| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
ARTICLES |
Department of Pathology (K.H.B., C.Y., T.R.K., M.M.M.), Department of Molecular and Human Genetics (K.H.B., M.M.M.), and Department of Molecular and Cellular Biology (T.R.K., M.M.M.), Baylor College of Medicine, Houston, Texas 77030
Address all correspondence and requests for reprints to: Martin M. Matzuk, M.D., Ph.D., Professor and Stuart A. Wallace Chair, Department of Pathology, One Baylor Plaza, Baylor College of Medicine, Houston Texas 77030.
FSH is a heterodimeric glycoprotein hormone that is produced in the
gonadotroph cells of the anterior pituitary. It acts on Sertoli cells
of the testis and granulosa cells of the ovary. We previously
demonstrated that FSHß knockout female mice are infertile due to a
block in folliculogenesis preceding antral stage development. To
investigate aberrations of ovarian gene regulation in the absence of
FSH, we analyzed the expression of several important marker genes using
Northern blot and in situ hybridization techniques. Key
findings are as follows: 1) Follicles of FSHß knockout mice develop a
well organized thecal layer, which is positive for P450
17
-hydroxylase and LH receptor messenger RNAs (mRNAs). This
indicates that theca recruitment is completed autonomously with respect
to FSH. 2) Granulosa cells in FSH-deficient mice demonstrate an
increase in FSH receptor mRNA, and decreases in P450 aromatase,
serum/glucocorticoid-induced kinase, and inhibin/activin subunit
mRNAs. These data support studies that implicate FSH signaling cascades
in the expression of these genes. 3) In contrast to the thecal layer,
granulosa cell populations in FSHß knockout mice do not accumulate LH
receptor mRNA. This suggests that although the granulosa cells have a
block in proliferation at the antral follicle stage in the absence of
FSH, they do not initiate programs of terminal differentiation as seen
in luteinizing cells of wild-type ovaries. 4) Ovaries of FSH-deficient
mice demonstrate a modest decrease in cyclin D2 mRNA, without
up-regulation of cell cycle inhibitor mRNAs associated with
luteinization (i.e. p15, p27, and p21). Although
components of the FSH null phenotype may be caused by partial cyclin D2
loss of function, these findings indicate that the mechanisms of
granulosa cell cycle arrest in FSHß knockout mice are distinct from
those of cycle withdrawal at luteinization. Underscoring the usefulness
of the FSH-deficient mouse model, this study clarifies aspects of
gonadotropin-dependent folliculogenesis, thecal layer development,
cycle control in granulosa cells, and luteinization.
This article has been cited by other articles:
 |
|
 |
|
  B. D. Looyenga and G. D. Hammer Genetic Removal of Smad3 from Inhibin-Null Mice Attenuates Tumor Progression by Uncoupling Extracellular Mitogenic Signals from the Cell Cycle Machinery Mol. Endocrinol., October 1, 2007; 21(10): 2440 - 2457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Tiwari-Pandey, Y. Yang, J. Aravindakshan, and M.R. Sairam Normalization of hormonal imbalances, ovarian follicular dynamics and metabolic effects in follitrophin receptor knockout mice Mol. Hum. Reprod., May 1, 2007; 13(5): 287 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.R. Barnett, C. Schilling, C.R. Greenfeld, D. Tomic, and J.A. Flaws Ovarian follicle development and transgenic mouse models Hum. Reprod. Update, September 1, 2006; 12(5): 537 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Luo and M. C. Wiltbank Distinct Regulation by Steroids of Messenger RNAs for FSHR and CYP19A1 in Bovine Granulosa Cells Biol Reprod, August 1, 2006; 75(2): 217 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Pangas, X. Li, E. J. Robertson, and M. M. Matzuk Premature Luteinization and Cumulus Cell Defects in Ovarian-Specific Smad4 Knockout Mice Mol. Endocrinol., June 1, 2006; 20(6): 1406 - 1422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jablonka-Shariff, T. R. Kumar, J. Eklund, A. Comstock, and I. Boime Single-Chain, Triple-Domain Gonadotropin Analogs with Disulfide Bond Mutations in the {alpha}-Subunit Elicit Dual Follitropin and Lutropin Activities in Vivo Mol. Endocrinol., June 1, 2006; 20(6): 1437 - 1446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shiina, T. Matsumoto, T. Sato, K. Igarashi, J. Miyamoto, S. Takemasa, M. Sakari, I. Takada, T. Nakamura, D. Metzger, et al. From the Cover: Premature ovarian failure in androgen receptor-deficient mice PNAS, January 3, 2006; 103(1): 224 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. T. Ruiz-Cortes, S. Kimmins, L. Monaco, K. H. Burns, P. Sassone-Corsi, and B. D. Murphy Estrogen Mediates Phosphorylation of Histone H3 in Ovarian Follicle and Mammary Epithelial Tumor Cells via the Mitotic Kinase, Aurora B Mol. Endocrinol., December 1, 2005; 19(12): 2991 - 3000. