help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology, doi:10.1210/en.2002-221057
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, S.-K.
Right arrow Articles by Lorenzo, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, S.-K.
Right arrow Articles by Lorenzo, J. A.
Endocrinology Vol. 144, No. 8 3524-3531
Copyright © 2003 by The Endocrine Society

Interleukin-7 Is a Direct Inhibitor of in Vitro Osteoclastogenesis

Sun-Kyeong Lee, Judith F. Kalinowski, Sandra L. Jastrzebski, Lynn Puddington and Joseph A. Lorenzo

Division of Endocrinology (S.-K.L., J.F.K., S.L.J., J.A.L.) and Division of Immunology (L.P.), Department of Medicine, The University of Connecticut Health Center, Farmington, Connecticut 06030

Address all correspondence and requests for reprints to: Dr. Sun-Kyeong Lee, Division of Endocrinology, Department of Medicine, MC-1850, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030-1850. E-mail: slee{at}neuron.uchc.edu.

We examined the direct effects of IL-7 on osteoclastogenesis in murine bone marrow cultures, using cells from wild-type and IL-7- and IL-7 receptor (IL-7R)-deficient mice. IL-7 inhibited osteoclast-like cells (OCL) formation in macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor {kappa}B ligand (RANKL)-stimulated (both at 30 ng/ml) murine bone marrow cultures. Significant inhibitory effects were seen at 1 ng/ml (57%) and 10 ng/ml (86%). IL-7 also inhibited (P < 0.05) OCL formation in bone marrow cultures that were stimulated with vitamin D3 (10-8 M, 60%), bovine PTH (bPTH) (100 ng/ml, 54%), or RANKL alone (30 ng/ml, 50%). IL-7 (10 ng/ml) increased expression of the B lymphocyte marker B220 from 40–86% of total nonadherent cells in cultures treated with M-CSF and RANKL. Bone marrow cells from IL-7-deficient [IL-7 knockout (KO)] mice showed a significant (P < 0.05) increase in tartrate-resistant acid phosphatase(+) OCL numbers in cultures that were stimulated with vitamin D3 (136 ± 13.3%), bPTH (196 ± 18.8%), or M-CSF and RANKL (160 ± 7.2%). In contrast, in vitro osteoclast formation in bone marrow from IL-7R-deficient (IL-7R KO) mice showed a significant decrease in tartrate-resistant acid phosphatase(+) OCL numbers in cultures that were stimulated with vitamin D3, PTH, RANKL, or M-CSF and RANKL. These results demonstrate that there are differences in the mechanisms regulating OCL formation between IL-7 KO and IL-7R KO cells. It seems that IL-7 is a direct inhibitor of OCL formation in vitro, based on results of adding IL-7 to wild-type cultures and the responses of IL-7 KO cells. It is unknown why IL-7R KO cells behave differently from IL-7 KO cells in vitro. However, it is possible that additional cytokines interact with IL-7R and that loss of these signals contributes to the responses of IL-7R KO cells. Alternatively, IL-7 may interact with multiple receptors.




This article has been cited by other articles:


Home page
 Endocr. Rev.Home page
J. Lorenzo, M. Horowitz, and Y. Choi
Osteoimmunology: Interactions of the Bone and Immune System
Endocr. Rev., June 1, 2008; 29(4): 403 - 440.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S.-J. Lee, R. J. Rossi, S.-K. Lee, M. Croft, B. S. Kwon, R. S. Mittler, and A. T. Vella
CD134 Costimulation Couples the CD137 Pathway to Induce Production of Supereffector CD8 T Cells That Become IL-7 Dependent
J. Immunol., August 15, 2007; 179(4): 2203 - 2214.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
U.H. Lerner
Inflammation-induced Bone Remodeling in Periodontal Disease and the Influence of Post-menopausal Osteoporosis
Journal of Dental Research, July 1, 2006; 85(7): 596 - 607.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
Y. Fujimura, H. Hotokezaka, N. Ohara, M. Naito, E. Sakai, M. Yoshimura, Y. Narita, H. Kitaura, N. Yoshida, and K. Nakayama
The Hemoglobin Receptor Protein of Porphyromonas gingivalis Inhibits Receptor Activator NF-{kappa}B Ligand-Induced Osteoclastogenesis from Bone Marrow Macrophages.
Infect. Immun., May 1, 2006; 74(5): 2544 - 2551.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. C. Horowitz, Y. Xi, D. L. Pflugh, D. G. T. Hesslein, D. G. Schatz, J. A. Lorenzo, and A. L. M. Bothwell
Pax5-Deficient Mice Exhibit Early Onset Osteopenia with Increased Osteoclast Progenitors
J. Immunol., December 1, 2004; 173(11): 6583 - 6591.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S.-I. Hayashi, T. Yamada, M. Tsuneto, T. Yamane, M. Takahashi, L. D. Shultz, and H. Yamazaki
Distinct Osteoclast Precursors in the Bone Marrow and Extramedullary Organs Characterized by Responsiveness to Toll-Like Receptor Ligands and TNF-{alpha}
J. Immunol., November 15, 2003; 171(10): 5130 - 5139.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2003 by The Endocrine Society