This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lewis, G. F. Articles by Adeli, K. Search for Related Content PubMed PubMed Citation Articles by Lewis, G. F. Articles by Adeli, K.

Intestinal Lipoprotein Overproduction, a Newly Recognized Component of Insulin Resistance, Is Ameliorated by the Insulin Sensitizer Rosiglitazone: Studies in the Fructose-Fed Syrian Golden Hamster

Departments of Medicine and Physiology (G.F.L., K.U., L.S.), Division of Endocrinology and Metabolism, and the Department of Laboratory Medicine and Pathobiology (M.N., M.H., K.A.), Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada M5G 2C4

Address all correspondence and requests for reprints to: Gary F. Lewis, Toronto General Hospital, 200 Elizabeth Street, EN11-229, Toronto, Ontario, Canada M5G 2C4. E-mail: gary.lewis{at}uhn.on.ca.

We investigated whether intestinal lipoprotein overproduction in a fructose-fed, insulin-resistant hamster model is prevented with insulin sensitization. Syrian Golden hamsters were fed either chow, 60% fructose for 5 wk, chow for 5 wk with the insulin sensitizer rosiglitazone added for the last 3 wk, or 60% fructose plus rosiglitazone. In vivo Triton studies showed a 2- to 3-fold increase in the large (Svedberg unit > 400) and smaller (S_f 100–400) triglyceride-rich lipoprotein particle apolipoprotein B48 (apoB48) but not triglyceride secretion with fructose feeding in the fasted state (P < 0.01) and partial normalization with rosiglitazone in fructose-fed hamsters. Ex vivo pulse-chase labeling of enterocytes confirmed the oversecretion of apoB48 lipoproteins with fructose feeding. Intestinal lipoprotein oversecretion was associated with increased expression of microsomal triglyceride transfer protein expression. With rosiglitazone treatment of fructose-fed hamsters, there was approximately 50% reduction in apoB48 secretion from primary cultured enterocytes and amelioration of the elevated microsomal triglyceride transfer protein mass and activity in fructose-fed hamsters. In contrast, in the postprandial state, the major differences between nutritional and drug intervention protocols were evident in triglyceride-rich lipoprotein triglyceride and not apoB48 secretion rates. The data suggest that intestinal lipoprotein overproduction can be ameliorated with the insulin sensitizer rosiglitazone.

This article has been cited by other articles:

				J. L. Sievenpiper, A. J. Carleton, S. Chatha, H. Y. Jiang, R. J. de Souza, J. Beyene, C. W.C. Kendall, and D. J.A. Jenkins Heterogeneous Effects of Fructose on Blood Lipids in Individuals With Type 2 Diabetes: Systematic review and meta-analysis of experimental trials in humans Diabetes Care, October 1, 2009; 32(10): 1930 - 1937. [Abstract] [Full Text] [PDF]

				S. J. Hernandez Vallejo, M. Alqub, S. Luquet, C. Cruciani-Guglielmacci, P. Delerive, J.-M. Lobaccaro, A.-D. Kalopissis, J. Chambaz, M. Rousset, and J.-M. Lacorte Short-term adaptation of postprandial lipoprotein secretion and intestinal gene expression to a high-fat diet Am J Physiol Gastrointest Liver Physiol, April 1, 2009; 296(4): G782 - G792. [Abstract] [Full Text] [PDF]

				M. Pavlic, R. Valero, H. Duez, C. Xiao, L. Szeto, B. W. Patterson, and G. F. Lewis Triglyceride-Rich Lipoprotein-Associated Apolipoprotein C-III Production Is Stimulated by Plasma Free Fatty Acids in Humans Arterioscler Thromb Vasc Biol, September 1, 2008; 28(9): 1660 - 1665. [Abstract] [Full Text] [PDF]

				H. Duez, B. Lamarche, R. Valero, M. Pavlic, S. Proctor, C. Xiao, L. Szeto, B. W. Patterson, and G. F. Lewis Both Intestinal and Hepatic Lipoprotein Production Are Stimulated by an Acute Elevation of Plasma Free Fatty Acids in Humans Circulation, May 6, 2008; 117(18): 2369 - 2376. [Abstract] [Full Text] [PDF]

				H. Duez, B. Lamarche, K. D. Uffelman, R. Valero, L. Szeto, S. Lemieux, J. S. Cohn, and G. F. Lewis Dissociation between the Insulin-Sensitizing Effect of Rosiglitazone and Its Effect on Hepatic and Intestinal Lipoprotein Production J. Clin. Endocrinol. Metab., May 1, 2008; 93(5): 1722 - 1729. [Abstract] [Full Text] [PDF]

				C.-M. Lo, B. K. Nordskog, A. M. Nauli, S. Zheng, S. B. vonLehmden, Q. Yang, D. Lee, L. L. Swift, N. O. Davidson, and P. Tso Why does the gut choose apolipoprotein B48 but not B100 for chylomicron formation? Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G344 - G352. [Abstract] [Full Text] [PDF]

				J.-C. Hogue, B. Lamarche, A. J. Tremblay, J. Bergeron, C. Gagne, and P. Couture Evidence of increased secretion of apolipoprotein B-48-containing lipoproteins in subjects with type 2 diabetes J. Lipid Res., June 1, 2007; 48(6): 1336 - 1342. [Abstract] [Full Text] [PDF]

				M. Laplante, W. T. Festuccia, G. Soucy, Y. Gelinas, J. Lalonde, and Y. Deshaies Involvement of adipose tissues in the early hypolipidemic action of PPAR{gamma} agonism in the rat Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2007; 292(4): R1408 - R1417. [Abstract] [Full Text] [PDF]

				S. Qu, D. Su, J. Altomonte, A. Kamagate, J. He, G. Perdomo, T. Tse, Y. Jiang, and H. H. Dong PPAR{alpha} mediates the hypolipidemic action of fibrates by antagonizing FoxO1 Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E421 - E434. [Abstract] [Full Text] [PDF]

				H. Duez, B. Lamarche, K. D. Uffelman, R. Valero, J. S. Cohn, and G. F. Lewis Hyperinsulinemia Is Associated With Increased Production Rate of Intestinal Apolipoprotein B-48-Containing Lipoproteins in Humans Arterioscler Thromb Vasc Biol, June 1, 2006; 26(6): 1357 - 1363. [Abstract] [Full Text] [PDF]