This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Furlow, J. D. Articles by Kanamori, A. Search for Related Content PubMed PubMed Citation Articles by Furlow, J. D. Articles by Kanamori, A.

The Transcription Factor Basic Transcription Element-Binding Protein 1 Is a Direct Thyroid Hormone Response Gene in the Frog Xenopus laevis

Section of Neurobiology, Physiology, and Behavior, University of California (J.D.F.), Davis, California 95616-8519; and Laboratory of Molecular Genetics, Department of Biology, Nagoya University (A.K.), Chikusa, Nagoya 464-8602, Japan

Address all correspondence and requests for reprints to: J. David Furlow, Ph.D., Section of Neurobiology, Physiology, and Behavior, University of California, One Shields Avenue, Davis, California 95616-8519. E-mail: jdfurlow{at}ucdavis.edu.

Several genes have been identified that are activated or repressed by thyroid hormone in tadpole tissues during metamorphosis of the frog Xenopus laevis. One rapidly and strongly induced gene encodes the Xenopus homolog of basic transcription element-binding protein 1 (xBTEB1), an SP1-related transcription factor. xBTEB1 has similar DNA-binding activity and transcriptional activation properties as mammalian BTEB1. The thyroid hormone-dependent regulation of xBTEB1 was investigated using a modified electrophoretic mobility shift assay to scan genomic DNA for receptor-binding sites. Due to the tetraploid X. laevis genome, xBTEB1 is duplicated, and thyroid hormone regulates both copies. A consensus thyroid hormone response element (TRE) lies far upstream of the transcriptional start site of both genes. The TRE is nested within a 200-bp region of high sequence conservation between these two genes that duplicated millions of years ago. The TRE acts as a strong response element in transfection assays using a heterologous promoter or its native context. Thus, one of the earliest thyroid hormone-induced genes in tadpoles is a transcription factor regulated through an evolutionarily conserved TRE. xBTEB1 is predicted to play an important role in downstream gene regulation leading to the growth and remodeling of tissues at metamorphosis.

This article has been cited by other articles:

				P. Bagamasbad, K. L. Howdeshell, L. M. Sachs, B. A. Demeneix, and R. J. Denver A Role for Basic Transcription Element-binding Protein 1 (BTEB1) in the Autoinduction of Thyroid Hormone Receptor J. Biol. Chem., January 25, 2008; 283(4): 2275 - 2285. [Abstract] [Full Text] [PDF]

				D. R. Buchholz, B. D. Paul, and Y.-B. Shi Gene-specific Changes in Promoter Occupancy by Thyroid Hormone Receptor during Frog Metamorphosis: IMPLICATIONS FOR DEVELOPMENTAL GENE REGULATION J. Biol. Chem., December 16, 2005; 280(50): 41222 - 41228. [Abstract] [Full Text] [PDF]

				L. C. Moeller, A. M. Dumitrescu, and S. Refetoff Cytosolic Action of Thyroid Hormone Leads to Induction of Hypoxia-Inducible Factor-1{alpha} and Glycolytic Genes Mol. Endocrinol., December 1, 2005; 19(12): 2955 - 2963. [Abstract] [Full Text] [PDF]

				L. C. Moeller, A. M. Dumitrescu, R. L. Walker, P. S. Meltzer, and S. Refetoff Thyroid Hormone Responsive Genes in Cultured Human Fibroblasts J. Clin. Endocrinol. Metab., February 1, 2005; 90(2): 936 - 943. [Abstract] [Full Text] [PDF]

				M. S. Malo, W. Zhang, F. Alkhoury, P. Pushpakaran, M. A. Abedrapo, M. Mozumder, E. Fleming, A. Siddique, J. W. Henderson, and R. A. Hodin Thyroid Hormone Positively Regulates the Enterocyte Differentiation Marker Intestinal Alkaline Phosphatase Gene via an Atypical Response Element Mol. Endocrinol., August 1, 2004; 18(8): 1941 - 1962. [Abstract] [Full Text] [PDF]

				J. D. Furlow, H. Y. Yang, M. Hsu, W. Lim, D. J. Ermio, G. Chiellini, and T. S. Scanlan Induction of Larval Tissue Resorption in Xenopus laevis Tadpoles by the Thyroid Hormone Receptor Agonist GC-1 J. Biol. Chem., June 18, 2004; 279(25): 26555 - 26562. [Abstract] [Full Text] [PDF]

				A. Luria and J. D. Furlow Spatiotemporal retinoid-X receptor activation detected in live vertebrate embryos PNAS, June 15, 2004; 101(24): 8987 - 8992. [Abstract] [Full Text] [PDF]

				C. C. Helbing, K. Werry, D. Crump, D. Domanski, N. Veldhoen, and C. M. Bailey Expression Profiles of Novel Thyroid Hormone-Responsive Genes and Proteins in the Tail of Xenopus laevis Tadpoles Undergoing Precocious Metamorphosis Mol. Endocrinol., July 1, 2003; 17(7): 1395 - 1409. [Abstract] [Full Text] [PDF]