help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology, doi:10.1210/en.2004-0836
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
146/1/156    most recent
Author Manuscript (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Navarro, V. M.
Right arrow Articles by Tena-Sempere, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Navarro, V. M.
Right arrow Articles by Tena-Sempere, M.
Endocrinology Vol. 146, No. 1 156-163
Copyright © 2005 by The Endocrine Society

Characterization of the Potent Luteinizing Hormone-Releasing Activity of KiSS-1 Peptide, the Natural Ligand of GPR54

V. M. Navarro1, J. M. Castellano1, R. Fernández-Fernández, S. Tovar, J. Roa, A. Mayen, R. Nogueiras, M. J. Vazquez, M. L. Barreiro, P. Magni, E. Aguilar, C. Dieguez, L. Pinilla and M. Tena-Sempere

Department of Cell Biology, Physiology and Immunology (V.M.N., J.M.C., R.F.-F., J.R., A.M., M.L.B., E.A., L.P., M.T.-S.), University of Córdoba, 14004 Córdoba, Spain; Institute of Endocrinology (P.M.), University of Milan, 20133 Milan, Italy; and Department of Physiology (S.T., R.N., M.J.V., C.D.), University of Santiago de Compostela, 15705 Santiago de Compostela, Spain

Address all correspondence and requests for reprints to: Manuel Tena-Sempere, Physiology Section, Department of Cell Biology, Physiology, and Immunology, Faculty of Medicine, University of Córdoba, Avda. Menéndez Pidal s/n, 14004 Córdoba, Spain. E-mail: fi1tesem{at}uco.es.

Loss-of-function mutations of the gene encoding GPR54, the putative receptor for the KiSS-1-derived peptide metastin, have been recently associated with hypogonadotropic hypogonadism, in both rodents and humans. Yet the actual role of the KiSS-1/GPR54 system in the neuroendocrine control of gonadotropin secretion remains largely unexplored. To initiate such analysis, the effects of KiSS-1 peptide on LH secretion were monitored using in vivo and in vitro settings under different experimental conditions. Central intracerebroventricular administration of KiSS-1 peptide potently elicited LH secretion in vivo over a range of doses from 10 pmol to 1 nmol. The effect of centrally injected KiSS-1 appeared to be mediated via the hypothalamic LHRH. However, no effect of central administration of KiSS-1 was detected on relative LHRH mRNA levels. Likewise, systemic (ip and iv) injection of KiSS-1 markedly stimulated LH secretion. This effect was similar in terms of maximum response to that of central administration of KiSS-1 and might be partially attributed to its ability to stimulate LH secretion directly at the pituitary. Finally, the LH-releasing activity of KiSS-1 was persistently observed after blockade of endogenous excitatory amino acid and nitric oxide pathways, i.e. relevant neurotransmitters in the neuroendocrine control of LH secretion. In summary, our results provide solid evidence for a potent stimulatory effect of KiSS-1 on LH release, acting at central levels (likely the hypothalamus) and eventually at the pituitary, and further document a novel role of the KiSS-1/GPR54 system as a relevant downstream element in the neuroendocrine network governing LH secretion.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
X. d'Anglemont de Tassigny, L. A. Fagg, M. B. L. Carlton, and W. H. Colledge
Kisspeptin Can Stimulate Gonadotropin-Releasing Hormone (GnRH) Release by a Direct Action at GnRH Nerve Terminals
Endocrinology, August 1, 2008; 149(8): 3926 - 3932.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. L. Keen, F. H. Wegner, S. R. Bloom, M. A. Ghatei, and E. Terasawa
An Increase in Kisspeptin-54 Release Occurs with the Pubertal Increase in Luteinizing Hormone-Releasing Hormone-1 Release in the Stalk-Median Eminence of Female Rhesus Monkeys in Vivo
