help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tiffoche, C.
Right arrow Articles by Thieulant, M.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tiffoche, C.
Right arrow Articles by Thieulant, M.-L.
Endocrinology Vol. 142, No. 9 4106-4119
Copyright © 2001 by The Endocrine Society

ARTICLES

Novel Intronic Promoter in the Rat ER{alpha} Gene Responsible for the Transient Transcription of a Variant Receptor

Christophe Tiffoche, Colette Vaillant, Diane Schausi and Marie-Lise Thieulant

Université de Rennes I, Interactions Cellulaires et Moléculaires, Equipe Information et Programmation Cellulaires, Centre National de la Recherche Scientifique UMR 6026, Campus de Beaulieu, Rennes Cedex 35042, France

Address all correspondence and requests for reprints to: Dr. M.-L. Thieulant, Equipe Information et Programmation Cellulaires, UMR 6026, Bat 13, Campus de Beaulieu, 35042 Rennes cedex, France. E-mail: Marie-Lise.Thieulant{at}univ-rennes1.fr

To analyze the molecular origin of an ER variant, the truncated ER product-1, transiently expressed at the proestrus in lactotrope cells, we generated a 2.5-kb sequence of a genomic region upstream and downstream the specific sequence truncated ER product-1. Genomic Southern blot analysis showed that truncated ER product-1 is spliced from a noncoding leader exon localized within the intron 4 of the ER {alpha} gene. Analysis of the promoter sequence revealed the presence of a major transcriptional start site, a canonical TATA box and putative cis regulatory elements for pituitary specific expression as well as an E-responsive element. In transient transfection, the truncated ER product-1 promoter was transcriptionally the most active in the lactotrope cell lines (MMQ). Analysis of truncated ER product-1 functionality showed that: 1) the protein inhibited ER{alpha} binding to the E-responsive element in electromobility shift assays, 2) inhibited the E2 binding to ER{alpha} in binding assays, 3) the truncated ER product-1/ER{alpha} complex antagonized the transcriptional activity elicited by E2, 4) nuclear localization of green fluorescent protein-ER{alpha} was altered in Chinese hamster ovary cell lines stably expressing truncated ER product-1. Collectively, these data demonstrated that the protein exerts full dominant negative activity against ER{alpha}. Moreover, truncated ER product-1/ER {alpha} complex also repressed the activity of all promoters tested to date, suggesting a general inhibitory effect toward transcription. In conclusion, the data suggest that truncated ER product-1 could regulate estrogen signaling via a specific promoter in lactotrope cells.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
W. M. Bryant, M. A. Gibson, and M. A. Shupnik
Stimulation of the Novel Estrogen Receptor-{alpha} Intronic TERP-1 Promoter by Estrogens, Androgen, Pituitary Adenylate Cyclase-Activating Peptide, and Forskolin, and Autoregulation by TERP-1 Protein
Endocrinology, January 1, 2006; 147(1): 543 - 551.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H.-b. Liu, K. K. Loo, K. Palaszynski, J. Ashouri, D. B. Lubahn, and R. R. Voskuhl
Estrogen Receptor {alpha} Mediates Estrogen's Immune Protection in Autoimmune Disease
J. Immunol., December 15, 2003; 171(12): 6936 - 6940.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Y. Lin, E. M. Resnick, and M. A. Shupnik
Truncated Estrogen Receptor Product-1 Stimulates Estrogen Receptor {alpha} Transcriptional Activity by Titration of Repressor Proteins
J. Biol. Chem., October 3, 2003; 278(40): 38125 - 38131.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. Schausi, C. Tiffoche, and M.-L. Thieulant
Regulation of the Intronic Promoter of Rat Estrogen Receptor {alpha} Gene, Responsible for Truncated Estrogen Receptor Product-1 Expression
Endocrinology, July 1, 2003; 144(7): 2845 - 2855.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. A. Schreihofer, D. F. Rowe, E. F. Rissman, E. M. Scordalakes, J.-a. Gustafsson, and M. A. Shupnik
Estrogen Receptor-{alpha} (ER{alpha}), But Not ER{beta}, Modulates Estrogen Stimulation of the ER{alpha}-Truncated Variant, TERP-1
Endocrinology, November 1, 2002; 143(11): 4196 - 4202.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Vaillant, F. Chesnel, D. Schausi, C. Tiffoche, and M.-L. Thieulant
Expression of Estrogen Receptor Subtypes in Rat Pituitary Gland during Pregnancy and Lactation
Endocrinology, November 1, 2002; 143(11): 4249 - 4258.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2001 by The Endocrine Society