This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Paciga, M. Articles by Wagner, G. F. Search for Related Content PubMed PubMed Citation Articles by Paciga, M. Articles by Wagner, G. F.

Ovarian Stanniocalcin Is Structurally Unique in Mammals and Its Production and Release Are Regulated through the Luteinizing Hormone Receptor

Departments of Physiology (M.P., A.J.W., G.F.W.), Oncology (G.E.D.), and Obstetrics and Gynecology (A.J.W., G.E.D.), Faculty of Medicine and Dentistry, University of Western Ontario, and London Regional Cancer Center (G.E.D.), London, Ontario, Canada N6A 5C1

Address all correspondence and requests for reprints to: Dr. Graham F. Wagner, Department of Physiology, Faculty of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada N6A 5C1. E-mail: graham.wagner{at}fmd.uwo.ca.

Stanniocalcin (STC) is a recently discovered mammalian hormone that is widely distributed in many tissues. In rodents the STC gene is most highly expressed in ovary, specifically in androgen-producing thecal and interstitial cells. In addition, ovarian levels of expression rise 15-fold over pregnancy. The objective of this study was to develop a primary culture system for ovarian thecal-interstitial cells (TICs) to identify factors governing STC production and release. We used highly purified primary cultures of rat and bovine TICs, the purity of which was routinely assessed with antigenic and enzymatic markers. The functionality of cells was assured by their responsiveness to LH in the form of progesterone release. We found that forskolin significantly increased STC gene expression and secretion by both rat and bovine TICs, an effect that was only replicated by human (h) chorionic gonadotropin (CG). Coincubation of TICs with hCG and phosphodiesterase inhibitors further increased STC secretion, whereas coincubation of TICs with hCG and protein kinase A inhibitors attenuated hCG-stimulated release. Intriguingly, ovarian STC proved to be substantially larger than the 50-kDa homodimer produced in most other tissues. These results indicate that ovarian STC is physically distinct, a feature that could explain its presence in serum during pregnancy and lactation.

This article has been cited by other articles:

				D. Zaidi, K. A. James, and G. F. Wagner Passive immunization of lactating mice with stanniocalcin-1 antiserum reduces mammary gland development, milk fat content, and postnatal pup growth Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E974 - E981. [Abstract] [Full Text] [PDF]

				L.-J. Xiao, J.-X. Yuan, X.-X. Song, Y.-C. Li, Z.-Y. Hu, and Y.-X. Liu Expression and regulation of stanniocalcin 1 and 2 in rat uterus during embryo implantation and decidualization. Reproduction, June 1, 2006; 131(6): 1137 - 1149. [Abstract] [Full Text] [PDF]

				C. P. Hasilo, C. R. McCudden, J. R. J. Gillespie, K. A. James, E. R. Hirvi, D. Zaidi, and G. F. Wagner Nuclear targeting of stanniocalcin to mammary gland alveolar cells during pregnancy and lactation Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E634 - E642. [Abstract] [Full Text] [PDF]

				M. Paciga, E. R. Hirvi, K. James, and G. F. Wagner Characterization of big stanniocalcin variants in mammalian adipocytes and adrenocortical cells Am J Physiol Endocrinol Metab, August 1, 2005; 289(2): E197 - E205. [Abstract] [Full Text] [PDF]

				M. Paciga, G. E. DiMattia, and G. F. Wagner Regulation of Luteal Cell Big Stanniocalcin Production and Secretion Endocrinology, September 1, 2004; 145(9): 4204 - 4212. [Abstract] [Full Text] [PDF]

				C.-W. Luo, K. Kawamura, C. Klein, and A. J. W. Hsueh Paracrine Regulation of Ovarian Granulosa Cell Differentiation by Stanniocalcin (STC) 1: Mediation through Specific STC1 Receptors Mol. Endocrinol., August 1, 2004; 18(8): 2085 - 2096. [Abstract] [Full Text] [PDF]

				M. Paciga, C. R. McCudden, C. Londos, G. E. DiMattia, and G. F. Wagner Targeting of Big Stanniocalcin and Its Receptor to Lipid Storage Droplets of Ovarian Steroidogenic Cells J. Biol. Chem., December 5, 2003; 278(49): 49549 - 49554. [Abstract] [Full Text] [PDF]

				C. Zlot, G. Ingle, J. Hongo, S. Yang, Z. Sheng, R. Schwall, N. Paoni, F. Wang, F. V. Peale Jr., and M. E. Gerritsen Stanniocalcin 1 Is an Autocrine Modulator of Endothelial Angiogenic Responses to Hepatocyte Growth Factor J. Biol. Chem., November 28, 2003; 278(48): 47654 - 47659. [Abstract] [Full Text] [PDF]

				H. Y. Yeung, D. K. O. Chan, N. K. Mak, G. F. Wagner, and C. K. C. Wong Identification of Signal Transduction Pathways that Modulate Dibutyryl Cyclic Adenosine Monophosphate Activation of Stanniocalcin Gene Expression in Neuroblastoma Cells Endocrinology, October 1, 2003; 144(10): 4446 - 4452. [Abstract] [Full Text] [PDF]