This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Alexandre, D. Articles by Anouar, Y. Search for Related Content PubMed PubMed Citation Articles by Alexandre, D. Articles by Anouar, Y.

Novel Splice Variants of Type I Pituitary Adenylate Cyclase-Activating Polypeptide Receptor in Frog Exhibit Altered Adenylate Cyclase Stimulation and Differential Relative Abundance

European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, Institut National de la Santé et de la Recherche Médicale, U-413, Unité Affiliée Centre National de la Recherche Scientifique, University of Rouen (D.A., H.V., L.G., V.T., S.J., Y.A.), 76821 Mont Saint Aignan, France; Institut National de la Recherche Scientifique/Institut Armand Frappier, University of Quebec (A.F.), H9R 1GG Montréal, Canada

Address all correspondence and requests for reprints to: Dr. Youssef Anouar, European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, Institut National de la Santé et de la Recherche Médicale, U-413, Unité Affiliée Centre National de la Recherche, University of Rouen, 76821 Mont Saint Aignan, France. E-mail: . youssef.anouar{at}univ-rouen.fr

Pituitary adenylate cyclase-activating polypeptide (PACAP) exerts its various effects through activation of two types of G protein-coupled receptors, a receptor with high affinity for PACAP named PAC1-R and two receptors exhibiting similar affinity for both PACAP and vasoactive intestinal polypeptide named VPAC1-R and VPAC2-R. Here, we report the characterization of PAC1-R and novel splice variants in the frog Rana ridibunda. The frog PAC1-R has 78% homology with human PAC1-R and is highly expressed in the central nervous system. Two splice variants of the frog receptor that display additional amino acid cassettes in the third intracellular loop were characterized. PAC1-R25 carries a 25-amino acid insertion that matches the hop cassette of the mammalian receptor, whereas PAC1-R41 carries a cassette with no homology to any mammalian PAC1-R variant. A third splice variant of PAC1-R, exhibiting a completely different intracellular C-terminal domain, named PAC1-Rmc has also been identified. Determination of cAMP formation in cells transfected with the cloned receptors showed that PACAP activated PAC1-R, PAC1-R25, and PAC1-R41 with similar potency. In contrast, PACAP failed to stimulate adenylate cyclase in cells transfected with PAC1-Rmc. Fusion of PAC1-R or PAC1-Rmc with the green fluorescent protein revealed that both receptors are expressed and targeted to the plasma membrane in transfected cells. The different PAC1-R variants are highly expressed in the frog brain and spinal cord and to a lesser extent in peripheral tissues, where only certain isoforms could be detected. The present data indicate that in frog, PACAP may act through different PAC1-R splice variants that differ in their G_s protein coupling and their abundance in various tissues.

This article has been cited by other articles:

				D. Vaudry, A. Falluel-Morel, S. Bourgault, M. Basille, D. Burel, O. Wurtz, A. Fournier, B. K. C. Chow, H. Hashimoto, L. Galas, et al. Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: 20 Years after the Discovery Pharmacol. Rev., September 1, 2009; 61(3): 283 - 357. [Abstract] [Full Text] [PDF]

				M. Ushiyama, R. Ikeda, H. Sugawara, M. Yoshida, K. Mori, K. Kangawa, K. Inoue, K. Yamada, and A. Miyata Differential Intracellular Signaling through PAC1 Isoforms as a Result of Alternative Splicing in the First Extracellular Domain and the Third Intracellular Loop Mol. Pharmacol., July 1, 2007; 72(1): 103 - 111. [Abstract] [Full Text] [PDF]

				J. Vendelin, V. Pulkkinen, M. Rehn, A. Pirskanen, A. Raisanen-Sokolowski, A. Laitinen, L. A. Laitinen, J. Kere, and T. Laitinen Characterization of GPRA, a Novel G Protein-Coupled Receptor Related to Asthma Am. J. Respir. Cell Mol. Biol., September 1, 2005; 33(3): 262 - 270. [Abstract] [Full Text] [PDF]

				C. Martinez, Y. Juarranz, C. Abad, A. Arranz, B. G. Miguel, F. Rosignoli, J. Leceta, and R. P. Gomariz Analysis of the role of the PAC1 receptor in neutrophil recruitment, acute-phase response, and nitric oxide production in septic shock J. Leukoc. Biol., May 1, 2005; 77(5): 729 - 738. [Abstract] [Full Text] [PDF]

				L. Grumolato, A. G. Elkahloun, H. Ghzili, D. Alexandre, C. Coulouarn, L. Yon, J.-P. Salier, L. E. Eiden, A. Fournier, H. Vaudry, et al. Microarray and Suppression Subtractive Hybridization Analyses of Gene Expression in Pheochromocytoma Cells Reveal Pleiotropic Effects of Pituitary Adenylate Cyclase-Activating Polypeptide on Cell Proliferation, Survival, and Adhesion Endocrinology, June 1, 2003; 144(6): 2368 - 2379. [Abstract] [Full Text] [PDF]