help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology, doi:10.1210/en.2003-0314
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Song, Y.
Right arrow Articles by Christakos, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Song, Y.
Right arrow Articles by Christakos, S.
Endocrinology Vol. 144, No. 9 3885-3894
Copyright © 2003 by The Endocrine Society

Calcium Transporter 1 and Epithelial Calcium Channel Messenger Ribonucleic Acid Are Differentially Regulated by 1,25 Dihydroxyvitamin D3 in the Intestine and Kidney of Mice

Yurong Song, Xiaorong Peng, Angela Porta, Hitomi Takanaga, Ji-Bin Peng, Matthias A. Hediger, James C. Fleet and Sylvia Christakos

Interdepartmental Nutrition Program (Y.S., J.C.F.), Purdue University, West Lafayette, Indiana 47907; Renal Division and Membrane Biology Program (H.T., J.-B.P., M.A.H.), Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts 02115; and Department of Biochemistry and Molecular Biology (X.P., A.P., S.C.), University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark, New Jersey 07103

Address all correspondence and requests for reprints to: Dr. Sylvia Christakos, Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, 185 South Orange Avenue, MSB Room E665, Newark, New Jersey 07103. E-mail: christak{at}umdnj.edu; or James C. Fleet, Ph.D., 700 West State Street, Purdue University, West Lafayette, Indiana 47907-2059. E-mail: fleetj{at}cfs.purdue.edu.

We examined the expression of calcium transporter 1 (CaT1) and epithelial calcium channel (ECaC) mRNA in the duodenum and kidney of mice. Intestinal CaT1 mRNA level increased 30-fold at weaning, coincident with the induction of calbindin-D9k expression. In contrast, renal CaT1 and ECaC mRNA expression was equal until weaning when ECaC mRNA is induced and CaT1 mRNA levels fall 70%. Long- and short-term adaptation to changes in dietary calcium (Ca) level and 1,25 dihydroxyvitamin D3 [1,25(OH)2D3] injection strongly regulated duodenal calbindin D9k and CaT1 mRNA. Following a single dose of 1,25(OH)2D3, induction of CaT1 mRNA occurred rapidly (within 3 h, peak at 6 h of 9.6 ± 0.8-fold) and preceded the induction of intestinal Ca absorption (significantly increased at 6 h, peak at 9 h). Neither renal CaT1 nor ECaC mRNA were strongly regulated by dietary calcium level or 1,25(OH)2D3 injection. Our data indicate that CaT1 and ECaC mRNA levels are differentially regulated by 1,25(OH)2D3 in kidney and intestine and that there may be a specialized role for CaT1 in kidney in fetal and neonatal development. The rapid induction of intestinal CaT1 mRNA expression by 1,25(OH)2D3, and the marked induction at weaning, suggest that CaT1 is critical for 1,25(OH)2D3-mediated intestinal Ca absorption.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
G. D. Kutuzova, F. Sundersingh, J. Vaughan, B. P. Tadi, S. E. Ansay, S. Christakos, and H. F. DeLuca
TRPV6 is not required for 1{alpha},25-dihydroxyvitamin D3-induced intestinal calcium absorption in vivo
PNAS, December 16, 2008; 105(50): 19655 - 19659.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. S. Benn, D. Ajibade, A. Porta, P. Dhawan, M. Hediger, J.-B. Peng, Y. Jiang, G. T. Oh, E.-B. Jeung, L. Lieben, et al.
Active Intestinal Calcium Transport in the Absence of Transient Receptor Potential Vanilloid Type 6 and Calbindin-D9k
Endocrinology, June 1, 2008; 149(6): 3196 - 3205.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
J. C. Fleet, C. Gliniak, Z. Zhang, Y. Xue, K. B. Smith, R. McCreedy, and S. A. Adedokun
Serum Metabolite Profiles and Target Tissue Gene Expression Define the Effect of Cholecalciferol Intake on Calcium Metabolism in Rats and Mice
J. Nutr., June 1, 2008; 138(6): 1114 - 1120.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
M. Ishizawa, M. Matsunawa, R. Adachi, S. Uno, K. Ikeda, H. Masuno, M. Shimizu, K.-i. Iwasaki, S. Yamada, and M. Makishima
Lithocholic acid derivatives act as selective vitamin D receptor modulators without inducing hypercalcemia
J. Lipid Res., April 1, 2008; 49(4): 763 - 772.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
K. A. Bolanz, M. A. Hediger, and C. P. Landowski
The role of TRPV6 in breast carcinogenesis
Mol. Cancer Ther., February 1, 2008; 7(2): 271 - 279.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
M. J. Rowling, C. Gliniak, J. Welsh, and J. C. Fleet
High Dietary Vitamin D Prevents Hypocalcemia and Osteomalacia in CYP27B1 Knockout Mice
J. Nutr., December 1, 2007; 137(12): 2608 - 2615.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. B. Meyer, L. A. Zella, R. D. Nerenz, and J. W. Pike
Characterizing Early Events Associated with the Activation of Target Genes by 1,25-Dihydroxyvitamin D3 in Mouse Kidney and Intestine in Vivo
J. Biol. Chem., August 3, 2007; 282(31): 22344 - 22352.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G.-S. Lee and E.-B. Jeung
Uterine TRPV6 expression during the estrous cycle and pregnancy in a mouse model
Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E132 - E138.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
Y. Gao and M. G. Wheatly
Molecular characterization of an epithelial Ca2+ channel-like gene from crayfish Procambarus clarkii
J. Exp. Biol., May 15, 2007; 210(10): 1813 - 1824.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y. Song and J. C. Fleet
Intestinal Resistance to 1,25 Dihydroxyvitamin D in Mice Heterozygous for the Vitamin D Receptor Knockout Allele
Endocrinology, March 1, 2007; 148(3): 1396 - 1402.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
Y. Zhang, W.-P. Lai, C.-F. Wu, M. J. Favus, P.-C. Leung, and M.-S. Wong
Ovariectomy worsens secondary hyperparathyroidism in mature rats during low-Ca diet
Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E723 - E731.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Shahsavarani and S. F. Perry
Hormonal and environmental regulation of epithelial calcium channel in gill of rainbow trout (Oncorhynchus mykiss)
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2006; 291(5): R1490 - R1498.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
N. Charoenphandhu, K. Tudpor, N. Pulsook, and N. Krishnamra
Chronic metabolic acidosis stimulated transcellular and solvent drag-induced calcium transport in the duodenum of female rats
Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G446 - G455.
[Abstract] [Full Text] [PDF]

