help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology, doi:10.1210/en.2003-1676
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ábrahám, I. M.
Right arrow Articles by Herbison, A. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ábrahám, I. M.
Right arrow Articles by Herbison, A. E.
Endocrinology Vol. 145, No. 7 3055-3061
Copyright © 2004 by The Endocrine Society

Critical in Vivo Roles for Classical Estrogen Receptors in Rapid Estrogen Actions on Intracellular Signaling in Mouse Brain

István M. Ábrahám, Martin G. Todman, Kenneth S. Korach and Allan E. Herbison

Laboratory of Neuroendocrinology (I.M.A., M.G.T., A.E.H.), Babraham Institute, Cambridge CB2 4AT, United Kingdom; Neurobiology Research Group (I.M.A.), Hungarian Academy of Sciences, Eötvös Loránd University, H-1117 Budapest, Hungary; Receptor Biology Section (K.S.K.), Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709; and Centre for Neuroendocrinology and Department of Physiology (A.E.H.), University of Otago School of Medical Sciences, Dunedin, New Zealand

Address all correspondence and requests for reprints to: Allan E. Herbison, Centre for Neuroendocrinology, Department of Physiology, University of Otago School of Medical Sciences, P.O. Box 913, Dunedin, New Zealand. E-mail: allan.herbison{at}stonebow.otago.ac.nz.

Estrogen exerts classical genomic as well as rapid nongenomic actions on neurons. The mechanisms involved in rapid estrogen signaling are poorly defined, and the roles of the classical estrogen receptors (ERs {alpha} and ß) are unclear. We examined here the in vivo role of classical ERs in rapid estrogen actions by evaluating the estrogen-induced effects on two major signaling pathways within the brains of {alpha}ER-, ßER-, and double {alpha}ßER-knockout (ERKO) ovariectomized female mice. Estrogen significantly (P < 0.05) increased the numbers of phospho-cAMP response element binding protein (phospho-CREB)-immunoreactive cells in specific brain regions of wild-type mice in a time-dependent manner beginning within 15 min. In brain areas that express predominantly ERß, this response was absent in ßERKO mice, whereas brain regions that express mostly ER{alpha} displayed no change in {alpha}ERKO mice. In the medial preoptic nucleus (MPN), an area that expresses both ERs, the estrogen-induced phosphorylation of CREB was normal in both {alpha}ERKO and ßERKO mice. However, estrogen had no effect on CREB phosphorylation in the MPN, or any other brain region, in double {alpha}ßERKO animals. Estrogen was also found to increase MAPK phosphorylation levels in a rapid (<15 min) manner within the MPN. In contrast to CREB signaling, this effect was lost in either {alpha}ERKO or ßERKO mice. These data show that ER{alpha} and ERß play region- and pathway-specific roles in rapid estrogen actions throughout the brain. They further indicate an indispensable role for classical ERs in rapid estrogen actions in vivo and highlight the importance of ERs in coordinating both classical and rapid actions of estrogen.