Endocrinology, August 1, 2008; 149(8): 4151 - 4157.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
C. A Lents, N. L Heidorn, C R. Barb, and J J. Ford
Central and peripheral administration of kisspeptin activates gonadotropin but not somatotropin secretion in prepubertal gilts
Reproduction, June 1, 2008; 135(6): 879 - 887.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. Roa, E. Vigo, D. Garcia-Galiano, J. M. Castellano, V. M. Navarro, R. Pineda, C. Dieguez, E. Aguilar, L. Pinilla, and M. Tena-Sempere
Desensitization of gonadotropin responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states
Am J Physiol Endocrinol Metab, June 1, 2008; 294(6): E1088 - E1096.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Kanda, Y. Akazome, T. Matsunaga, N. Yamamoto, S. Yamada, H. Tsukamura, K.-i. Maeda, and Y. Oka
Identification of KiSS-1 Product Kisspeptin and Steroid-Sensitive Sexually Dimorphic Kisspeptin Neurons in Medaka (Oryzias latipes)
Endocrinology, May 1, 2008; 149(5): 2467 - 2476.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. T. Smith, A. Rao, A. Pereira, A. Caraty, R. P. Millar, and I. J. Clarke
Kisspeptin Is Present in Ovine Hypophysial Portal Blood But Does Not Increase during the Preovulatory Luteinizing Hormone Surge: Evidence that Gonadotropes Are Not Direct Targets of Kisspeptin in Vivo
Endocrinology, April 1, 2008; 149(4): 1951 - 1959.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Quaynor, L. Hu, P. K. Leung, H. Feng, N. Mores, L. Z. Krsmanovic, and K. J. Catt
Expression of a Functional G Protein-Coupled Receptor 54-Kisspeptin Autoregulatory System in Hypothalamic Gonadotropin-Releasing Hormone Neurons
Mol. Endocrinol., December 1, 2007; 21(12): 3062 - 3070.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. L. Goodman, M. N. Lehman, J. T. Smith, L. M. Coolen, C. V. R. de Oliveira, M. R. Jafarzadehshirazi, A. Pereira, J. Iqbal, A. Caraty, P. Ciofi, et al.
Kisspeptin Neurons in the Arcuate Nucleus of the Ewe Express Both Dynorphin A and Neurokinin B
Endocrinology, December 1, 2007; 148(12): 5752 - 5760.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Caraty, J. T. Smith, D. Lomet, S. Ben Said, A. Morrissey, J. Cognie, B. Doughton, G. Baril, C. Briant, and I. J. Clarke
Kisspeptin Synchronizes Preovulatory Surges in Cyclical Ewes and Causes Ovulation in Seasonally Acyclic Ewes
Endocrinology, November 1, 2007; 148(11): 5258 - 5267.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. M. Dungan, M. L. Gottsch, H. Zeng, A. Gragerov, J. E. Bergmann, D. K. Vassilatis, D. K. Clifton, and R. A. Steiner
The Role of Kisspeptin GPR54 Signaling in the Tonic Regulation and Surge Release of Gonadotropin-Releasing Hormone/Luteinizing Hormone
J. Neurosci., October 31, 2007; 27(44): 12088 - 12095.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
W. S. Dhillo, O. B. Chaudhri, E. L. Thompson, K. G. Murphy, M. Patterson, R. Ramachandran, G. K. Nijher, V. Amber, A. Kokkinos, M. Donaldson, et al.
Kisspeptin-54 Stimulates Gonadotropin Release Most Potently during the Preovulatory Phase of the Menstrual Cycle in Women
J. Clin. Endocrinol. Metab., October 1, 2007; 92(10): 3958 - 3966.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. Li, D. Mitchell, J. Luo, Z. Yi, S.-G. Cho, J. Guo, X. Li, G. Ning, X. Wu, and M. Liu
Estrogen Regulates KiSS1 Gene Expression through Estrogen Receptor {alpha} and SP Protein Complexes
Endocrinology, October 1, 2007; 148(10): 4821 - 4828.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
D. Marot, I. Bieche, C. Aumas, S. Esselin, C. Bouquet, S. Vacher, G. Lazennec, M. Perricaudet, F. Kuttenn, R. Lidereau, et al.
