This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arosh, J. A. Articles by Fortier, M. A. Search for Related Content PubMed PubMed Citation Articles by Arosh, J. A. Articles by Fortier, M. A.

Temporal and Tissue-Specific Expression of Prostaglandin Receptors EP2, EP3, EP4, FP, and Cyclooxygenases 1 and 2 in Uterus and Fetal Membranes during Bovine Pregnancy

Unité d’Ontogénie & Reproduction, Centre de Recherche du Centre Hospitalier de l’Université Laval, Ste-Foy, Québec, Canada G1V 4G2; and Centre de Recherche en Biologie de la Reproduction and Département d’Obstétrique et Gynécologie, Université Laval, Ste-Foy, Québec, Canada GIV 4G2

Address all correspondence and requests for reprints to: Dr. Michel A. Fortier, Ph.D., Unité D’Ontogénie & Reproduction, Bloc T1-49, Centre de Recherche du CHUL, 2705 Boulevard Laurier, Ste-Foy, Québec, Canada G1V 4G2. E-mail: mafortier{at}crchul.ulaval.ca.

Uteroplacental prostaglandins (PGs) play pivotal roles in maintenance and /or termination of pregnancy in mammals. Regulation of PG biosynthetic and signaling mechanisms in uteroplacental tissues during maintenance of pregnancy is largely unknown. In the present study, we have characterized the expression of PGE₂ receptors (EP2, EP3, EP4), PGF₂ receptor (FP), and cyclooxygenase (COX) types 1 and 2 in placentome caruncle (CAR), intercaruncle, and fetal membrane tissues during pregnancy in cattle. Pregnant bovine uteri were collected and classified into six groups covering the entire gestational length. The levels of expression of EP2, EP3, and FP mRNAs differ depending on tissues and days of gestation (days < 50 to > 250). EP4 mRNA was undetectable in all the tissues studied. The expression levels of PG receptor mRNAs were as follows: placentome CAR FP > EP2 >EP3, intercaruncle EP2 > EP3 FP, and fetal membranes EP3 EP2 >> FP. EP2 and EP3 expressions were modulated in uteroplacental tissues, depending on days of pregnancy, whereas FP was uniformly expressed. COX-1 mRNA and protein were constitutively expressed, whereas COX-2 was highly modulated in uteroplacental tissues throughout pregnancy. Immunohistochemistry showed that EP2 and COX-2 proteins were colocalized in most cell types of placentome CAR, endometrium, and myometrium. Our study indicates that EP2 is the primary cAMP-generating PGE₂ receptor expressed in uteroplacental tissues during bovine pregnancy. Temporal and tissue-specific expression of PGE₂ and PGF₂ receptors and COX-1 and -2 at the maternal-fetal interface suggests a selective and distinctive role for PGE₂ and PGF₂ in uterine activities during pregnancy in bovine.

This article has been cited by other articles:

				S. P Kodithuwakku, A. Miyamoto, and M. P B Wijayagunawardane Spermatozoa stimulate prostaglandin synthesis and secretion in bovine oviductal epithelial cells Reproduction, June 1, 2007; 133(6): 1087 - 1094. [Abstract] [Full Text] [PDF]

				D. B. Hardy, B. A. Janowski, D. R. Corey, and C. R. Mendelson Progesterone Receptor Plays a Major Antiinflammatory Role in Human Myometrial Cells by Antagonism of Nuclear Factor-{kappa}B Activation of Cyclooxygenase 2 Expression Mol. Endocrinol., November 1, 2006; 20(11): 2724 - 2733. [Abstract] [Full Text] [PDF]

				P. L. Grigsby, S. R. Sooranna, B. Adu-Amankwa, B. Pitzer, D. E. Brockman, M. R. Johnson, and L. Myatt Regional Expression of Prostaglandin E2 and F2alpha Receptors in Human Myometrium, Amnion, and Choriodecidua with Advancing Gestation and Labor Biol Reprod, August 1, 2006; 75(2): 297 - 305. [Abstract] [Full Text] [PDF]

				P. Chapdelaine, J. Kang, S. Boucher-Kovalik, N. Caron, J. P. Tremblay, and M. A. Fortier Decidualization and maintenance of a functional prostaglandin system in human endometrial cell lines following transformation with SV40 large T antigen Mol. Hum. Reprod., May 1, 2006; 12(5): 309 - 319. [Abstract] [Full Text] [PDF]

				B. Doring, O. Shynlova, P. Tsui, D. Eckardt, U. Janssen-Bienhold, F. Hofmann, S. Feil, R. Feil, S. J. Lye, and K. Willecke Ablation of connexin43 in uterine smooth muscle cells of the mouse causes delayed parturition J. Cell Sci., May 1, 2006; 119(9): 1715 - 1722. [Abstract] [Full Text] [PDF]

				D. M. Slater, S. Astle, N. Woodcock, J. E. Chivers, N. C.J. de Wit, S. Thornton, M. Vatish, and R. Newton Anti-inflammatory and relaxatory effects of prostaglandin E2 in myometrial smooth muscle Mol. Hum. Reprod., February 1, 2006; 12(2): 89 - 97. [Abstract] [Full Text] [PDF]

				S.K. Banu, J.A. Arosh, P. Chapdelaine, and M.A. Fortier Expression of Prostaglandin Transporter in the Bovine Uterus and Fetal Membranes During Pregnancy Biol Reprod, August 1, 2005; 73(2): 230 - 236. [Abstract] [Full Text] [PDF]

				J. Kang, P. Chapdelaine, J. Parent, E. Madore, P. Y. Laberge, and M. A. Fortier Expression of Human Prostaglandin Transporter in the Human Endometrium across the Menstrual Cycle J. Clin. Endocrinol. Metab., April 1, 2005; 90(4): 2308 - 2313. [Abstract] [Full Text] [PDF]

				H. K. Palliser, J. J. Hirst, G. T. Ooi, G. E. Rice, N. L. Dellios, R. M. Escalona, H. C. Parkington, and I. R. Young Prostaglandin E and F Receptor Expression and Myometrial Sensitivity at Labor Onset in the Sheep Biol Reprod, April 1, 2005; 72(4): 937 - 943. [Abstract] [Full Text] [PDF]

				J. A. Arosh, S. K. Banu, S. Kimmins, P. Chapdelaine, L. A. MacLaren, and M. A. Fortier Effect of Interferon-{tau} on Prostaglandin Biosynthesis, Transport, and Signaling at the Time of Maternal Recognition of Pregnancy in Cattle: Evidence of Polycrine Actions of Prostaglandin E2 Endocrinology, November 1, 2004; 145(11): 5280 - 5293. [Abstract] [Full Text] [PDF]