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
Z. Chu, J. Andrade, M. A. Shupnik, and S. M. Moenter
Differential Regulation of Gonadotropin-Releasing Hormone Neuron Activity and Membrane Properties by Acutely Applied Estradiol: Dependence on Dose and Estrogen Receptor Subtype
J. Neurosci., April 29, 2009; 29(17): 5616 - 5627.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Zhao, C. Park, M. A. McDevitt, C. Glidewell-Kenney, P. Chambon, J. Weiss, J. L. Jameson, and J. E. Levine
p21-Activated kinase mediates rapid estradiol-negative feedback actions in the reproductive axis
PNAS, April 28, 2009; 106(17): 7221 - 7226.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. Dewing, A. Christensen, G. Bondar, and P. Micevych
Protein Kinase C Signaling in the Hypothalamic Arcuate Nucleus Regulates Sexual Receptivity in Female Rats
Endocrinology, December 1, 2008; 149(12): 5934 - 5942.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. Romano, K. Lee, I. M. Abraham, C. L. Jasoni, and A. E. Herbison
Nonclassical Estrogen Modulation of Presynaptic GABA Terminals Modulates Calcium Dynamics in Gonadotropin-Releasing Hormone Neurons
Endocrinology, November 1, 2008; 149(11): 5335 - 5344.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
G. Rasier, A.-S. Parent, A. Gerard, R. Denooz, M.-C. Lebrethon, C. Charlier, and J.-P. Bourguignon
Mechanisms of Interaction of Endocrine-Disrupting Chemicals with Glutamate-Evoked Secretion of Gonadotropin-Releasing Hormone
Toxicol. Sci., March 1, 2008; 102(1): 33 - 41.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. M. McCARTHY
Estradiol and the Developing Brain
Physiol Rev, January 1, 2008; 88(1): 91 - 134.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. S. Lalmansingh and R. M. Uht
Estradiol Regulates Corticotropin-Releasing Hormone Gene (crh) Expression in a Rapid and Phasic Manner that Parallels Estrogen Receptor-{alpha} and - Recruitment to a 3',5'-Cyclic Adenosine 5'-Monophosphate Regulatory Region of the Proximal crh Promoter
Endocrinology, January 1, 2008; 149(1): 346 - 357.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
S. R. Hammes and E. R. Levin
Extranuclear Steroid Receptors: Nature and Actions
Endocr. Rev., December 1, 2007; 28(7): 726 - 741.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. I. Boulware, H. Kordasiewicz, and P. G. Mermelstein
Caveolin Proteins Are Essential for Distinct Effects of Membrane Estrogen Receptors in Neurons
J. Neurosci., September 12, 2007; 27(37): 9941 - 9950.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. Dewing, M. I. Boulware, K. Sinchak, A. Christensen, P. G. Mermelstein, and P. Micevych
Membrane Estrogen Receptor-{alpha} Interactions with Metabotropic Glutamate Receptor 1a Modulate Female Sexual Receptivity in Rats
J. Neurosci., August 29, 2007; 27(35): 9294 - 9300.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. C. Trainor, S. Lin, M. S. Finy*, M. R. Rowland, and R. J. Nelson
Photoperiod reverses the effects of estrogens on male aggression via genomic and nongenomic pathways
PNAS, June 5, 2007; 104(23): 9840 - 9845.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
N. Vasudevan and D. W. Pfaff
Membrane-Initiated Actions of Estrogens in Neuroendocrinology: Emerging Principles
Endocr. Rev., February 1, 2007; 28(1): 1 - 19.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. Pedram, M. Razandi, and E. R. Levin
Nature of Functional Estrogen Receptors at the Plasma Membrane
Mol. Endocrinol., September 1, 2006; 20(9): 1996 - 2009.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. M. Szego, K. Barabas, J. Balog, N. Szilagyi, K. S. Korach, G. Juhasz, and I. M. Abraham
Estrogen Induces Estrogen Receptor {alpha}-Dependent cAMP Response Element-Binding Protein Phosphorylation via Mitogen Activated Protein Kinase Pathway in Basal Forebrain Cholinergic Neurons In Vivo
J. Neurosci., April 12, 2006; 26(15): 4104 - 4110.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
M. D'Astous, P. Mendez, M. Morissette, L. M. Garcia-Segura, and T. Di Paolo
Implication of the Phosphatidylinositol-3 Kinase/Protein Kinase B Signaling Pathway in the Neuroprotective Effect of Estradiol in the Striatum of 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Mice
Mol. Pharmacol., April 1, 2006; 69(4): 1492 - 1498.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Zsarnovszky, H. H. Le, H.-S. Wang, and S. M. Belcher
Ontogeny of Rapid Estrogen-Mediated Extracellular Signal-Regulated Kinase Signaling in the Rat Cerebellar Cortex: Potent Nongenomic Agonist and Endocrine Disrupting Activity of the Xenoestrogen Bisphenol A
Endocrinology, December 1, 2005; 146(12): 5388 - 5396.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
Y. Merot, F. Ferriere, E. Debroas, G. Flouriot, D. Duval, and C. Saligaut
Estrogen receptor alpha mediates neuronal differentiation and neuroprotection in PC12 cells: critical role of the A/B domain of the receptor
J. Mol. Endocrinol., October 1, 2005; 35(2): 257 - 267.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
E. R. Levin
Integration of the Extranuclear and Nuclear Actions of Estrogen
Mol. Endocrinol., August 1, 2005; 19(8): 1951 - 1959.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. A. Arreguin-Arevalo and T. M. Nett
A Nongenomic Action of 17{beta}-Estradiol as the Mechanism Underlying the Acute Suppression of Secretion of Luteinizing Hormone
Biol Reprod, July 1, 2005; 73(1): 115 - 122.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. I. Boulware, J. P. Weick, B. R. Becklund, S. P. Kuo, R. D. Groth, and P. G. Mermelstein
Estradiol Activates Group I and II Metabotropic Glutamate Receptor Signaling, Leading to Opposing Influences on cAMP Response Element-Binding Protein
J. Neurosci., May 18, 2005; 25(20): 5066 - 5078.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2004 by The Endocrine Society