High tumoral levels of Kiss1 and G-protein-coupled receptor 54 expression are correlated with poor prognosis of estrogen receptor-positive breast tumors
Endocr. Relat. Cancer, September 1, 2007; 14(3): 691 - 702.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
B. J Todd, G. S Fraley, A. C Peck, G. J Schwartz, and A. M Etgen
Central Insulin-Like Growth Factor 1 Receptors Play Distinct Roles in the Control of Reproduction, Food Intake, and Body Weight in Female Rats
Biol Reprod, September 1, 2007; 77(3): 492 - 503.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. S. Kauffman, M. L. Gottsch, J. Roa, A. C. Byquist, A. Crown, D. K. Clifton, G. E. Hoffman, R. A. Steiner, and M. Tena-Sempere
Sexual Differentiation of Kiss1 Gene Expression in the Brain of the Rat
Endocrinology, April 1, 2007; 148(4): 1774 - 1783.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
Y. Tenenbaum-Rakover, M. Commenges-Ducos, A. Iovane, C. Aumas, O. Admoni, and N. de Roux
Neuroendocrine Phenotype Analysis in Five Patients with Isolated Hypogonadotropic Hypogonadism due to a L102P Inactivating Mutation of GPR54
J. Clin. Endocrinol. Metab., March 1, 2007; 92(3): 1137 - 1144.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. J. Greives, A. O. Mason, M.-A. L. Scotti, J. Levine, E. D. Ketterson, L. J. Kriegsfeld, and G. E. Demas
Environmental Control of Kisspeptin: Implications for Seasonal Reproduction
Endocrinology, March 1, 2007; 148(3): 1158 - 1166.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
E. J. Mead, J. J. Maguire, R. E. Kuc, and A. P. Davenport
Kisspeptins Are Novel Potent Vasoconstrictors in Humans, with a Discrete Localization of Their Receptor, G Protein-Coupled Receptor 54, to Atherosclerosis-Prone Vessels
Endocrinology, January 1, 2007; 148(1): 140 - 147.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. M. Castellano, V. M. Navarro, R. Fernandez-Fernandez, J. Roa, E. Vigo, R. Pineda, R. A. Steiner, E. Aguilar, L. Pinilla, and M. Tena-Sempere
Effects of galanin-like peptide on luteinizing hormone secretion in the rat: sexually dimorphic responses and enhanced sensitivity at male puberty
Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1281 - E1289.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
W. S. Dhillo, P. Savage, K. G. Murphy, O. B. Chaudhri, M. Patterson, G. M. Nijher, V. M. Foggo, G. S. Dancey, H. Mitchell, M. J. Seckl, et al.
Plasma kisspeptin is raised in patients with gestational trophoblastic neoplasia and falls during treatment
Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E878 - E884.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. L. Thompson, K. G. Murphy, M. Patterson, G. A. Bewick, G. W. H. Stamp, A. E. Curtis, J. H. Cooke, P. H. Jethwa, J. F. Todd, M. A. Ghatei, et al.
Chronic subcutaneous administration of kisspeptin-54 causes testicular degeneration in adult male rats
Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E1074 - E1082.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J. M. Castellano, V. M. Navarro, R. Fernandez-Fernandez, J. Roa, E. Vigo, R. Pineda, C. Dieguez, E. Aguilar, L. Pinilla, and M. Tena-Sempere
Expression of Hypothalamic KiSS-1 System and Rescue of Defective Gonadotropic Responses by Kisspeptin in Streptozotocin-Induced Diabetic Male Rats
Diabetes, September 1, 2006; 55(9): 2602 - 2610.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
M. Tena-Sempere
GPR54 and kisspeptin in reproduction
Hum. Reprod. Update, September 1, 2006; 12(5): 631 - 639.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Tovar, M. J. Vazquez, V. M. Navarro, R. Fernandez-Fernandez, J. M. Castellano, E. Vigo, J. Roa, F. F. Casanueva, E. Aguilar, L. Pinilla, et al.
