This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mueller, N. K. Articles by Herman, J. P. Search for Related Content PubMed PubMed Citation Articles by Mueller, N. K. Articles by Herman, J. P. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB SODIUM CHLORIDE VASOPRESSIN

Activity-Dependent Modulation of Neurotransmitter Innervation to Vasopressin Neurons of the Supraoptic Nucleus

Department of Psychiatry (N.K.M., J.P.H.) and Cell Biology, Neurobiology, and Anatomy (J.P.H.), University of Cincinnati Medical Center, Cincinnati, Ohio 45237-0506; Department of Cell and Molecular Biology (S.D.), Tulane University, New Orleans, Louisiana 70118; and Department of Cell Biology and Neuroscience (C.M.P.), Montana State University, Bozeman, Montana 59717-3148

Address all correspondence and requests for reprints to: James P. Herman, Ph.D., Department of Psychiatry, University of Cincinnati, 2170 East Galbraith Road, Cincinnati, Ohio 45237-0506. E-mail james.herman{at}uc.edu.

Confocal microscopy was used to assess activity-dependent neuroplasticity in neurotransmitter innervation of vasopressin immunoreactive magnocellular neurons in the supraoptic nucleus (SON). Vesicular glutamate transporter 2, glutamic acid decarboxylase, and dopamine ß-hydroxylase (DBH) synaptic boutons were visualized in apposition to vasopressin neurons in the SON. A decrease in DBH synaptic boutons per cell was seen upon salt loading, indicating diminished noradrenergic/adrenergic innervation. Loss of DBH appositions to vasopressin neurons was associated with a general loss of DBH immunoreactivity in the SON. In contrast, the number of vesicular glutamate transporter 2 synaptic boutons per neuron increased with salt loading, consistent with increased glutamatergic drive of magnocellular SON neurons. Salt loading also caused an increase in the total number of glutamic acid decarboxylase synaptic boutons on vasopressinergic neurons, suggesting enhanced inhibitory innervation as well. These studies indicate that synaptic plasticity compensates for increased secretory demand and may indeed underlie increased secretion, perhaps via neurotransmitter-specific, activity-related changes in synaptic contacts on vasopressinergic magnocellular neurons in the SON.

This article has been cited by other articles:

				R. Zhang, B. A. Packard, M. Tauchi, D. A. D'Alessio, and J. P. Herman Glucocorticoid regulation of preproglucagon transcription and RNA stability during stress PNAS, April 7, 2009; 106(14): 5913 - 5918. [Abstract] [Full Text] [PDF]

				T. P. Vahl, M. Tauchi, T. S. Durler, E. E. Elfers, T. M. Fernandes, R. D. Bitner, K. S. Ellis, S. C. Woods, R. J. Seeley, J. P. Herman, et al. Glucagon-Like Peptide-1 (GLP-1) Receptors Expressed on Nerve Terminals in the Portal Vein Mediate the Effects of Endogenous GLP-1 on Glucose Tolerance in Rats Endocrinology, October 1, 2007; 148(10): 4965 - 4973. [Abstract] [Full Text] [PDF]

				K. T. Higa-Taniguchi, F. C. P. Silva, H. M. V. Silva, L. C. Michelini, and J. E. Stern Exercise training-induced remodeling of paraventricular nucleus (nor)adrenergic innervation in normotensive and hypertensive rats Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2007; 292(4): R1717 - R1727. [Abstract] [Full Text] [PDF]

				S. Di, C. Boudaba, I. R. Popescu, F.-J. Weng, C. Harris, V. L. Marcheselli, N. G. Bazan, and J. G. Tasker Activity-dependent release and actions of endocannabinoids in the rat hypothalamic supraoptic nucleus J. Physiol., December 15, 2005; 569(3): 751 - 760. [Abstract] [Full Text] [PDF]