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Expression and Regulation of Type 2 Iodothyronine Deiodinase in Rat Aorta Media

Department of Medicine II, Kansai Medical University, Moriguchi-City, Osaka 570, Japan

Address all correspondence and requests for reprints to: Nagaoki Toyoda, M.D., Department of Medicine II, Kansai Medical University, 10-15 Fumizono-Cho, Moriguchi-City, Osaka 570, Japan. E-mail: toyoda{at}takii.kmu.ac.jp.

Here, we have found that type 2 iodothyronine deiodinase (D2) is present in rat aorta media and that there is a circadian variation in the D2 expression. The D2 mRNA was approximately 4-fold higher at 0900 h than at 2100 h, and the activity was approximately 6-fold higher at noon than at 2100 h. The increase in aorta media D2 activity is preceded by the increase in its mRNA. The increase in D2 mRNA and activity in the circadian variation was reduced by the administration of prazosin, an ₁-adrenergic antagonist, and propranolol, a ß- adrenergic antagonist. Furthermore, phenylephrine, an ₁-adrenergic agonist, and isoproterenol, a ß-adrenergic agonist, caused a significant increase in D2 mRNA and activity. In the hypothyroid rats, aorta mediae D2 mRNA at both 0900 and 2100 h were not significantly different when compared with those in the euthyroid rats. On the other hand, aorta mediae D2 activity at both 1200 and 2100 h in the hypothyroid rats were approximately 2-fold higher. From these results, we suggest that D2 activity of rat aorta media is increased by both ₁- and ß-adrenergic stimulation, at least partly, at the pretranslational level. We also suggest that both ₁- and ß-adrenergic mechanisms may be involved, at least partly, in the circadian variation of the activity. In the hypothyroid state, the aorta media D2 activity is increased mainly by the posttranslational mechanism, and the similar circadian variation of the D2 expression is present as in the euthyroid state.

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