This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, Y. Articles by Tsang, B. K. Search for Related Content PubMed PubMed Citation Articles by Wang, Y. Articles by Tsang, B. K.

Involvement of Inhibitory Nuclear Factor-B (NFB)-Independent NFB Activation in the Gonadotropic Regulation of X-Linked Inhibitor of Apoptosis Expression during Ovarian Follicular Development in Vitro

Reproductive Biology Unit and Division of Reproductive Medicine, Department of Obstetrics and Gynecology and Cellular and Molecular Medicine, University of Ottawa, Ottawa Health Research Institute, The Ottawa Hospital (Civic Campus), Ottawa, Ontario, Canada K1Y 4E9

Address all correspondence and requests for reprints to: Benjamin K. Tsang, Ph.D., Ottawa Health Research Institute, The Ottawa Hospital (Civic Campus), 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9. E-mail: . btsang{at}ohri.ca

Increased X-linked inhibitor of apoptosis (XIAP) expression and suppressed follicular apoptosis are important determinants in the regulation of follicular development by FSH. The objective of the present study was to examine the role and regulation of nuclear factor-B (NFB) in the gonadotropic control of granulosa cell XIAP expression and follicular growth in vitro. FSH (100 ng/ml) increased rat granulosa cell XIAP mRNA abundance and protein content. The gonadotropin also induced granulosa cell p65 subunit-containing NFB translocation from cytoplasm to nucleus and increased NFB-DNA binding activity. Supershift EMSA indicated the FSH-activated NFB contained p65 and p50 subunits. Unlike TNF, FSH failed to elicit a significant change in granulosa cell phospho- and total-inhibitory NFB (IB) IB contents in vitro and dominant-negative IB expression was ineffective in blocking the increase in NFB-DNA-binding activity and XIAP protein content induced by the gonadotropin. In contrast, SN50 (a cell permeable inhibitory peptide of NFB translocation, 50–200 ng/ml) suppressed FSH-stimulated NFB-DNA binding, XIAP expression, and follicular growth. FSH also increased granulosa cell phospho-Akt contents, a response sensitive to the PI-3K inhibitor LY294002 (10 µM). In conclusion, the present studies demonstrate that the FSH-induced XIAP expression is mediated through the NFB pathway through activation of phosphatidylinositol 3-kinase rather than the classical IB kinase.

This article has been cited by other articles:

				S. Chu, M. Alexiadis, and P. J. Fuller Proteasome Inhibition by Bortezomib Decreases Proliferation and Increases Apoptosis in Ovarian Granulosa Cell Tumors Reproductive Sciences, April 1, 2009; 16(4): 397 - 407. [Abstract] [PDF]

				H. Wang, J.-Y. Jiang, C. Zhu, C. Peng, and B. K. Tsang Role and Regulation of Nodal/Activin Receptor-Like Kinase 7 Signaling Pathway in the Control of Ovarian Follicular Atresia Mol. Endocrinol., October 1, 2006; 20(10): 2469 - 2482. [Abstract] [Full Text] [PDF]

				S. M Quirk, R. G Cowan, and R. M Harman The susceptibility of granulosa cells to apoptosis is influenced by oestradiol and the cell cycle. J. Endocrinol., June 1, 2006; 189(3): 441 - 453. [Abstract] [Full Text] [PDF]

				M. Tsai-Turton and U. Luderer Opposing Effects of Glutathione Depletion and Follicle-Stimulating Hormone on Reactive Oxygen Species and Apoptosis in Cultured Preovulatory Rat Follicles Endocrinology, March 1, 2006; 147(3): 1224 - 1236. [Abstract] [Full Text] [PDF]

				A. L. Hamada, K. Nakabayashi, A. Sato, K. Kiyoshi, Y. Takamatsu, J. B. Laoag-Fernandez, N. Ohara, and T. Maruo Transfection of Antisense Chorionic Gonadotropin {beta} Gene into Choriocarcinoma Cells Suppresses the Cell Proliferation and Induces Apoptosis J. Clin. Endocrinol. Metab., August 1, 2005; 90(8): 4873 - 4879. [Abstract] [Full Text] [PDF]

				S. Lee and C. Rivier Role Played by Hypothalamic Nuclear Factor-{kappa}B in Alcohol-Mediated Activation of the Rat Hypothalamic-Pituitary-Adrenal Axis Endocrinology, April 1, 2005; 146(4): 2006 - 2014. [Abstract] [Full Text] [PDF]

				J.-Y. Jiang and B. K. Tsang Optimal Conditions for Successful In Vitro Fertilization and Subsequent Embryonic Development in Sprague-Dawley Rats Biol Reprod, December 1, 2004; 71(6): 1974 - 1979. [Abstract] [Full Text] [PDF]

				S. Chu, Y. Nishi, T. Yanase, H. Nawata, and P. J. Fuller Transrepression of Estrogen Receptor {beta} Signaling by Nuclear Factor-{kappa}B in Ovarian Granulosa Cells Mol. Endocrinol., August 1, 2004; 18(8): 1919 - 1928. [Abstract] [Full Text] [PDF]

				M. Fraser, B. M. Leung, X. Yan, H. C. Dan, J. Q. Cheng, and B. K. Tsang p53 Is a Determinant of X-Linked Inhibitor of Apoptosis Protein/Akt-Mediated Chemoresistance in Human Ovarian Cancer Cells Cancer Res., November 1, 2003; 63(21): 7081 - 7088. [Abstract] [Full Text] [PDF]