This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Patisaul, H. B. Articles by Young, L. J. Search for Related Content PubMed PubMed Citation Articles by Patisaul, H. B. Articles by Young, L. J.

Genistein Affects ERß- But Not ER-Dependent Gene Expression in the Hypothalamus

Center for Behavioral Neuroscience (H.B.P., P.L.W., L.J.Y.), Department of Anthropology (M.M., P.L.W.), and Department of Psychiatry and Behavioral Sciences (L.J.Y.), Emory University, Atlanta, Georgia 30329

Address all correspondence and requests for reprints to: Heather B. Patisaul, Ph.D., Center for Behavioral Neuroscience, Emory University, 954 Gatewood Road Northeast, Atlanta, Georgia 30329. E-mail: hbeaupr{at}emory.edu.

Isoflavone phytoestrogens are growing increasingly popular because of their reported cardiovascular and anticarcinogenic properties, but the effects of these compounds in the brain are largely unknown. In a previous study, we found that an isoflavone supplement, containing a mixture of soy phytoestrogens, inhibited estrogen-dependent female sexual behavior and was antiestrogenic for both ER- and ERß-dependent gene expression in the hypothalamus. Here we examined the impact of the soy isoflavone genistein, a major component of the supplement, on estrogen-dependent female sexual behavior and ER- and ERß-dependent gene expression in the rat brain. Genistein, at a dietary concentration of 100 or 500 ppm had no effect on lordosis behavior in rats. However, at 500 ppm genistein had differential activity through ER and ERß in the hypothalamus. Genistein had no effect, in either the presence or absence of 17ß-E2, on oxytocin receptor density in the ventromedial nucleus of the hypothalamus, an estrogen-dependent action thought to be regulated via ER. However, genistein increased ERß mRNA expression in the paraventricular nucleus of the hypothalamus by 24%, whereas 17ß-E2 decreased ERß mRNA expression by 26%, a process likely mediated by ERß itself. These results suggest that at this dose, genistein has antiestrogenic action through ERß in the paraventriculr nucleus but negligible activity through ER in the brain.

This article has been cited by other articles:

				H. B. Patisaul, A. E. Fortino, and E. K. Polston Sex Differences in Serotonergic But Not {gamma}-Aminobutyric Acidergic (GABA) Projections to the Rat Ventromedial Nucleus of the Hypothalamus Endocrinology, January 1, 2008; 149(1): 397 - 408. [Abstract] [Full Text] [PDF]

				W. M. Bryant, M. A. Gibson, and M. A. Shupnik Stimulation of the Novel Estrogen Receptor-{alpha} Intronic TERP-1 Promoter by Estrogens, Androgen, Pituitary Adenylate Cyclase-Activating Peptide, and Forskolin, and Autoregulation by TERP-1 Protein Endocrinology, January 1, 2006; 147(1): 543 - 551. [Abstract] [Full Text] [PDF]

				R. G. Mishra, F. Z. Stanczyk, K. A. Burry, S. Oparil, B. S. Katzenellenbogen, M. L. Nealen, J. A. Katzenellenbogen, and R. K. Hermsmeyer Metabolite ligands of estrogen receptor-{beta} reduce primate coronary hyperreactivity Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H295 - H303. [Abstract] [Full Text] [PDF]

				S. Gingerich and T. L. Krukoff Estrogen Modulates Endothelial and Neuronal Nitric Oxide Synthase Expression via an Estrogen Receptor {beta}-Dependent Mechanism in Hypothalamic Slice Cultures Endocrinology, July 1, 2005; 146(7): 2933 - 2941. [Abstract] [Full Text] [PDF]

				M. Yan, M. E. E. Jones, M. Hernandez, D. Liu, E. R. Simpson, and C. Chen Functional Modification of Pituitary Somatotropes in the Aromatase Knockout Mouse and the Effect of Estrogen Replacement Endocrinology, February 1, 2004; 145(2): 604 - 612. [Abstract] [Full Text] [PDF]

				Y. Ikeda, A. Nagai, M.-A. Ikeda, and S. Hayashi Sexually Dimorphic and Estrogen-Dependent Expression of Estrogen Receptor {beta} in the Ventromedial Hypothalamus during Rat Postnatal Development Endocrinology, November 1, 2003; 144(11): 5098 - 5104. [Abstract] [Full Text] [PDF]

				S. L. Hardy, G. M. Anderson, M. Valent, J. M. Connors, and R. L. Goodman Evidence That Estrogen Receptor Alpha, but Not Beta, Mediates Seasonal Changes in the Response of the Ovine Retrochiasmatic Area to Estradiol Biol Reprod, March 1, 2003; 68(3): 846 - 852. [Abstract] [Full Text] [PDF]