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Calmodulin-Dependent Kinase I Regulates Adrenal Cell Expression of Aldosterone Synthase

Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, University of Texas Southwestern Medical Center (J.C.C., B.M.D., S.Y., W.E.R.), Dallas, Texas 75390; and Department of Pharmaco-Biology, University of Calabria (V.P.), 87036 Arcavacata di Rende, Italy

Address all correspondence and requests for reprints to: William E. Rainey, Ph.D., Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, University of Texas, Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9032. E-mail: william.rainey{at}utsouthwestern.edu.

Aldosterone synthase (CYP11B2) is expressed in the adrenal glomerulosa and controls the capacity of the adrenal glomerulosa to produce aldosterone. Herein, human NCI-H295R (H295R) adrenocortical cells were used to define the calcium-dependent mechanisms regulating CYP11B2 gene transcription using reporter constructs containing CYP11B2 gene 5'-flanking DNA. Treatment of H295R cells with calcium/calmodulin-dependent protein kinase (CaMK) inhibitor (KN93) or calmodulin inhibitor (calmidazolium) blocked angiotensin II and potassium (K⁺) stimulation of CYP11B2 reporter gene expression. To determine which CaMK regulates CYP11B2, vectors containing the complete coding sequences for CaMKI, CaMKII, and CaMKIV were transfected with the CYP11B2 reporter construct. CaMKI augmented reporter expression when cellular calcium was elevated by ionomycin, whereas CaMKIV had a small effect, and CaMKII had no effect. To further study the role of CaMKs, constitutively active forms of CaMKI (CaMKI-295), II (CaMKII-290), and IV (CaMKIV-313) were transfected with CYP11B2 reporter constructs. CaMKI-295 and, to a lesser degree, CaMKIV-313 were able to stimulated reporter activity. Mutational analysis of the 5'-flanking region of CYP11B2 revealed that a cAMP regulatory element (-71/-64) was necessary for CaMKI induction of reporter gene activity. CaMKI expression was shown in adrenal cortex and H295R cells using immunohistochemistry and Western and Northern analyses. These findings suggest that CaMKI is involved in angiotensin II and K⁺ stimulation of CYP11B2 transcription and, therefore, the capacity of the adrenal to produce aldosterone.

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