This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kawamura, K. Articles by Tanaka, T. Search for Related Content PubMed PubMed Citation Articles by Kawamura, K. Articles by Tanaka, T.

Leptin Promotes the Development of Mouse Preimplantation Embryos in Vitro

Department of Obstetrics and Gynecology (K.K., N.S., J.F., J.K., H.T., T.T.), Department of Medical Information Science (A.N.), Akita University School of Medicine, and Akita University College of Allied Medical Science (H.K.), Akita 010-0041, Japan

Address all correspondence and requests for reprints to: Kazuhiro Kawamura, Department of Obstetrics and Gynecology, Akita University School of Medicine, Hondo 1-1-1, Akita 010-8543, Japan. E-mail: . kawamurak{at}obgyn.med.akita-u.ac.jp

Leptin acts as a modulator of diverse reproductive functions, and recent studies have implicated involvement of leptin in the early embryo development in mammal. The aim of this study was to investigate the expression of leptin and its receptor (OB-R) in mouse oocyte and preimplantation embryo, and to examine whether leptin influenced the early embryo development. Leptin mRNA was detected in blastocyst and hatched blastocyst, and two splice variants of OB-R (OB-Ra and OB-Rb) mRNAs were detected in oocytes, 1-cell, 2-cell, morula, blastocyst, and hatched blastocyst. As for the origin of leptin, both leptin mRNA and protein were identified in the oviduct epithelium and endometrium of pregnant mouse. In the pregnant mouse, the levels of leptin in uterine fluid were higher than those in nonpregnant mouse. Addition of leptin to embryo culture media promotes the development from 2-cell stage embryos to the blastocysts, fully expanded blastocysts and hatched blastocysts. This effect was neutralized by an antibody against the extracellular domain of OB-R. Leptin significantly increased the total cell number of blastocysts, and the effect was preferentially observed in the trophectoderm. These findings raise the possibility of a paracrine/autocrine leptin signaling system regulating the development of mouse preimplantation embryo.

This article has been cited by other articles:

				A. K. Nath, R. M. Brown, M. Michaud, M. R. Sierra-Honigmann, M. Snyder, and J. A. Madri Leptin affects endocardial cushion formation by modulating EMT and migration via Akt signaling cascades J. Cell Biol., April 21, 2008; 181(2): 367 - 380. [Abstract] [Full Text] [PDF]

				M. Archanco, J. Gomez-Ambrosi, M. Tena-Sempere, G. Fruhbeck, and M. A. Burrell Expression of Leptin and Adiponectin in the Rat Oviduct J. Histochem. Cytochem., October 1, 2007; 55(10): 1027 - 1037. [Abstract] [Full Text] [PDF]

				F. F. Paula-Lopes, M. Boelhauve, F. A. Habermann, F. Sinowatz, and E. Wolf Leptin Promotes Meiotic Progression and Developmental Capacity of Bovine Oocytes Via Cumulus Cell-Independent and -Dependent Mechanisms Biol Reprod, March 1, 2007; 76(3): 532 - 541. [Abstract] [Full Text] [PDF]

				M. Herrid, V. L. Nguyen, G. Hinch, and J. R McFarlane Leptin has concentration and stage-dependent effects on embryonic development in vitro. Reproduction, August 1, 2006; 132(2): 247 - 256. [Abstract] [Full Text] [PDF]

				K.-F. Lee, J.-S. Xu, Y.-L. Lee, and W. S. B. Yeung Demilune Cell and Parotid Protein from Murine Oviductal Epithelium Stimulates Preimplantation Embryo Development Endocrinology, January 1, 2006; 147(1): 79 - 87. [Abstract] [Full Text] [PDF]

				K. Kawamura, J. Fukuda, Y. Shimizu, H. Kodama, and T. Tanaka Survivin Contributes to the Anti-Apoptotic Activities of Transforming Growth Factor alpha in Mouse Blastocysts Through Phosphatidylinositol 3'-Kinase Pathway Biol Reprod, December 1, 2005; 73(6): 1094 - 1101. [Abstract] [Full Text] [PDF]

				T. Aparicio, S. Kermorgant, D. Darmoul, S. Guilmeau, K. Hormi, D. Mahieu-Caputo, and T. Lehy Leptin and Ob-Rb Receptor Isoform in the Human Digestive Tract during Fetal Development J. Clin. Endocrinol. Metab., November 1, 2005; 90(11): 6177 - 6184. [Abstract] [Full Text] [PDF]

				M. Boelhauve, F. Sinowatz, E. Wolf, and F. F. Paula-Lopes Maturation of Bovine Oocytes in the Presence of Leptin Improves Development and Reduces Apoptosis of In Vitro-Produced Blastocysts Biol Reprod, October 1, 2005; 73(4): 737 - 744. [Abstract] [Full Text] [PDF]