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T R. Kumar What have we learned about gonadotropin function from gonadotropin subunit and receptor knockout mice? Reproduction, September 1, 2005; 130(3): 293 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Couse, M. M. Yates, B. J. Deroo, and K. S. Korach Estrogen Receptor-{beta} Is Critical to Granulosa Cell Differentiation and the Ovulatory Response to Gonadotropins Endocrinology, August 1, 2005; 146(8): 3247 - 3262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Garcia-Campayo, I. Boime, X. Ma, D. Daphna-Iken, and T. R. Kumar A Single-Chain Tetradomain Glycoprotein Hormone Analog Elicits Multiple Hormone Activities In Vivo Biol Reprod, February 1, 2005; 72(2): 301 - 308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J H Hampton, J F Bader, W R Lamberson, M F Smith, R S Youngquist, and H A Garverick Gonadotropin requirements for dominant follicle selection in GnRH agonist-treated cows Reproduction, June 1, 2004; 127(6): 695 - 703. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Alam, E. T. Maizels, Y. Park, S. Ghaey, Z. J. Feiger, N. S. Chandel, and M. Hunzicker-Dunn Follicle-stimulating Hormone Activation of Hypoxia-inducible Factor-1 by the Phosphatidylinositol 3-Kinase/AKT/Ras Homolog Enriched in Brain (Rheb)/Mammalian Target of Rapamycin (mTOR) Pathway Is Necessary for Induction of Select Protein Markers of Follicular Differentiation J. Biol. Chem., May 7, 2004; 279(19): 19431 - 19440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Adriaens, R. Cortvrindt, and J. Smitz Differential FSH exposure in preantral follicle culture has marked effects on folliculogenesis and oocyte developmental competence Hum. Reprod., February 1, 2004; 19(2): 398 - 408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Burns, J. E. Agno, L. Chen, B. Haupt, S. C. Ogbonna, K. S. Korach, and M. M. Matzuk Sexually Dimorphic Roles of Steroid Hormone Receptor Signaling in Gonadal Tumorigenesis Mol. Endocrinol., October 1, 2003; 17(10): 2039 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Burns, G. E. Owens, S. C. Ogbonna, J. H. Nilson, and M. M. Matzuk Expression Profiling Analyses of Gonadotropin Responses and Tumor Development in the Absence of Inhibins Endocrinology, October 1, 2003; 144(10): 4492 - 4507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Balla, N. Danilovich, Y. Yang, and M. R. Sairam Dynamics of Ovarian Development in the FORKO Immature Mouse: Structural and Functional Implications for Ovarian Reserve Biol Reprod, October 1, 2003; 69(4): 1281 - 1293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Meduri, P. Touraine, I. Beau, O. Lahuna, A. Desroches, M. C. Vacher-Lavenu, F. Kuttenn, and M. Misrahi Delayed Puberty and Primary Amenorrhea Associated with a Novel Mutation of the Human Follicle-Stimulating Hormone Receptor: Clinical, Histological, and Molecular Studies J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3491 - 3498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Grieshaber, C. Ko, S. S. Grieshaber, I. Ji, and T. H. Ji Follicle-Stimulating Hormone-Responsive Cytoskeletal Genes in Rat Granulosa Cells: Class I {beta}-Tubulin, Tropomyosin-4, and Kinesin Heavy Chain Endocrinology, January 1, 2003; 144(1): 29 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Harlow, L. Davidson, K. H. Burns, C. Yan, M. M. Matzuk, and S. G. Hillier FSH and TGF-{beta} Superfamily Members Regulate Granulosa Cell Connective Tissue Growth Factor Gene Expression in Vitro and in Vivo Endocrinology, September 1, 2002; 143(9): 3316 - 3325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Burns, G. E. Owens, J. M. Fernandez, J. H. Nilson, and M. M. Matzuk Characterization of Integrin Expression in the Mouse Ovary Biol Reprod, September 1, 2002; 67(3): 743 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Burns and M. M. Matzuk Minireview: Genetic Models for the Study of Gonadotropin Actions Endocrinology, August 1, 2002; 143(8): 2823 - 2835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Richards, S. C. Sharma, A. E. Falender, and Y. H. Lo Expression of FKHR, FKHRL1, and AFX Genes in the Rodent Ovary: Evidence for Regulation by IGF-I, Estrogen, and the Gonadotropins Mol. Endocrinol., March 1, 2002; 16(3): 580 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Richards, D. L. Russell, S. Ochsner, M. Hsieh, K. H. Doyle, A. E. Falender, Y. K. Lo, and S. C. Sharma Novel Signaling Pathways That Control Ovarian Follicular Development, Ovulation, and Luteinization Recent Prog. Horm. Res., January 1, 2002; 57(1): 195 - 220. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
