help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haisenleder, D. J.
Right arrow Articles by Shupnik, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haisenleder, D. J.
Right arrow Articles by Shupnik, M. A.
Endocrinology Vol. 144, No. 6 2409-2416
Copyright © 2003 by The Endocrine Society

The Calcium Component of Gonadotropin-Releasing Hormone-Stimulated Luteinizing Hormone Subunit Gene Transcription Is Mediated by Calcium/Calmodulin-Dependent Protein Kinase Type II

Daniel J. Haisenleder, Heather A. Ferris and Margaret A. Shupnik

Division of Endocrinology (D.J.H., M.A.S.), Department of Medicine and Department of Molecular Physiology and Biological Physics (H.A.F., M.A.S.), University of Virginia Health Science Center, Charlottesville, Virginia 22908

Address all correspondence and requests for reprints to: Division of Endocrinology, Department of Medicine, and +Department of Molecular Physiology and Biological Physics, University of Virginia Health Science Center, Charlottesville, Virginia 22908. E-mail: djh2q{at}virginia.edu.

Calcium influx plays a critical role in GnRH regulation of rat LH subunit gene transcription, but the site(s) of action are undefined. We investigated the potential of GnRH acting through calcium to activate calcium/calmodulin-dependent protein kinase type II (Ca/CaMK II) in mouse gonadotrope-derived LßT2 cells. GnRH stimulated Ca/CaMK II ß subunit activity 3-fold 2 min after treatment and returned to control values by 45 min. The Ca/CaMK II response to GnRH was blocked by administration of the Ca/CaMK II-specific inhibitor, KN-93. The calcium channel activator Bay K 8644 stimulated a 3-fold increase in Ca/CaMK II activity, similar to GnRH. Blocking calcium influx with nimodipine or depleting intracellular calcium storage pools with thapsigargin each resulted in a partial suppression of GnRH-induced activation of Ca/CaMK II, and in combination, completely suppressed the Ca/CaMK II response to GnRH. KN-93 and nimodipine also suppressed {alpha}-subunit and LHß promoter responses to GnRH by 40–60%. LHß promoter constructs containing either proximal or proximal and distal GnRH-responsive regions were sensitive to inhibition. These data show for the first time that Ca/CaMK II activation plays an important role in the transmission of GnRH signals from the plasma membrane to the LH subunit genes.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
I. P. Sari, A. Rao, J. T. Smith, A. J. Tilbrook, and I. J. Clarke
Effect of RF-Amide-Related Peptide-3 on Luteinizing Hormone and Follicle-Stimulating Hormone Synthesis and Secretion in Ovine Pituitary Gonadotropes
Endocrinology, December 1, 2009; 150(12): 5549 - 5556.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Chen, B.-S. An, L. Cheng, G. L. Hammond, and P. C. K. Leung
Gonadotropin-Releasing Hormone-Mediated Phosphorylation of Estrogen Receptor-{alpha} Contributes to fosB Expression in Mouse Gonadotrophs
Endocrinology, October 1, 2009; 150(10): 4583 - 4593.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
C. M. Grafer, R. Thomas, L. Lambrakos, I. Montoya, S. White, and L. M. Halvorson
GnRH Stimulates Expression of PACAP in the Pituitary Gonadotropes via Both the PKA and PKC Signaling Systems
Mol. Endocrinol., July 1, 2009; 23(7): 1022 - 1032.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
H. E. Walsh and M. A. Shupnik
Proteasome Regulation of Dynamic Transcription Factor Occupancy on the GnRH-Stimulated Luteinizing Hormone {beta}-Subunit Promoter
Mol. Endocrinol., February 1, 2009; 23(2): 237 - 250.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. L. Burger, D. J. Haisenleder, K. W. Aylor, and J. C. Marshall
Regulation of Intracellular Signaling Cascades by GNRH Pulse Frequency in the Rat Pituitary: Roles for CaMK II, ERK, and JNK Activation
Biol Reprod, November 1, 2008; 79(5): 947 - 953.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. Xie, K. H. Allen, A. Marguet, K. A. Berghorn, S. P. Bliss, A. M. Navratil, J. L. Guan, and M. S. Roberson
Analysis of the Calcium-Dependent Regulation of Proline-Rich Tyrosine Kinase 2 by Gonadotropin-Releasing Hormone
Mol. Endocrinol., October 1, 2008; 22(10): 2322 - 2335.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. J. Haisenleder, L. L. Burger, H. E. Walsh, J. Stevens, K. W. Aylor, M. A. Shupnik, and J. C. Marshall
Pulsatile Gonadotropin-Releasing Hormone Stimulation of Gonadotropin Subunit Transcription in Rat Pituitaries: Evidence for the Involvement of Jun N-Terminal Kinase But Not p38
Endocrinology, January 1, 2008; 149(1): 139 - 145.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. A Ferris, H. E Walsh, J. Stevens, P. C Fallest, and M. A Shupnik
Luteinizing Hormone Beta Promoter Stimulation by Adenylyl Cyclase and Cooperation with Gonadotropin-Releasing Hormone 1 in Transgenic Mice and LBetaT2 Cells
Biol Reprod, December 1, 2007; 77(6): 1073 - 1080.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Lim, M. Luo, M. Koh, M. Yang, M. N. bin Abdul Kadir, J. H. Tan, Z. Ye, W. Wang, and P. Melamed
Distinct Mechanisms Involving Diverse Histone Deacetylases Repress Expression of the Two Gonadotropin {beta}-Subunit Genes in Immature Gonadotropes, and Their Actions Are Overcome by Gonadotropin-Releasing Hormone
Mol. Cell. Biol., June 1, 2007; 27(11): 4105 - 4120.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. A. Ferris and M. A. Shupnik
Mechanisms for Pulsatile Regulation of the Gonadotropin Subunit Genes by GNRH1
Biol Reprod, June 1, 2006; 74(6): 993 - 998.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. S. Roberson, S. P. Bliss, J. Xie, A. M. Navratil, T. A. Farmerie, M. W. Wolfe, and C. M. Clay
Gonadotropin-Releasing Hormone Induction of Extracellular-Signal Regulated Kinase Is Blocked by Inhibition of Calmodulin
Mol. Endocrinol., September 1, 2005; 19(9): 2412 - 2423.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
F. Liu, M. S. Ruiz, D. A. Austin, and N. J. G. Webster
Constitutively Active Gq Impairs Gonadotropin-Releasing Hormone-Induced Intracellular Signaling and Luteinizing Hormone Secretion in L{beta}T2 Cells
Mol. Endocrinol., August 1, 2005; 19(8): 2074 - 2085.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D.J. Haisenleder, L.L. Burger, K.W. Aylor, A.C. Dalkin, H.E. Walsh, M.A. Shupnik, and J.C. Marshall
Testosterone Stimulates Follicle-Stimulating Hormone {beta} Transcription via Activation of Extracellular Signal-Regulated Kinase: Evidence in Rat Pituitary Cells
Biol Reprod, March 1, 2005; 72(3): 523 - 529.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
L L Burger, D J Haisenleder, A C Dalkin, and J C Marshall
Regulation of gonadotropin subunit gene transcription
J. Mol. Endocrinol., December 1, 2004; 33(3): 559 - 584.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2003 by The Endocrine Society