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Estrogen Regulation of Neurokinin B Gene Expression in the Mouse Arcuate Nucleus Is Mediated by Estrogen Receptor

Wyeth Research, Collegeville, Pennsylvania 19426

Address all correspondence and requests for reprints to: Istvan Merchenthaler, Wyeth Research, 500 Arcola Road, Collegeville, Pennsylvania 19426. E-mail: merchei{at}wyeth.com.

Neurokinin B (NKB) gene expression is elevated in the infundibular (arcuate) nucleus of the hypothalamus in postmenopausal women. Estrogen replacement decreases both the number of NKB mRNA-expressing neurons and the level of expression within individual cells. Similarly, NKB gene expression is elevated in ovariectomized rats and reduced after estrogen treatment. The actions of estrogen in the brain can be mediated via either estrogen receptor (ER) or estrogen receptor ß (ERß). In the rodent arcuate nucleus (ARC), more ER- than ERß-containing cells are present, suggesting that ER might be directly responsible for estrogen regulation of NKB gene expression. However, an indirect effect via ERß could not be ruled out. Here we used ER knockout and ERß knockout mice to identify the type of ER responsible for mediating estrogen action on NKB gene expression in the ARC. Using in situ hybridization histochemistry, we have found that estrogen treatment significantly reduced NKB gene expression in the ARC of ovariectomized ERß knockout mice, but had no effect on NKB mRNA levels in ER knockout mice. These data indicate that ER mediates the increase in NKB gene expression associated with ovariectomy in rodents and might also be responsible for the increase in NKB in postmenopausal women.

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