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Endocrinology Vol. 144, No. 3 754-759
Copyright © 2003 by The Endocrine Society

ARTICLE

The Role of Ghrelin and Growth Hormone Secretagogues Receptor on Rat Adipogenesis

Kichoon Choi, Sang-Gun Roh, Yeon-Hee Hong, Yogendra B. Shrestha, Daisuke Hishikawa, Chen Chen, Masayasu Kojima, Kenji Kangawa and Shin-Ichi Sasaki

Department of Food Resource Science (K.C., S.-G.R., Y.-H.H., Y.B.S., D.H., S.S.), Faculty of Agriculture, Shinshu University, Nagano-ken 399-4598, Japan; Endocrine Cell Biology (C.C.), Prince Henry’s Institute of Medical Research, Clayton 3168, Australia; Molecular Genetics (M.K.), Institute of Life Science, Kurume University, Aikawamachi 2432-3, Kurume, Fukuoka 839-0861, Japan; and Department of Biochemistry (K.K.), National Cardiovascular Center Research Institute, Fujishirodai, Suita, Osaka 565-8565, Japan

Address all correspondence and requests for reprints to: Dr. Sang-gun Roh, Department of Food Resource Science Faculty of Agriculture, Shinshu University Nagano-ken 399-4598, Japan. E-mail: sangroh{at}gipmc.shinshu-u.ac.jp.

Recent research progress indicates a close link between ghrelin, a natural ligand of GH secretagogues receptor (GHS-R), and both the metabolic balance and body composition. To clarify the involvement of ghrelin and GHS-R in the process of adipogenesis, we measured the expression of GHS-R and peroxisome proliferator-activated receptor {gamma} 2 (PPAR-{gamma}2) mRNA in rat adipocytes using semiquantitative RT-PCR methods. The levels of GHS-R mRNA increased by up to 4-fold in adipose tissue from epididymal and parametrial regions as the rat aged from 4–20 wk and were significantly elevated during the differentiation of preadipocytes in vitro. Ghrelin (10-8 M for 10 d) stimulated the activity of glycerol-3-phosphate dehydrogenase and the differentiation of rat preadipocytes in vitro. Ghrelin treatment also significantly increased the levels of PPAR-{gamma}2 mRNA in primary cultured rat differentiated adipocytes. In addition, isoproterenol (10-8 M, 40 min)-stimulated lipolysis was significantly reduced by simultaneous ghrelin treatment in a dose-dependent manner in vitro. In conclusion, the expression of GHS-R in rat adipocytes increases with the age and during adipogenesis. Ghrelin in vitro stimulates the differentiation of preadipocytes and antagonizes lipolysis. Ghrelin may therefore play an important role in the process of adipogenesis in rats.




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