This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Linscheid, P. Articles by Müller, B. Search for Related Content PubMed PubMed Citation Articles by Linscheid, P. Articles by Müller, B.

Autocrine/Paracrine Role of Inflammation-Mediated Calcitonin Gene-Related Peptide and Adrenomedullin Expression in Human Adipose Tissue

Departments of Research (P.L., D.S., H.Z., U.K., B.M.) and Endocrinology, Diabetology, and Clinical Nutrition (H.Z., U.K., B.M.), University Hospital, CH-4031 Basel, Switzerland

Address all correspondence and requests for reprints to: Philippe Linscheid, Ph.D., Department of Research, University Hospitals, Hebelstrasse 20, 4031 Basel, Switzerland. E-mail: philippe.linscheid{at}unibas.ch.

Human adipose tissue is a contributor to inflammation- and sepsis-induced elevation of serum procalcitonin (ProCT). Several calcitonin (CT) peptides, including ProCT, CT gene-related peptide (CGRP), and adrenomedullin (ADM) are suspected mediators in human inflammatory diseases. Therefore, we aimed to explore the expression, interactions, and potential roles of adipocyte-derived CT peptide production. Expression of CT peptide-specific transcripts was analyzed by RT-PCR and quantitative real-time PCR in human adipose tissue biopsies and three different inflammation-challenged human adipocyte models. ProCT, CGRP, and ADM secretions were assessed by immunological methods. Adipocyte transcriptional activity, glycerol release, and insulin-mediated glucose transport were studied after exogenous CGRP and ADM exposure. With the exception of amylin, CT peptides were expressed in adipose tissue biopsies from septic patients, inflammation-activated mature explanted adipocytes, and macrophage-activated preadipocyte-derived adipocytes. ProCT and CGRP productions were significantly augmented in IL-1ß and lipopolysaccharide-challenged mesenchymal stem cell-derived adipocytes but not in undifferentiated mesenchymal stem cells. In contrast, ADM expression occurred before and after adipogenic differentiation. Interferon- coadministration inhibited IL-1ß-mediated ProCT and CGRP secretion by 78 and 34%, respectively but augmented IL-1ß-mediated ADM secretion by 50%. Exogenous CGRP and ADM administration induced CT, CGRP I, and CGRP II mRNAs and dose-dependently (10^–10 and 10^–6 M) enhanced glycerol release. In contrast, no CGRP- and ADM-mediated effects were noted on ADM, TNF, and IL-1ß mRNA abundances. In summary, CGRP and ADM are two differentially regulated novel adipose tissue secretion factors exerting autocrine/paracrine roles. Their lipolytic effect (glycerol release) suggests a metabolic role in adipocytes during inflammation.

This article has been cited by other articles:

				A. Lacoma, C. Prat, F. Andreo, and J. Dominguez Biomarkers in the management of COPD Eur. Respir. Rev., June 1, 2009; 18(112): 96 - 104. [Abstract] [Full Text] [PDF]

				D. Stolz, M. Christ-Crain, N. G. Morgenthaler, D. Miedinger, J. Leuppi, C. Muller, R. Bingisser, J. Struck, B. Muller, and M. Tamm Plasma Pro-Adrenomedullin But Not Plasma Pro-Endothelin Predicts Survival in Exacerbations of COPD Chest, August 1, 2008; 134(2): 263 - 272. [Abstract] [Full Text] [PDF]

				M. Christ-Crain, B. Kola, F. Lolli, C. Fekete, D. Seboek, G. Wittmann, D. Feltrin, S. C. Igreja, S. Ajodha, J. Harvey-White, et al. AMP-activated protein kinase mediates glucocorticoid-induced metabolic changes: a novel mechanism in Cushing's syndrome FASEB J, June 1, 2008; 22(6): 1672 - 1683. [Abstract] [Full Text] [PDF]

				A. Silaghi, V. Achard, O. Paulmyer-Lacroix, T. Scridon, V. Tassistro, I. Duncea, K. Clement, A. Dutour, and M. Grino Expression of adrenomedullin in human epicardial adipose tissue: role of coronary status Am J Physiol Endocrinol Metab, November 1, 2007; 293(5): E1443 - E1450. [Abstract] [Full Text] [PDF]

				R. Harmancey and F. Smih Response to Comment on: Harmancey et al. (2007) Adrenomedullin Inhibits Adipogenesis Under Transcriptional Control of Insulin: Diabetes 56:553 563 Diabetes, October 1, 2007; 56(10): e18 - e18. [Full Text] [PDF]

				M. Christ-Crain and B. Muller Biomarkers in respiratory tract infections: diagnostic guides to antibiotic prescription, prognostic markers and mediators Eur. Respir. J., September 1, 2007; 30(3): 556 - 573. [Abstract] [Full Text] [PDF]

				K. Takahashi Comment on: Harmancey et al. (2007) Adrenomedullin Inhibits Adipogenesis Under Transcriptional Control of Insulin: Diabetes 56:553 563 Diabetes, September 1, 2007; 56(9): e15 - e15. [Full Text] [PDF]

				E. Tavares, R. Maldonado, and F. J. Minano N-Procalcitonin: Central Effects on Feeding and Energy Homeostasis in Rats Endocrinology, April 1, 2007; 148(4): 1891 - 1901. [Abstract] [Full Text] [PDF]

				M. S. Desruisseaux, Nagajyothi, M. E. Trujillo, H. B. Tanowitz, and P. E. Scherer Adipocyte, Adipose Tissue, and Infectious Disease Infect. Immun., March 1, 2007; 75(3): 1066 - 1078. [Full Text] [PDF]

				R. Harmancey, J.-M. Senard, P. Rouet, A. Pathak, and F. Smih Adrenomedullin Inhibits Adipogenesis Under Transcriptional Control of Insulin Diabetes, March 1, 2007; 56(3): 553 - 563. [Abstract] [Full Text] [PDF]

				O Paulmyer-Lacroix, R Desbriere, M Poggi, V Achard, M-C Alessi, F Boudouresque, L. Ouafik, V Vuaroqueaux, M Labuhn, A Dutourand, et al. Expression of adrenomedullin in adipose tissue of lean and obese women. Eur. J. Endocrinol., July 1, 2006; 155(1): 177 - 185. [Abstract] [Full Text] [PDF]