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Endocrinology Vol. 143, No. 8 2936-2943
Copyright © 2002 by The Endocrine Society

ARTICLE

Expression of Microsomal Prostaglandin E Synthase in Bovine Endometrium: Coexpression with Cyclooxygenase Type 2 and Regulation by Interferon-{tau}

Julie Parent, Pierre Chapdelaine, Jean Sirois and Michel A. Fortier

Unité de Recherche en Ontogénie et Reproduction (J.P., P.C., M.A.F.), Centre Hospitalier Universitaire de Québec, Centre de Recherche en Biologie de la Reproduction, and Département d’Obstétrique et Gynécologie (M.A.F.), Université Laval, Ste-Foy, Québec, Canada G1V 4G2; and Centre de Recherche en Reproduction Animale (J.S.), Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 7C6

Address all correspondence and requests for reprints to: Dr. Michel A. Fortier, Unité de Recherche en Ontogénie et Reproduction, Centre Hostpitalier Universitaire de Québec, Université Laval, 2705 boulevard Laurier, Ste-Foy, Québec, Canada G1V 4G2. E-mail: . mafortier{at}crchul.ulaval.ca

Prostaglandins (PGs) are important regulators of reproductive functions. In ruminants, interferon (IFN)-{tau} is the embryonic signal responsible for recognition of pregnancy. This is effected by a reduction of the production of PGF2{alpha} relative to PGE2. This may be accomplished by a decrease in PGF2{alpha} production, but a stimulation of PGE2 via the PGE synthase might also be involved. The purpose of the present study was to confirm the presence of PGE synthase (PGES) in the bovine endometrium, identify the factors affecting its expression, and compare it with that of cyclooxygenase-2 (COX-2). This was done by Northern blot analysis using primary cultures of bovine epithelial and stromal cells of the endometrium and bovine endometrial cell line. PGES mRNA expression was increased in the presence of lipopolysaccharides, TNF-{alpha}, and IFN-{tau} in stromal cells and IFN-{tau} in epithelial cells. In stromal cells, IFN-{tau} induced a rapid increase of PGES and COX-2 mRNA expression. In bovine endometrial cells, phorbol 12-myristate 13-actetate increased PGES mRNA, COX-2 mRNA and PGE2 production. These results suggest that in endometrial cells, the expression of PGE synthase is correlated with that of COX-2 and is an important enzyme for the production of PGE2. Increasing this production will modulate the PGE2/PGF2{alpha} ratio and contribute to establishment of pregnancy.




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