| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Reproductive Endocrine Unit, Massachusetts General Hospital, Boston, Massachusetts 02114
Address all correspondence and requests for reprints to: Alan Schneyer, Reproductive Endocrine Unit, BHX-5, Massachusetts General Hospital, Boston, Massachusetts 02114. E-mail: schneyer.alan{at}mgh.harvard.edu.
Modulation of activin and other TGFß superfamily signaling is the primary mechanism of action for both follistatin (FS) and FS-like 3 (FSTL-3). However, most studies of these ligands use activin A due to its wide availability. We have now tested the ability of FS288 and FSTL-3 to bind and neutralize activin B relative to activin A. Activin B bound to both FS and FSTL-3 at a potency approximately 10-fold lower than that of activin A. Moreover, whereas both activins had similar biological activity in 293 cell reporter assays, FS and FSTL-3 were approximately 3-fold more effective in neutralizing activin A relative to activin B. These results suggest that neutralization of activins A and B by FS and FSTL-3 are not identical, so that the relative activity of each activin in tissues where both are produced, such as in the ovary, could be quite different. In addition, biological systems that use primarily activin B, but which have been examined in vitro using activin A, may need to be reevaluated to determine the actual physiologic roles of FS or FSTL-3.
This article has been cited by other articles:
 |
|
 |
|
  C. L. Beites, P. L. W. Hollenbeck, J. Kim, R. Lovell-Badge, A. D. Lander, and A. L. Calof Follistatin modulates a BMP autoregulatory loop to control the size and patterning of sensory domains in the developing tongue Development, July 1, 2009; 136(13): 2187 - 2197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Stoikos, C. A. Harrison, L. A. Salamonsen, and E. Dimitriadis A distinct cohort of the TGF{beta} superfamily members expressed in human endometrium regulate decidualization Hum. Reprod., June 1, 2008; 23(6): 1447 - 1456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-Y. Lin, R. G. Craythorn, A. E. O'Connor, M. M. Matzuk, J. E. Girling, J. R. Morrison, and D. M. de Kretser Female Infertility and Disrupted Angiogenesis Are Actions of Specific Follistatin Isoforms Mol. Endocrinol., February 1, 2008; 22(2): 415 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kashiwagi, C. M. DiGirolamo, Y. Kanda, Y. Niikura, C. T. Esmon, T. R. Hansen, T. Shioda, and J. K. Pru The Postimplantation Embryo Differentially Regulates Endometrial Gene Expression and Decidualization Endocrinology, September 1, 2007; 148(9): 4173 - 4184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Harrison, K. L. Chan, and D. M. Robertson Activin-A Binds Follistatin and Type II Receptors through Overlapping Binding Sites: Generation of Mutants with Isolated Binding Activities Endocrinology, June 1, 2006; 147(6): 2744 - 2753. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-G. Chen, Q. Wang, S.-L. Lin, C. D. Chang, J. Chung, and S.-Y. Ying Activin Signaling and Its Role in Regulation of Cell Proliferation, Apoptosis, and Carcinogenesis. Experimental Biology and Medicine, May 1, 2006; 231(5): 534 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Kumar Too Many Follistatins: Racing Inside and Getting Out of the Cell Endocrinology, December 1, 2005; 146(12): 5048 - 5051. [Full Text] [PDF]
|
|
|
|
|
|
Y. Sidis, A. L. Schneyer, and H. T. Keutmann Heparin and Activin-Binding Determinants in Follistatin and FSTL3 Endocrinology, January 1, 2005; 146(1): 130 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. del Re, Y. Sidis, D. A. Fabrizio, H. Y. Lin, and A. Schneyer Reconstitution and Analysis of Soluble Inhibin and Activin Receptor Complexes in a Cell-free System J. Biol. Chem., December 17, 2004; 279(51): 53126 - 53135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Schneyer, Q. Wang, Y. Sidis, and P. M. Sluss Differential Distribution of Follistatin Isoforms: Application of a New FS315-Specific Immunoassay J. Clin. Endocrinol. Metab., October 1, 2004; 89(10): 5067 - 5075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. del Re, J. L. Babitt, A. Pirani, A. L. Schneyer, and H. Y. Lin In the Absence of Type III Receptor, the Transforming Growth Factor (TGF)-{beta} Type II-B Receptor Requires the Type I Receptor to Bind TGF-{beta}2 J. Biol. Chem., May 21, 2004; 279(21): 22765 - 22772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Bertani, C. D. Gladney, R. K. Johnson, and D. Pomp Evaluation of gene expression in pigs selected for enhanced reproduction using differential display PCR: II. Anterior pituitary J Anim Sci, January 1, 2004; 82(1): 32 - 40. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
