This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hill, J. W. Articles by Levine, J. E. Search for Related Content PubMed PubMed Citation Articles by Hill, J. W. Articles by Levine, J. E.

Estrogen Induces Neuropeptide Y (NPY) Y1 Receptor Gene Expression and Responsiveness to NPY in Gonadotrope-Enriched Pituitary Cell Cultures

Department of Neurobiology and Physiology (J.W.H., J.H.U., M.X., J.E.L.), Northwestern University, Evanston, Illinois 60208; and Department of Physiology and Biophysics (J.H.U.), Finch University of Health Sciences/The Chicago Medical School, North Chicago, Illinois 60064

Address all correspondence and requests for reprints to: Jon E. Levine, Ph.D., Department of Neurobiology and Physiology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208. E-mail: jlevine{at}northwestern.edu.

We showed previously that neuropeptide Y1 receptor (Y1R) expression is increased in the hypothalamus on proestrus afternoon and that this up-regulation of Y1R mRNA may permit neuropeptide Y (NPY) to facilitate release of the preovulatory GnRH surge. Because NPY also modulates LH release directly, we examined steroid regulation of Y1R expression in the female rat anterior pituitary. Treatment of female rats with estrogen in vivo decreased the levels of Y1R mRNA in the whole pituitary gland. In lactotrope/somatotrope-enriched pituitary cells separated by unit gravity sedimentation, 17ß-estradiol (E₂) treatment likewise suppressed Y1R expression. In contrast, E₂ elevated Y1R mRNA in gonadotrope-enriched cell populations, indicating that estrogen regulates Y1R mRNA expression differently in gonadotropes vs. other pituitary cell types. After exposure to E₂, NPY augmented GnRH-induced LH release from gonadotrope-enriched cells in a manner requiring Y1R activation. Without steroid exposure, this augmentation disappeared, and with progesterone alone, NPY reduced GnRH-induced LH release. In addition, NPY inhibited prolactin secretion from primary pituitary cells in a steroid-free environment, but not in the presence of estrogen. These findings demonstrate that E₂ can directly up-regulate gonadotrope responsiveness to NPY and suggest that this action is mediated at least in part by E₂’s ability to stimulate Y1R gene expression in gonadotropes. Our observations are consistent with the idea that this regulatory mechanism represents a component of E₂’s positive feedback actions in pituitary gonadotropes. The biological importance of E₂’s opposite effects on Y1R expression in other pituitary cell types remains to be determined.

This article has been cited by other articles:

				F. Zammaretti, G. Panzica, and C. Eva Sex-dependent regulation of hypothalamic neuropeptide Y-Y1 receptor gene expression in moderate/high fat, high-energy diet-fed mice J. Physiol., September 1, 2007; 583(2): 445 - 454. [Abstract] [Full Text] [PDF]

				G. Segal-Lieberman, H. Rubinfeld, M. Glick, N. Kronfeld-Schor, and I. Shimon Melanin-concentrating hormone stimulates human growth hormone secretion: a novel effect of MCH on the hypothalamic-pituitary axis Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E982 - E988. [Abstract] [Full Text] [PDF]