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Cyclosporine A and FK506 Inhibit Transcriptional Activity of the Human Mineralocorticoid Receptor: A Cell-Based Model to Investigate Partial Aldosterone Resistance in Kidney Transplantation

INSERM, U-478, IFR02, Faculté de Médecine Xavier Bichat (C.E.D., S.V., N.F., M.L.), 75870 Paris, France; and Klinik für Nephrologie und Rheumatologie, Heinrich Heine Universität (C.E.D., P.J.H., B.G.), 40225 Dusseldorf, Germany

Address all correspondence and requests for reprints to: Dr. Marc Lombès, INSERM, U-478, IFR02, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, BP 416, 75870 Paris Cedex 18, France. E-mail: . mlombes{at}bichat.inserm.fr

Abstract

Renal transplant recipients treated with cyclosporine A (CsA) and FK506 (tacrolimus) develop signs of hypoaldosteronism despite normal plasma aldosterone levels, suggesting a relative resistance of the distal nephron to aldosterone action. To examine the effects of immunosuppressants on human MR (hMR) function, we established the M cell model, renal tubular cells stably transfected with hMR. Upon CsA and FK506 administration, hMR mRNA levels and aldosterone binding in M cells remained unchanged (maximum number of sites, 80 fmol/mg protein; K_d = 1 nM). Aldosterone-dependent intracellular localization of green fluorescent protein-hMR was not affected by immunosuppressants. A major impact of CsA or FK506 on the multidrug resistance gene product in cellular accumulation of aldosterone was also excluded. In contrast, aldosterone-stimulated hMR transcriptional activity was reduced to 53 ± 11.2% (P < 0.03) after pretreatment of M cells for 3 d with CsA and to 71 ± 9.6% (P < 0.05) after pretreatment with FK506. These effects were both time and concentration dependent (IC₅₀ of CsA, 10^-6 M; IC₅₀ of FK506, 10^-5 M) and needed at least 2 d to develop. Such an inhibitory effect does not depend on the N-terminal part of hMR, as CsA also reduced transcriptional activity of a 1–453 deletion mutant of hMR. Our results demonstrate that immunosuppressants inhibit hMR transcriptional activity without affecting hMR expression, aldosterone binding properties, and hMR nucleocytoplasmic trafficking. They suggest that ion transport alterations in renal graft recipients are in part induced by impaired hMR function.

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