| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
ARTICLE |
Department of Periodontics/Prevention/Geriatrics (B.D., H.-L.C., A.J.K., L.K.M.),, School of Dentistry, and Unit for Laboratory Animal Medicine (E.T.K.) and Department of Pathology (E.T.K., L.K.M.), Medical School, University of Michigan Ann Arbor, Michigan 48109; and Department of Periodontology, Faculty of Dentistry, Hacettepe University (B.D.), Ankara, Turkey 06100
Address all correspondence and requests for reprints to: Dr. Laurie K. McCauley, Department of Periodontics/Prevention/Geriatrics, University of Michigan, 1011 North University Avenue, Ann Arbor, Michigan 48109-1078. E-mail: mccauley{at}umich.edu.
PTH has anabolic and catabolic actions in bone that are not clearly understood. The protooncogene c-fos and other activating protein 1 family members are critical transcriptional mediators in bone, and c-fos is up-regulated by PTH. The purpose of this study was to examine the mechanisms of PTH and the role of c-fos in PTH-mediated anabolic actions in bone. Mice with ablation of c-fos (-/-) and their wild-type (+/+) and heterozygous (+/-) littermates were administered PTH for 17 d. The +/+ mice had increased femoral bone mineral density (BMD), whereas -/- mice had reduced BMD after PTH treatment. PTH increased the ash weight of +/+ and +/-, but not -/-, femurs and decreased the calcium content of -/-, but not +/+ or +/-, femurs. Histomorphometric analysis showed that PTH increased trabecular bone volume in c-fos +/+, +/- vertebrae, but, in contrast, decreased trabecular bone in -/- vertebrae. Serum calcium levels in +/+ mice were greater than those in -/- mice, and PTH increased calcium in -/- mice. Histologically, PTH resulted in an exacerbation of the already widened growth plate and zone of hypertrophic chondrocytes but not the proliferating zone in -/- mice. PTH also increased calvarial thickness in +/+ mice, but not -/- mice. The c-fos -/- mice had lower bone sialoprotein and osteocalcin (OCN), but unaltered PTH-1 receptor mRNA expression in calvaria, suggesting an alteration in extracellular matrix. Acute PTH injection (8 h) resulted in a decrease in osteocalcin mRNA expression in wild-type, but unaltered expression in -/-, calvaria. These data indicate that c-fos plays a critical role in the anabolic actions of PTH during endochondral bone growth.
This article has been cited by other articles:
 |
|
 |
|
  T. Kawano, N. Troiano, D. J. Adams, J. Jun Wu, B.-h. Sun, and K. Insogna The Anabolic Response to Parathyroid Hormone Is Augmented in Rac2 Knockout Mice Endocrinology, August 1, 2008; 149(8): 4009 - 4015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shimada, P. A. Greer, A. P. McMahon, M. L. Bouxsein, and E. Schipani In Vivo Targeted Deletion of Calpain Small Subunit, Capn4, in Cells of the Osteoblast Lineage Impairs Cell Proliferation, Differentiation, and Bone Formation J. Biol. Chem., July 25, 2008; 283(30): 21002 - 21010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yu, R. T. Franceschi, M. Luo, X. Zhang, D. Jiang, Y. Lai, Y. Jiang, J. Zhang, and G. Xiao Parathyroid Hormone Increases Activating Transcription Factor 4 Expression and Activity in Osteoblasts: Requirement for Osteocalcin Gene Expression Endocrinology, April 1, 2008; 149(4): 1960 - 1968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Raggatt, L. Qin, J. Tamasi, S. C. Jefcoat Jr., E. Shimizu, N. Selvamurugan, F. Y. Liew, L. Bevelock, J. H. M. Feyen, and N. C. Partridge Interleukin-18 Is Regulated by Parathyroid Hormone and Is Required for Its Bone Anabolic Actions J. Biol. Chem., March 14, 2008; 283(11): 6790 - 6798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-H. Wang, Y. Liu, and D. W. Rowe Effects of transient PTH on early proliferation, apoptosis, and subsequent differentiation of osteoblast in primary osteoblast cultures Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E594 - E603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Karsdal and K. Henriksen Osteoclasts Control Osteoblast Activity IBMS BoneKEy, January 1, 2007; 4(1): 19 - 24. [Full Text] [PDF]
|
|
|
|
|
|
A. J. Koh, B. Demiralp, K. G. Neiva, J. Hooten, R. M. Nohutcu, H. Shim, N. S. Datta, R. S. Taichman, and L. K. McCauley Cells of the Osteoclast Lineage as Mediators of the Anabolic Actions of Parathyroid Hormone in Bone Endocrinology, November 1, 2005; 146(11): 4584 - 4596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zayzafoon, K. Fulzele, and J. M. McDonald Calmodulin and Calmodulin-dependent Kinase II{alpha} Regulate Osteoblast Differentiation by Controlling c-fos Expression J. Biol. Chem., February 25, 2005; 280(8): 7049 - 7059. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Chattopadhyay, S. Yano, J. Tfelt-Hansen, P. Rooney, D. Kanuparthi, S. Bandyopadhyay, X. Ren, E. Terwilliger, and E. M. Brown Mitogenic Action of Calcium-Sensing Receptor on Rat Calvarial Osteoblasts Endocrinology, July 1, 2004; 145(7): 3451 - 3462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Jiang, R. T. Franceschi, H. Boules, and G. Xiao Parathyroid Hormone Induction of the Osteocalcin Gene: REQUIREMENT FOR AN OSTEOBLAST-SPECIFIC ELEMENT 1 SEQUENCE IN THE PROMOTER AND INVOLVEMENT OF MULTIPLE SIGNALING PATHWAYS J. Biol. Chem., February 13, 2004; 279(7): 5329 - 5337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kumar, H. Kishimoto, H. L. Chua, S. Badve, K. D. Miller, R. M. Bigsby, and H. Nakshatri Interleukin-1{alpha} Promotes Tumor Growth and Cachexia in MCF-7 Xenograft Model of Breast Cancer Am. J. Pathol., December 1, 2003; 163(6): 2531 - 2541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zeng, L. K. McCauley, and C.-Y. Wang Hepatocyte Growth Factor Inhibits Anoikis by Induction of Activator Protein 1-dependent Cyclooxygenase-2. IMPLICATION IN HEAD AND NECK SQUAMOUS CELL CARCINOMA PROGRESSION J. Biol. Chem., December 13, 2002; 277(51): 50137 - 50142. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