				K. Kawamura, J. Fukuda, J. Kumagai, Y. Shimizu, H. Kodama, A. Nakamura, and T. Tanaka Gonadotropin-Releasing Hormone I Analog Acts as an Antiapoptotic Factor in Mouse Blastocysts Endocrinology, September 1, 2005; 146(9): 4105 - 4116. [Abstract] [Full Text] [PDF]

				Y. Honda, H. Tanikawa, J. Fukuda, K. Kawamura, N. Sato, T. Sato, Y. Shimizu, H. Kodama, and T. Tanaka Expression of Smac/DIABLO in mouse preimplantation embryos and its correlation to apoptosis and fragmentation Mol. Hum. Reprod., March 1, 2005; 11(3): 183 - 188. [Abstract] [Full Text] [PDF]

				M. P. Ramos, B. R. Rueda, P. C. Leavis, and R. R. Gonzalez Leptin Serves as an Upstream Activator of an Obligatory Signaling Cascade in the Embryo-Implantation Process Endocrinology, February 1, 2005; 146(2): 694 - 701. [Abstract] [Full Text] [PDF]

				M. Maccarrone, E. Fride, T. Bisogno, M. Bari, M.G. Cascio, N. Battista, A. Finazzi Agro, R. Suris, R. Mechoulam, and V. Di Marzo Up-regulation of the endocannabinoid system in the uterus of leptin knockout (ob/ob) mice and implications for fertility Mol. Hum. Reprod., January 1, 2005; 11(1): 21 - 28. [Abstract] [Full Text] [PDF]

				J. Craig, H. Zhu, P. W. Dyce, J. Petrik, and J. Li Leptin Enhances Oocyte Nuclear and Cytoplasmic Maturation via the Mitogen-Activated Protein Kinase Pathway Endocrinology, November 1, 2004; 145(11): 5355 - 5363. [Abstract] [Full Text] [PDF]

				P. Fedorcsak, P. O. Dale, R. Storeng, G. Ertzeid, S. Bjercke, N. Oldereid, A. K. Omland, T. Abyholm, and T. Tanbo Impact of overweight and underweight on assisted reproduction treatment Hum. Reprod., November 1, 2004; 19(11): 2523 - 2528. [Abstract] [Full Text] [PDF]

				J. E. Swain, R. L. Dunn, D. McConnell, J. Gonzalez-Martinez, and G. D. Smith Direct Effects of Leptin on Mouse Reproductive Function: Regulation of Follicular, Oocyte, and Embryo Development Biol Reprod, November 1, 2004; 71(5): 1446 - 1452. [Abstract] [Full Text] [PDF]

				R. R. Gonzalez, B. R. Rueda, M. P. Ramos, R. D. Littell, S. Glasser, and P. C. Leavis Leptin-Induced Increase in Leukemia Inhibitory Factor and Its Receptor by Human Endometrium Is Partially Mediated by Interleukin 1 Receptor Signaling Endocrinology, August 1, 2004; 145(8): 3850 - 3857. [Abstract] [Full Text] [PDF]

				A. Cervero, J. A. Horcajadas, J. MartIn, A. Pellicer, and C. Simon The Leptin System during Human Endometrial Receptivity and Preimplantation Development J. Clin. Endocrinol. Metab., May 1, 2004; 89(5): 2442 - 2451. [Abstract] [Full Text] [PDF]

				N. K. Ryan, K. H. Van der Hoek, S. A. Robertson, and R. J. Norman Leptin and Leptin Receptor Expression in the Rat Ovary Endocrinology, November 1, 2003; 144(11): 5006 - 5013. [Abstract] [Full Text] [PDF]

				P. Fedorcsak and R. Storeng Effects of Leptin and Leukemia Inhibitory Factor on Preimplantation Development and STAT3 Signaling of Mouse Embryos In Vitro Biol Reprod, November 1, 2003; 69(5): 1531 - 1538. [Abstract] [Full Text] [PDF]

				K. Kawamura, N. Sato, J. Fukuda, H. Kodama, J. Kumagai, H. Tanikawa, A. Nakamura, Y. Honda, T. Sato, and T. Tanaka Ghrelin Inhibits the Development of Mouse Preimplantation Embryos in Vitro Endocrinology, June 1, 2003; 144(6): 2623 - 2633. [Abstract] [Full Text] [PDF]

				R. R. Gonzalez, K. Leary, J. C. Petrozza, and P. C. Leavis Leptin regulation of the interleukin-1 system in human endometrial cells Mol. Hum. Reprod., March 1, 2003; 9(3): 151 - 158. [Abstract] [Full Text] [PDF]