help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Asselin, E.
Right arrow Articles by Tsang, B. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Asselin, E.
Right arrow Articles by Tsang, B. K.
Endocrinology Vol. 142, No. 6 2451-2457
Copyright © 2001 by The Endocrine Society

ARTICLES

X-Linked Inhibitor of Apoptosis Protein Activates the Phosphatidylinositol 3-Kinase/Akt Pathway in Rat Granulosa Cells during Follicular Development1

Eric Asselin2, Yifang Wang and Benjamin K. Tsang

Reproductive Biology Unit and Division of Reproductive Medicine, Departments of Obstetrics and Gynecology and Cellular and Molecular Medicine, University of Ottawa; and Ottawa Health Research Institute, The Ottawa Hospital (Civic Campus), Ottawa, Ontario, Canada K1Y 4E9

Address all correspondence and requests for reprints to: Benjamin K. Tsang, Ph.D., Ottawa Health Research Institute, The Ottawa Hospital (Civic Campus), 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9. E-mail: btsang{at}lri.ca

X-linked inhibitor of apoptosis protein (XIAP) in granulosa cells is regulated by gonadotropins during follicular development, although the current understanding of the mechanisms by which XIAP suppressed granulosa cell apoptosis is incomplete. In the present study, we investigated the possible involvement of the phosphatidylinositol 3-kinase (PI 3-K) survival pathway in the regulation of granulosa cell fate. Using a fully characterized in vivo model to study the induction of follicular development and atresia in immature rats, we have demonstrated that gonadotropin treatment increased granulosa cell XIAP and phospho-Akt protein contents and suppressed apoptosis. In addition, gonadotropin withdrawal [equine CG (eCG)-primed rats treated with an anti-eCG antibody] induced granulosa cell apoptosis and significantly decreased ovarian weight. The increased apoptosis was accompanied by marked decreases in XIAP expression and phosphorylation of Akt protein. Infection of granulosa cells from eCG-primed rats with adenoviral sense XIAP [lacZ as a control; multiplicity of infection, 1–5] resulted in XIAP overexpression and increased phospho-Akt content, whereas XIAP antisense expression (multiplicity of infection, 10–40) decreased granulosa cell phospho-Akt level and induced apoptosis. Addition of the specific PI 3-K inhibitor LY294002 to the granulosa cell cultures decreased Akt phosphorylation and induced apoptosis in a dose-dependent manner. Taken together, these results demonstrate for the first time the importance and regulation of the PI 3-K survival pathway by XIAP in the control granulosa cell apoptosis.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
C. Van Themsche, V. Leblanc, S. Parent, and E. Asselin
X-linked Inhibitor of Apoptosis Protein (XIAP) Regulates PTEN Ubiquitination, Content, and Compartmentalization
J. Biol. Chem., July 31, 2009; 284(31): 20462 - 20466.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
P. Chaudhry and E. Asselin
Resistance to chemotherapy and hormone therapy in endometrial cancer
Endocr. Relat. Cancer, June 1, 2009; 16(2): 363 - 380.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
K. M. Aird, X. Ding, A. Baras, J. Wei, M. A. Morse, T. Clay, H. K. Lyerly, and G. R. Devi
Trastuzumab signaling in ErbB2-overexpressing inflammatory breast cancer correlates with X-linked inhibitor of apoptosis protein expression
Mol. Cancer Ther., January 1, 2008; 7(1): 38 - 47.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
H. Wang and B. K Tsang
Nodal signalling and apoptosis
Reproduction, May 1, 2007; 133(5): 847 - 853.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
H. Wang, J.-Y. Jiang, C. Zhu, C. Peng, and B. K. Tsang
Role and Regulation of Nodal/Activin Receptor-Like Kinase 7 Signaling Pathway in the Control of Ovarian Follicular Atresia
Mol. Endocrinol., October 1, 2006; 20(10): 2469 - 2482.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. Orisaka, S. Orisaka, J.-Y. Jiang, J. Craig, Y. Wang, F. Kotsuji, and B. K. Tsang
Growth Differentiation Factor 9 Is Antiapoptotic during Follicular Development from Preantral to Early Antral Stage
Mol. Endocrinol., October 1, 2006; 20(10): 2456 - 2468.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
R. Z. Spaczynski, J. L. Tilly, A. Mansour, and A. J. Duleba
Insulin and insulin-like growth factors inhibit and luteinizing hormone augments ovarian theca-interstitial cell apoptosis
Mol. Hum. Reprod., May 1, 2005; 11(5): 319 - 324.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
M. R. Hussein
Apoptosis in the ovary: molecular mechanisms
Hum. Reprod. Update, March 1, 2005; 11(2): 162 - 178.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
W. E. Thompson, E. Asselin, A. Branch, J. K. Stiles, P. Sutovsky, L. Lai, G.-S. Im, R. S. Prather, S. C. Isom, E. Rucker III, et al.
Regulation of Prohibitin Expression During Follicular Development and Atresia in the Mammalian Ovary
Biol Reprod, July 1, 2004; 71(1): 282 - 290.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
A. Amantana, C. A. London, P. L. Iversen, and G. R. Devi
X-linked inhibitor of apoptosis protein inhibition induces apoptosis and enhances chemotherapy sensitivity in human prostate cancer cells
Mol. Cancer Ther., June 1, 2004; 3(6): 699 - 707.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. R. Lareu, M. D. Lacher, C. K. Bradley, R. Sridaran, R. R. Friis, and A. M. Dharmarajan
Regulated Expression of Inhibitor of Apoptosis Protein 3 in the Rat Corpus Luteum
Biol Reprod, June 1, 2003; 68(6): 2232 - 2240.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Y. Wang, P. U. Rippstein, and B. K. Tsang
Role and Gonadotrophic Regulation of X-Linked Inhibitor of Apoptosis Protein Expression During Rat Ovarian Follicular Development In Vitro
Biol Reprod, February 1, 2003; 68(2): 610 - 619.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. Wu Xiao, E. Asselin, and B. K. Tsang
Nuclear Factor {kappa}B-Mediated Induction of Flice-Like Inhibitory Protein Prevents Tumor Necrosis Factor {alpha}-Induced Apoptosis in Rat Granulosa Cells
Biol Reprod, August 1, 2002; 67(2): 436 - 441.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y. Wang, S. Chan, and B. K. Tsang
Involvement of Inhibitory Nuclear Factor-{kappa}B (NF{kappa}B)-Independent NF{kappa}B Activation in the Gonadotropic Regulation of X-Linked Inhibitor of Apoptosis Expression during Ovarian Follicular Development in Vitro
Endocrinology, July 1, 2002; 143(7): 2732 - 2740.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Y. Wang, E. Asselin, and B. K. Tsang
Involvement of Transforming Growth Factor {alpha} in the Regulation of Rat Ovarian X-Linked Inhibitor of Apoptosis Protein Expression and Follicular Growth by Follicle-Stimulating Hormone
Biol Reprod, June 1, 2002; 66(6): 1672 - 1680.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2001 by The Endocrine Society