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Dipartimento di Scienze biopatologiche veterinarie, Sezione di Fisiologia, Laboratorio di Biotecnologie fisiologiche (C.B., G.G., G.B., M.M., C.M., D.Z.), and Sezione di Anatomia (C.D., P.C.), Università di Perugia, 06126 Perugia, Italy; and Dipartimento di Biologia molecolare, cellulare e animale (A.G., M.Z.), Università di Camerino, 62032 Camerino, Italy
Address all correspondence and requests for reprints to: Dr. Cristiano Boiti, Dipartimento di Scienze biopatologiche veterinarie, Università of Perugia, S. Costanzo 4, 06126 Perugia, Italy. E-mail: cristiano.boiti{at}unipg.it.
The aim of this study was to better understand the role of the endothelin-1 (ET-1) system in the process of controlling the corpora lutea (CL) life span in rabbits. ET-1 (10 µg iv) administration at d 9 and 12 of pseudopregnancy induced a functional luteolysis within 24 h of injection, but it was ineffective at both d 4 and 6. Pretreatments with Bosentan, a dual ETA/ETB receptor antagonist, or cyclooxygenase (COX) inhibitor blocked the luteolytic action of ET-1 but not that induced by prostaglandin F2
(PGF2). In CL cultured in vitro, ET-1 increased (P 
0.01) both PGF2 production and luteal nitric oxide synthase activity but decreased (P 0.01) progesterone release. Addition of ETA receptor antagonist BQ123 or COX inhibitor blocked the ET-1 luteolytic effects. Positive staining for ET-1 receptors was localized in ovarian blood vessels, granulosa cells of large follicles, and luteal cells. Immunoblot analysis of ET-1 receptor protein revealed a strong band of approximately 48 kDa in d-9 CL. Up to d 6 of pseudopregnancy, ET-1 mRNA abundance in CL was poorly expressed but then increased (P 0.01) at d 9 and 13. ETA-receptor transcript increased (P 0.01) at d 6, remained at the same level up to d 13, and then declined to the lowest (P 0.01) levels at d 22. ETB-receptor mRNA increased (P 0.01) throughout the late-luteal stage from d 13 up to d 18. Our data suggest that the luteolytic action of ET-1 may be a result of PGF2 synthesis from both luteal and accessory cells, via the COX pathways.
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  M. D. Doerr, M. P. Goravanahally, J. D. Rhinehart, E. K. Inskeep, and J. A. Flores Effects of Endothelin Receptor Type-A and Type-B Antagonists on Prostaglandin F2alpha-Induced Luteolysis of the Sheep Corpus Luteum Biol Reprod, April 1, 2008; 78(4): 688 - 696. [Abstract] [Full Text] [PDF]
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C. Boiti, M. Maranesi, C. Dall'Aglio, L. Pascucci, G. Brecchia, A. Gobbetti, and M. Zerani Vasoactive Peptides in the Luteolytic Process Activated by PGF2alpha in Pseudopregnant Rabbits at Different Luteal Stages Biol Reprod, July 1, 2007; 77(1): 156 - 164. [Abstract] [Full Text] [PDF]
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 M Zerani, C Dall'Aglio, M Maranesi, A Gobbetti, G Brecchia, F Mercati, and C Boiti Intraluteal regulation of prostaglandin F2{alpha}-induced prostaglandin biosynthesis in pseudopregnant rabbits Reproduction, May 1, 2007; 133(5): 1005 - 1016. [Abstract] [Full Text] [PDF]
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A. T Grazul-Bilska, C. Navanukraw, M. L. Johnson, D. A Arnold, L. P Reynolds, and D. A Redmer Expression of endothelial nitric oxide synthase in the ovine ovary throughout the estrous cycle. Reproduction, October 1, 2006; 132(4): 579 - 587. [Abstract] [Full Text] [PDF]
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