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Follicular Cells Acquire Sertoli Cell Characteristics after Oocyte Loss

Laboratoire de Physiologie et Physiopathologie (C.J.G., N.C., S.M.-G., S.M.), Centre National de la Recherche Scientifique-Unité Mixte de Recherche 7079, Université Paris VI, Paris, France; and Laboratoire de Pathologie Hormonale Moléculaire (M.G.F.), Institut National de la Santé et de la Recherche Médicale EA3739, Hopital Debrousse, 69322 Lyon cedex 05, France

Address all correspondence and requests for reprints to: Dr. S. Magre, Laboratoire de Physiologie et Physiopathologie, Centre National de la Recherche Scientifique-Unité Mixte de Recherche 7079, Université Paris VI, 7 Quai Saint Bernard, 75005 Paris, France. E-mail: solange.magre{at}snv.jussieu.fr.

Although it has been suggested that in mammals the loss of female germ cells may induce the masculinization of the ovarian compartment, there has been as yet no conclusive demonstration. To directly address that question, the present study has been designed to determine the fate of follicular cells after oocyte loss. Using -irradiation to selectively deplete oocytes in nongrowing follicles in female rats, we show that follicular cells in oocyte-depleted follicles survive, proliferate, and subsequently acquire morphological characteristics of Sertoli cells: elongated cytoplasm, basal location of the nucleus, and specific Sertoli cell junctions, the ectoplasmic specializations. These Sertoli-like cells express, however, the female-specific marker FOXL2 (Forkhead L2) but not the male sex-specific marker SOX-9 (Sry-type high-mobility-group box transcription factor-9) underlying the maintenance of molecular characteristics of granulosa cells. Before transdifferentiating into Sertoli-like cells, follicular cells of oocyte-depleted follicles initiate the expression of anti-Mullerian hormone and inhibin -subunit that are typically synthesized by granulosa cells from the onset of follicular growth. Experimental modifications of the endocrine balance of the irradiated females show that there is a close relationship between plasma FSH levels and the occurrence of Sertoli-like cells. In addition to providing experimental evidence for the crucial role of the oocyte in granulosa cell phenotype maintenance, these results emphasize that the transdifferentiation of granulosa cells into Sertoli cells occurs in a multistep fashion, requiring the maturation of granulosa cells and depending on the endocrine milieu.

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