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Immunohistochemical Localization of Microsomal PGE Synthase-1 and Cyclooxygenases in Male Mouse Reproductive Organs

Department of Molecular Behavioral Biology (M.L., C.J.M., N.E., S.M., B.K.K., Y.U.) and Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (Y.U.), Osaka Bioscience Institute, Osaka 565-0874, Japan; and Department of Dairy and Animal Science (G.J.K.), J. O. Almquist Research Center, Pennsylvania State University, University Park, Pennsylvania 16802

Address all correspondence and requests for reprints to: Dr. Yoshihiro Urade, Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Furuedai 6-2-4, Suita, Osaka 565-0874, Japan. E-mail: . uradey{at}obi.or.jp

We investigated the tissue distribution and cellular localization of microsomal PGE synthase-1 (mPGES-1) and cyclooxygenase (COX)-1 and -2 in the male mouse reproductive organs. Northern blotting revealed that the mPGES-1 mRNA was expressed intensely in the epididymis and weakly in the lung, spleen, skin, kidney, colon, and brain. In the male reproductive tract, the expression of mPGES-1 increased from the testis to the cauda epididymis and was highest in the vas deferens when examined by Northern blotting, RT-PCR, and Western blotting. By immunohistochemistry, mPGES-1 was detected in Leydig cells of the testis and in epithelial cells of the epididymis, vas deferens, and seminal vesicles. In addition, the caput and cauda regions of the epididymis and the vas deferens in this order showed a progressive increase in the expression of COX-1 mRNA and immunoreactivity, whereas COX-2 was dominantly expressed in the vas deferens. COX-1 was localized in epithelial cells of the caput, corpus and cauda epididymis and of the vas deferens, and COX-2 was evident in epithelial cells of the distal cauda epididymis and vas deferens. These results show that mPGES-1 is expressed coordinately with COX-1 and COX-2 and is involved in PGE₂ production in male genital organs.

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