Effects of Single or Repeated Intravenous Administration of Kisspeptin upon Dynamic LH Secretion in Conscious Male Rats
Endocrinology, June 1, 2006; 147(6): 2696 - 2704.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Roa, E. Vigo, J. M. Castellano, V. M. Navarro, R. Fernandez-Fernandez, F. F. Casanueva, C. Dieguez, E. Aguilar, L. Pinilla, and M. Tena-Sempere
Hypothalamic Expression of KiSS-1 System and Gonadotropin-Releasing Effects of Kisspeptin in Different Reproductive States of the Female Rat
Endocrinology, June 1, 2006; 147(6): 2864 - 2878.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
V. M. Navarro, R. Fernandez-Fernandez, R. Nogueiras, E. Vigo, S. Tovar, N. Chartrel, O. Le Marec, J. Leprince, E. Aguilar, L. Pinilla, et al.
Novel role of 26RFa, a hypothalamic RFamide orexigenic peptide, as putative regulator of the gonadotropic axis
J. Physiol., May 15, 2006; 573(1): 237 - 249.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. C. Martini, R. Fernandez-Fernandez, S. Tovar, V. M. Navarro, E. Vigo, M. J. Vazquez, J. S. Davies, N. M. Thompson, E. Aguilar, L. Pinilla, et al.
Comparative Analysis of the Effects of Ghrelin and Unacylated Ghrelin on Luteinizing Hormone Secretion in Male Rats
Endocrinology, May 1, 2006; 147(5): 2374 - 2382.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. B. Seminara, M. J. DiPietro, S. Ramaswamy, W. F. Crowley Jr., and T. M. Plant
Continuous Human Metastin 45-54 Infusion Desensitizes G Protein-Coupled Receptor 54-Induced Gonadotropin-Releasing Hormone Release Monitored Indirectly in the Juvenile Male Rhesus Monkey (Macaca mulatta): A Finding with Therapeutic Implications
Endocrinology, May 1, 2006; 147(5): 2122 - 2126.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
J. T Smith, D. K Clifton, and R. A Steiner
Regulation of the neuroendocrine reproductive axis by kisspeptin-GPR54 signaling.
Reproduction, April 1, 2006; 131(4): 623 - 630.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
J. D. Veldhuis, J. N. Roemmich, E. J. Richmond, and C. Y. Bowers
Somatotropic and Gonadotropic Axes Linkages in Infancy, Childhood, and the Puberty-Adult Transition
Endocr. Rev., April 1, 2006; 27(2): 101 - 140.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. R. Ojeda, A. Lomniczi, C. Mastronardi, S. Heger, C. Roth, A.-S. Parent, V. Matagne, and A. E. Mungenast
Minireview: The Neuroendocrine Regulation of Puberty: Is the Time Ripe for a Systems Biology Approach?
