help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, Y.
Right arrow Articles by Tsang, B. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, Y.
Right arrow Articles by Tsang, B. K.
Endocrinology Vol. 143, No. 7 2732-2740
Copyright © 2002 by The Endocrine Society

REPRODUCTION-DEVELOPMENT

Involvement of Inhibitory Nuclear Factor-{kappa}B (NF{kappa}B)-Independent NF{kappa}B Activation in the Gonadotropic Regulation of X-Linked Inhibitor of Apoptosis Expression during Ovarian Follicular Development in Vitro

Yifang Wang, Simon Chan and Benjamin K. Tsang

Reproductive Biology Unit and Division of Reproductive Medicine, Department of Obstetrics and Gynecology and Cellular and Molecular Medicine, University of Ottawa, Ottawa Health Research Institute, The Ottawa Hospital (Civic Campus), Ottawa, Ontario, Canada K1Y 4E9

Address all correspondence and requests for reprints to: Benjamin K. Tsang, Ph.D., Ottawa Health Research Institute, The Ottawa Hospital (Civic Campus), 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9. E-mail: . btsang{at}ohri.ca

Increased X-linked inhibitor of apoptosis (XIAP) expression and suppressed follicular apoptosis are important determinants in the regulation of follicular development by FSH. The objective of the present study was to examine the role and regulation of nuclear factor-{kappa}B (NF{kappa}B) in the gonadotropic control of granulosa cell XIAP expression and follicular growth in vitro. FSH (100 ng/ml) increased rat granulosa cell XIAP mRNA abundance and protein content. The gonadotropin also induced granulosa cell p65 subunit-containing NF{kappa}B translocation from cytoplasm to nucleus and increased NF{kappa}B-DNA binding activity. Supershift EMSA indicated the FSH-activated NF{kappa}B contained p65 and p50 subunits. Unlike TNF{alpha}, FSH failed to elicit a significant change in granulosa cell phospho- and total-inhibitory NF{kappa}B (I{kappa}B) I{kappa}B contents in vitro and dominant-negative I{kappa}B expression was ineffective in blocking the increase in NF{kappa}B-DNA-binding activity and XIAP protein content induced by the gonadotropin. In contrast, SN50 (a cell permeable inhibitory peptide of NF{kappa}B translocation, 50–200 ng/ml) suppressed FSH-stimulated NF{kappa}B-DNA binding, XIAP expression, and follicular growth. FSH also increased granulosa cell phospho-Akt contents, a response sensitive to the PI-3K inhibitor LY294002 (10 µM). In conclusion, the present studies demonstrate that the FSH-induced XIAP expression is mediated through the NF{kappa}B pathway through activation of phosphatidylinositol 3-kinase rather than the classical I{kappa}B kinase.




This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
H. Wang, J.-Y. Jiang, C. Zhu, C. Peng, and B. K. Tsang
Role and Regulation of Nodal/Activin Receptor-Like Kinase 7 Signaling Pathway in the Control of Ovarian Follicular Atresia
Mol. Endocrinol., October 1, 2006; 20(10): 2469 - 2482.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
S. M Quirk, R. G Cowan, and R. M Harman
The susceptibility of granulosa cells to apoptosis is influenced by oestradiol and the cell cycle.
J. Endocrinol., June 1, 2006; 189(3): 441 - 453.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Tsai-Turton and U. Luderer
Opposing Effects of Glutathione Depletion and Follicle-Stimulating Hormone on Reactive Oxygen Species and Apoptosis in Cultured Preovulatory Rat Follicles
Endocrinology, March 1, 2006; 147(3): 1224 - 1236.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. L. Hamada, K. Nakabayashi, A. Sato, K. Kiyoshi, Y. Takamatsu, J. B. Laoag-Fernandez, N. Ohara, and T. Maruo
Transfection of Antisense Chorionic Gonadotropin {beta} Gene into Choriocarcinoma Cells Suppresses the Cell Proliferation and Induces Apoptosis
J. Clin. Endocrinol. Metab., August 1, 2005; 90(8): 4873 - 4879.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Lee and C. Rivier
Role Played by Hypothalamic Nuclear Factor-{kappa}B in Alcohol-Mediated Activation of the Rat Hypothalamic-Pituitary-Adrenal Axis
Endocrinology, April 1, 2005; 146(4): 2006 - 2014.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J.-Y. Jiang and B. K. Tsang
Optimal Conditions for Successful In Vitro Fertilization and Subsequent Embryonic Development in Sprague-Dawley Rats
Biol Reprod, December 1, 2004; 71(6): 1974 - 1979.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Chu, Y. Nishi, T. Yanase, H. Nawata, and P. J. Fuller
Transrepression of Estrogen Receptor {beta} Signaling by Nuclear Factor-{kappa}B in Ovarian Granulosa Cells
Mol. Endocrinol., August 1, 2004; 18(8): 1919 - 1928.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Fraser, B. M. Leung, X. Yan, H. C. Dan, J. Q. Cheng, and B. K. Tsang
p53 Is a Determinant of X-Linked Inhibitor of Apoptosis Protein/Akt-Mediated Chemoresistance in Human Ovarian Cancer Cells
Cancer Res., November 1, 2003; 63(21): 7081 - 7088.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2002 by The Endocrine Society