Cognate Receptor
Endocrinology, July 1, 2009; 150(7): 3327 - 3335.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Nagashima, T. Maruyama, H. Uchida, T. Kajitani, T. Arase, M. Ono, H. Oda, M. Kagami, H. Masuda, S. Nishikawa, et al.
Activation of SRC Kinase and Phosphorylation of Signal Transducer and Activator of Transcription-5 Are Required for Decidual Transformation of Human Endometrial Stromal Cells
Endocrinology, March 1, 2008; 149(3): 1227 - 1234.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. Halperin, S. Y. Devi, S. Elizur, C. Stocco, A. Shehu, D. Rebourcet, T. G. Unterman, N. D. Leslie, J. Le, N. Binart, et al.
Prolactin Signaling through the Short Form of Its Receptor Represses Forkhead Transcription Factor FOXO3 and Its Target Gene Galt Causing a Severe Ovarian Defect
Mol. Endocrinol., February 1, 2008; 22(2): 513 - 522.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. Li, Y. S. Devi, L. Bao, J. Mao, and G. Gibori
Involvement of Cyclin D3, CDKN1A (p21), and BIRC5 (Survivin) in Interleukin 11 Stimulation of Decidualization in Mice
Biol Reprod, January 1, 2008; 78(1): 127 - 133.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Bao, C. Tessier, A. Prigent-Tessier, F. Li, O. L. Buzzio, E. A. Callegari, N. D. Horseman, and G. Gibori
Decidual Prolactin Silences the Expression of Genes Detrimental to Pregnancy
Endocrinology, May 1, 2007; 148(5): 2326 - 2334.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
L. Bao, S. Devi, J. Bowen-Shauver, S. Ferguson-Gottschall, L. Robb, and G. Gibori
The Role of Interleukin-11 in Pregnancy Involves Up-Regulation of {alpha}2-Macroglobulin Gene through Janus Kinase 2-Signal Transducer and Activator of Transcription 3 Pathway in the Decidua
Mol. Endocrinol., December 1, 2006; 20(12): 3240 - 3250.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
F. Y. Ma, G. M. Anderson, T. D. Gunn, V. Goffin, D. R. Grattan, and S. J. Bunn
Prolactin Specifically Activates Signal Transducer and Activator of Transcription 5b in Neuroendocrine Dopaminergic Neurons
Endocrinology, December 1, 2005; 146(12): 5112 - 5119.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C.M. Perks, P.V. Newcomb, M. Grohmann, R.J. Wright, H.D. Mason, and J.M.P. Holly
Prolactin acts as a potent survival factor against C2-ceramide-induced apoptosis in human granulosa cells
Hum. Reprod., December 1, 2003; 18(12): 2672 - 2677.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. Tessier, A. Prigent-Tessier, L. Bao, C. M. Telleria, S. Ferguson-Gottschall, G. B. Gibori, Y. Gu, J. M. Bowen-Shauver, N. D. Horseman, and G. Gibori
Decidual Activin: Its Role In the Apoptotic Process and Its Regulation by Prolactin
Biol Reprod, May 1, 2003; 68(5): 1687 - 1694.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
N. Baran, P. A. Kelly, and N. Binart
Decysin, a New Member of the Metalloproteinase Family, Is Regulated by Prolactin and Steroids During Mouse Pregnancy
Biol Reprod, May 1, 2003; 68(5): 1787 - 1792.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
X. Li, A. Warri, S. Makela, T. Ahonen, T. Streng, R. Santti, and M. Poutanen
Mammary Gland Development in Transgenic Male Mice Expressing Human P450 Aromatase
Endocrinology, October 1, 2002; 143(10): 4074 - 4083.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Tessier, A. Prigent-Tessier, S. Ferguson-Gottschall, Y. Gu, and G. Gibori
PRL Antiapoptotic Effect in the Rat Decidua Involves the PI3K/Protein Kinase B-Mediated Inhibition of Caspase-3 Activity
Endocrinology, September 1, 2001; 142(9): 4086 - 4094.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2001 by The Endocrine Society