Endocrinology, March 1, 2006; 147(3): 1166 - 1174.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. M. Dungan, D. K Clifton, and R. A. Steiner
Minireview: Kisspeptin Neurons as Central Processors in the Regulation of Gonadotropin-Releasing Hormone Secretion
Endocrinology, March 1, 2006; 147(3): 1154 - 1158.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Pompolo, A. Pereira, K. M. Estrada, and I. J. Clarke
Colocalization of Kisspeptin and Gonadotropin-Releasing Hormone in the Ovine Brain
Endocrinology, February 1, 2006; 147(2): 804 - 810.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. M. Plant, S. Ramaswamy, and M. J. DiPietro
Repetitive Activation of Hypothalamic G Protein-Coupled Receptor 54 with Intravenous Pulses of Kisspeptin in the Juvenile Monkey (Macaca mulatta) Elicits a Sustained Train of Gonadotropin-Releasing Hormone Discharges
Endocrinology, February 1, 2006; 147(2): 1007 - 1013.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S.-K. Han, M. L. Gottsch, K. J. Lee, S. M. Popa, J. T. Smith, S. K. Jakawich, D. K. Clifton, R. A. Steiner, and A. E. Herbison
Activation of Gonadotropin-Releasing Hormone Neurons by Kisspeptin as a Neuroendocrine Switch for the Onset of Puberty
J. Neurosci., December 7, 2005; 25(49): 11349 - 11356.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
W. S. Dhillo, O. B. Chaudhri, M. Patterson, E. L. Thompson, K. G. Murphy, M. K. Badman, B. M. McGowan, V. Amber, S. Patel, M. A. Ghatei, et al.
Kisspeptin-54 Stimulates the Hypothalamic-Pituitary Gonadal Axis in Human Males
J. Clin. Endocrinol. Metab., December 1, 2005; 90(12): 6609 - 6615.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. C. Arai, Y.-F. Xia, E. Suzuki, M. Kessler, O. Civelli, and H.-P. Nothacker
Cancer Metastasis-Suppressing Peptide Metastin Upregulates Excitatory Synaptic Transmission in Hippocampal Dentate Granule Cells
J Neurophysiol, November 1, 2005; 94(5): 3648 - 3652.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Tena-Sempere
Hypothalamic KiSS-1: The Missing Link in Gonadotropin Feedback Control?
Endocrinology, September 1, 2005; 146(9): 3683 - 3685.
[Full Text] [PDF]

Home page
 EndocrinologyHome page
J. T. Smith, M. J. Cunningham, E. F. Rissman, D. K Clifton, and R. A. Steiner
Regulation of Kiss1 Gene Expression in the Brain of the Female Mouse
Endocrinology, September 1, 2005; 146(9): 3686 - 3692.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. M. Castellano, V. M. Navarro, R. Fernandez-Fernandez, R. Nogueiras, S. Tovar, J. Roa, M. J. Vazquez, E. Vigo, F. F. Casanueva, E. Aguilar, et al.
Changes in Hypothalamic KiSS-1 System and Restoration of Pubertal Activation of the Reproductive Axis by Kisspeptin in Undernutrition
Endocrinology, September 1, 2005; 146(9): 3917 - 3925.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. T. Smith, H. M. Dungan, E. A. Stoll, M. L. Gottsch, R. E. Braun, S. M. Eacker, D. K Clifton, and R. A. Steiner
Differential Regulation of KiSS-1 mRNA Expression by Sex Steroids in the Brain of the Male Mouse
Endocrinology, July 1, 2005; 146(7): 2976 - 2984.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. B. Seminara and U. B. Kaiser
New Gatekeepers of Reproduction: GPR54 and Its Cognate Ligand, KiSS-1
Endocrinology, April 1, 2005; 146(4): 1686 - 1688.
[Full Text] [PDF]

Home page
 EndocrinologyHome page
V. M. Navarro, J. M. Castellano, R. Fernandez-Fernandez, S. Tovar, J. Roa, A. Mayen, M. L. Barreiro, F. F. Casanueva, E. Aguilar, C. Dieguez, et al.
Effects of KiSS-1 Peptide, the Natural Ligand of GPR54, on Follicle-Stimulating Hormone Secretion in the Rat
Endocrinology, April 1, 2005; 146(4): 1689 - 1697.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Shahab, C. Mastronardi, S. B. Seminara, W. F. Crowley, S. R. Ojeda, and T. M. Plant
Increased hypothalamic GPR54 signaling: A potential mechanism for initiation of puberty in primates
PNAS, February 8, 2005; 102(6): 2129 - 2134.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2005 by The Endocrine Society