| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
ARTICLES |
Program in Neuroscience and Department of Psychology, Stanford University, Stanford, California 94305-2130; Department of Biochemistry, University of Cape Town (N.I., M.M., R.P.M.), Rondesbosch 7700, South Africa; and Medical Research Council Research Unit for Molecular Reproductive Endocrinology, University of Cape Town Medical School (B.E.T.), Cape Town, South Africa
Address all correspondence and requests for reprints to: Dr. R. D. Fernald, Program in Neuroscience and Department of Psychology, Stanford University, Stanford, California 94305-2130. E-mail: russ{at}psych.stanford.edu
GnRH acts via GnRH receptors (GnRH-R) in the pituitary to cause the release of gonadotropins that regulate vertebrate reproduction. In the teleost fish, Haplochromis burtoni, reproduction is socially regulated through the hypothalamus-pituitary-gonadal axis, making the pituitary GnRH-R a likely site of action for this control. As a first step toward understanding the role of GnRH-R in the social control of reproduction, we cloned and sequenced candidate GnRH-R complementary DNAs from H. burtoni tissue. We isolated a complementary DNA that predicts a peptide encoding a G protein-coupled receptor that shows highest overall identity to other fish type I GnRH-R (goldfish IA and IB and African catfish). Functional testing of the expressed protein in vitro confirmed high affinity binding of multiple forms of GnRH. Localization of GnRH-R messenger RNA using RT-PCR revealed that it is widely distributed in the brain and retina as well as elsewhere in the body. Taken together, these data suggest that this H. burtoni GnRH receptor probably interacts in vivo with all three forms of GnRH.
This article has been cited by other articles:
 |
|
 |
|
  C. A. Flanagan, C.-C. Chen, M. Coetsee, S. Mamputha, K. E. Whitlock, N. Bredenkamp, L. Grosenick, R. D. Fernald, and N. Illing Expression, Structure, Function, and Evolution of Gonadotropin-Releasing Hormone (GnRH) Receptors GnRH-R1SHS and GnRH-R2PEY in the Teleost, Astatotilapia burtoni Endocrinology, October 1, 2007; 148(10): 5060 - 5071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Silver, N. V. Nucci, A. R. Root, K. L. Reed, and S. A. Sower Cloning and Characterization of a Functional Type II Gonadotropin-Releasing Hormone Receptor with a Lengthy Carboxy-Terminal Tail from an Ancestral Vertebrate, the Sea Lamprey Endocrinology, August 1, 2005; 146(8): 3351 - 3361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Levavi-Sivan, H. Safarian, H. Rosenfeld, A. Elizur, and A. Avitan Regulation of Gonadotropin-Releasing Hormone (GnRH)-Receptor Gene Expression in Tilapia: Effect of GnRH and Dopamine Biol Reprod, June 1, 2004; 70(6): 1545 - 1551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gonzalez-Martinez, T. Madigou, E. Mananos, J. M. Cerda-Reverter, S. Zanuy, O. Kah, and J. A. Munoz-Cueto Cloning and Expression of Gonadotropin-Releasing Hormone Receptor in the Brain and Pituitary of the European Sea Bass: An In Situ Hybridization Study Biol Reprod, May 1, 2004; 70(5): 1380 - 1391. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Millar, Z.-L. Lu, A. J. Pawson, C. A. Flanagan, K. Morgan, and S. R. Maudsley Gonadotropin-Releasing Hormone Receptors Endocr. Rev., April 1, 2004; 25(2): 235 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Fernald How does Behavior Change the Brain? Multiple Methods to Answer Old Questions Integr. Comp. Biol., December 1, 2003; 43(6): 771 - 779. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
