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Developmental Expression of Estrogen Receptor (ER) and ERß in the Hamster Ovary: Regulation by Follicle-Stimulating Hormone

Departments of Obstetrics and Gynecology (P.Y., J.W., S.K.R.), Pathology and Microbiology (Y.S.), and Cellular and Integrative Physiology (S.K.R.), University of Nebraska Medical Center, Omaha, Nebraska 68198-4515

Address all correspondence and requests for reprints to: Shyamal K. Roy, Ph.D., Durham Research Center 5013, Departments of Obstetrics and Gynecology and Physiology and Biophysics, University of Nebraska Medical Center, 984515 Nebraska Medical Center, Omaha, Nebraska 68198-4515. E-mail: skroy{at}unmc.edu.

Perinatal expression of estrogen receptor (ER) protein and mRNA and the influence of FSH on this process were examined by immunofluorescence and RT-PCR using ovaries from fetal (d 13–15 of gestation) and postnatal [postnatal d 1–15 (P1–P15)] hamsters and from 8-d-old hamsters exposed in utero to an anti-FSH serum on d 12 of gestation and saline or equine chorionic gonadotropin (eCG) on P1. A few somatic cells expressing ER immunoreactivity appeared first on d 14 of gestation and increased markedly by P8–P15 in the interstitial cells and granulosa cells of primordial follicles. In contrast, appreciable ERß immunoreactivity was localized on d 13 of gestation, and more cells expressed ERß immunoreactivity by P1–P8. By P7, ERß immunoreactivity was present in cells adjacent to the oocytes, and by P8, ERß was preferentially localized in the granulosa cells. Receptor immunoreactivities decreased markedly in P8 ovaries exposed in utero to the FSH antiserum but were reversed with postnatal eCG replacement. Oocytes and somatic cells expressed ER and ERß mRNA, and levels of ER mRNA in the ovary increased by P7–P8, corresponding to the appearance of primordial follicles. Thereafter, only ERß mRNA levels increased progressively with postnatal ovary development. Similar to ER protein, mRNA levels decreased significantly in FSH antiserum-treated ovaries but were restored by eCG. These results indicate that both ER subtypes are expressed in undifferentiated somatic cells and the oocytes during perinatal ovary development in the hamster; however, ERß expression segregates with the differentiation of granulosa cells. Furthermore, ER expression and differentiation of somatic cells to granulosa cells depend on perinatal FSH action.

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