help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology, doi:10.1210/en.2005-0759
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
146/12/5522    most recent
Author Manuscript (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cai, Z.
Right arrow Articles by Stocco, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cai, Z.
Right arrow Articles by Stocco, C.
Endocrinology Vol. 146, No. 12 5522-5532
Copyright © 2005 by The Endocrine Society

Expression and Regulation of Progestin Membrane Receptors in the Rat Corpus Luteum

Zailong Cai and Carlos Stocco

Department of Obstetrics, Gynecology, and Reproductive Science, Yale School of Medicine, New Haven, Connecticut 06520

Address all correspondence and requests for reprints to: Carlos Stocco, Department of Obstetrics, Gynecology, and Reproductive Science, 333 Cedar Street, New Haven, Connecticut 06520. E-mail: carlos.stocco{at}yale.edu.

Despite evidence strongly supporting progesterone’s autocrine actions in the rat corpus luteum (CL), classical progesterone receptors (PR) have not been detected in this gland. Alternatively, in several other systems, progestins have been reported to activate nongenomic pathways via putative progestin membrane receptors (PMRs). The aim of this investigation was to determine whether rat CL membranes bind progestins and contain PMR homologs and whether these proteins are expressed during CL development in a manner that parallels luteal function. We found that luteal cell membranes specifically bind progesterone. Low levels of progesterone and 20{alpha}-dihydroprogesterone decreased binding of [3H]progesterone, whereas androstenedione, 17{alpha}-hydroxyprogesterone, and pregnenolone were less potent. Other steroids, including corticosterone, mifepristone, and estradiol, were ineffective. We found that the rat CL expresses five genes previously postulated to encode for putative PMRs: PMR{alpha}, PMRß, PMR{gamma}, PR membrane component 1 (PRMC1), and Rda288. Pmr{alpha}, Pmr{gamma}, and Prmc1 transcripts rose steadily during pregnancy whereas Pmrß and Rda288 remained constant. Just before parturition, concomitant with falling progesterone levels, Pmr{alpha}, Pmrß, and Prmc1 decreased. Luteal PMR{alpha} and PRMC1 protein levels were lower in samples taken at the end of pregnancy compared with midpregnancy samples. Ergocriptine, which inhibits the secretion of prolactin, the primary luteotrophic hormone in the rat CL, reduced Pmr{alpha}, Pmrß, and Prmc1 expression significantly. Ergocriptine effects were prevented by coadministration of prolactin. These findings provide evidence for the expression and regulation of putative membrane-bound progestin-binding proteins in the rat CL, a tissue that does not express detectable levels of nuclear progesterone receptors.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Glaser, S. DeMorrow, H. Francis, Y. Ueno, E. Gaudio, S. Vaculin, J. Venter, A. Franchitto, P. Onori, B. Vaculin, et al.
Progesterone stimulates the proliferation of female and male cholangiocytes via autocrine/paracrine mechanisms
Am J Physiol Gastrointest Liver Physiol, July 1, 2008; 295(1): G124 - G136.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. Ndiaye, D. H. Poole, and J. L. Pate
Expression and Regulation of Functional Oxytocin Receptors in Bovine T Lymphocytes
Biol Reprod, April 1, 2008; 78(4): 786 - 793.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
C Dosiou, A E Hamilton, Y Pang, M T Overgaard, S Tulac, J Dong, P Thomas, and L C Giudice
Expression of membrane progesterone receptors on human T lymphocytes and Jurkat cells and activation of G-proteins by progesterone
J. Endocrinol., January 1, 2008; 196(1): 67 - 77.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
M. Schumacher, R. Guennoun, A. Ghoumari, C. Massaad, F. Robert, M. El-Etr, Y. Akwa, K. Rajkowski, and E.-E. Baulieu
Novel Perspectives for Progesterone in Hormone Replacement Therapy, with Special Reference to the Nervous System
Endocr. Rev., June 1, 2007; 28(4): 387 - 439.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
L. Josefsberg Ben-Yehoshua, A. L. Lewellyn, P. Thomas, and J. L. Maller
The Role of Xenopus Membrane Progesterone Receptor {beta} in Mediating the Effect of Progesterone on Oocyte Maturation
Mol. Endocrinol., March 1, 2007; 21(3): 664 - 673.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
C. Stocco, C. Telleria, and G. Gibori
The Molecular Control of Corpus Luteum Formation, Function, and Regression
Endocr. Rev., February 1, 2007; 28(1): 117 - 149.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. Thomas, Y. Pang, J. Dong, P. Groenen, J. Kelder, J. de Vlieg, Y. Zhu, and C. Tubbs
Steroid and G Protein Binding Characteristics of the Seatrout and Human Progestin Membrane Receptor {alpha} Subtypes and Their Evolutionary Origins
Endocrinology, February 1, 2007; 148(2): 705 - 718.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
M. B. Hapon, A. B Motta, M. Ezquer, M. Bonafede, and G. A Jahn
Hypothyroidism prolongs corpus luteum function in the pregnant rat
Reproduction, January 1, 2007; 133(1): 197 - 205.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
E. E Nilsson, J. Stanfield, and M. K Skinner
Interactions between progesterone and tumor necrosis factor-{alpha} in the regulation of primordial follicle assembly.
Reproduction, December 1, 2006; 132(6): 877 - 886.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
T. Krietsch, M. S. Fernandes, J. Kero, R. Losel, M. Heyens, E. W.-F. Lam, I. Huhtaniemi, J. J. Brosens, and B. Gellersen
Human Homologs of the Putative G Protein-Coupled Membrane Progestin Receptors (mPR{alpha}, {beta}, and {gamma}) Localize to the Endoplasmic Reticulum and Are Not Activated by Progesterone
Mol. Endocrinol., December 1, 2006; 20(12): 3146 - 3164.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. L. Ashley, C. M. Clay, T. A. Farmerie, G. D. Niswender, and T. M. Nett
Cloning and Characterization of an Ovine Intracellular Seven Transmembrane Receptor for Progesterone that Mediates Calcium Mobilization
Endocrinology, September 1, 2006; 147(9): 4151 - 4159.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
R. Hanna, Y. Pang, P. Thomas, and Y. Zhu
Cell-surface expression, progestin binding, and rapid nongenomic signaling of zebrafish membrane progestin receptors {alpha} and {beta} in transfected cells.
J. Endocrinol., August 1, 2006; 190(2): 247 - 260.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. J. Peluso
Multiplicity of Progesterone's Actions and Receptors in the Mammalian Ovary
Biol Reprod, July 1, 2006; 75(1): 2 - 8.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. J. Peluso, A. Pappalardo, R. Losel, and M. Wehling
Progesterone Membrane Receptor Component 1 Expression in the Immature Rat Ovary and Its Role in Mediating Progesterone's Antiapoptotic Action
Endocrinology, June 1, 2006; 147(6): 3133 - 3140.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2005 by The Endocrine Society