Home page
 The Annals of PharmacotherapyHome page
J. Q Hudson
Secondary Hyperparathyroidism in Chronic Kidney Disease: Focus on Clinical Consequences and Vitamin D Therapies
Ann. Pharmacother., September 1, 2006; 40(9): 1584 - 1593.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H.-J. Kim, G.-S. Lee, Y.-K. Ji, K.-C. Choi, and E.-B. Jeung
Differential expression of uterine calcium transporter 1 and plasma membrane Ca2+ ATPase 1b during rat estrous cycle.
Am J Physiol Endocrinol Metab, August 1, 2006; 291(2): E234 - E241.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. B. Meyer, M. Watanuki, S. Kim, N. K. Shevde, and J. W. Pike
The Human Transient Receptor Potential Vanilloid Type 6 Distal Promoter Contains Multiple Vitamin D Receptor Binding Sites that Mediate Activation by 1,25-Dihydroxyvitamin D3 in Intestinal Cells
Mol. Endocrinol., June 1, 2006; 20(6): 1447 - 1461.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
A. Shahsavarani, B. McNeill, F. Galvez, C. M. Wood, G. G. Goss, P.-P. Hwang, and S. F. Perry
Characterization of a branchial epithelial calcium channel (ECaC) in freshwater rainbow trout (Oncorhynchus mykiss)
J. Exp. Biol., May 15, 2006; 209(10): 1928 - 1943.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
K. Tudpor, N. Charoenphandhu, W. Saengamnart, and N. Krishnamra
Long-Term Prolactin Exposure Differentially Stimulated the Transcellular and Solvent Drag-Induced Calcium Transport in the Duodenum of Ovariectomized Rats
Experimental Biology and Medicine, December 1, 2005; 230(11): 836 - 844.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. S. Kovacs, M. L. Woodland, N. J. Fudge, and J. K. Friel
The vitamin D receptor is not required for fetal mineral homeostasis or for the regulation of placental calcium transfer in mice
Am J Physiol Endocrinol Metab, July 1, 2005; 289(1): E133 - E144.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. Dhawan, X. Peng, A. L. M. Sutton, P. N. MacDonald, C. M. Croniger, C. Trautwein, M. Centrella, T. L. McCarthy, and S. Christakos
Functional Cooperation between CCAAT/Enhancer-Binding Proteins and the Vitamin D Receptor in Regulation of 25-Hydroxyvitamin D3 24-Hydroxylase
Mol. Cell. Biol., January 1, 2005; 25(1): 472 - 487.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. G. J. Hoenderop, B. Nilius, and R. J. M. Bindels
Calcium Absorption Across Epithelia
Physiol Rev, January 1, 2005; 85(1): 373 - 422.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. G.J. Hoenderop and R. J.M. Bindels
Epithelial Ca2+ and Mg2+ Channels in Health and Disease
J. Am. Soc. Nephrol., January 1, 2005; 16(1): 15 - 26.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Zheng, Y. Xie, G. Li, J. Kong, J. Q. Feng, and Y. C. Li
Critical Role of Calbindin-D28k in Calcium Homeostasis Revealed by Mice Lacking Both Vitamin D Receptor and Calbindin-D28k
J. Biol. Chem., December 10, 2004; 279(50): 52406 - 52413.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Wang, A. Klopot, J.-N. Freund, L. N. Dowling, S. D. Krasinski, and J. C. Fleet
Control of differentiation-induced calbindin-D9k gene expression in Caco-2 cells by cdx-2 and HNF-1{alpha}
Am J Physiol Gastrointest Liver Physiol, November 1, 2004; 287(5): G943 - G953.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
Y. Song and J. C. Fleet
1,25 Dihydroxycholecalciferol-Mediated Calcium Absorption and Gene Expression Are Higher in Female than in Male Mice
J. Nutr., August 1, 2004; 134(8): 1857 - 1861.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2003 by The Endocrine Society