This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Manning Fox, J. E. Articles by Wheeler, M. B. Search for Related Content PubMed PubMed Citation Articles by Manning Fox, J. E. Articles by Wheeler, M. B.

Oscillatory Membrane Potential Response to Glucose in Islet ß-Cells: A Comparison of Islet-Cell Electrical Activity in Mouse and Rat

Departments of Physiology (J.E.M.F., A.V.G., M.B.W.) and Medicine (M.B.W.), University of Toronto, Ontario, Canada M5S 1A8; and the Department of Pharmacology and Toxicology (L.S.S.), Virginia Commonwealth University, Richmond, Virginia 23298

Address all correspondence and requests for reprints to: Jocelyn Manning Fox, 3352 Medical Sciences Building, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8. E-mail: j.manningfox{at}utoronto.ca.

In contrast to mouse, rat islet ß-cell membrane potential is reported not to oscillate in response to elevated glucose despite demonstrated oscillations in calcium and insulin secretion. We aim to clarify the electrical activity of rat islet ß-cells and characterize and compare the electrical activity of both - and ß-cells in rat and mouse islets. We recorded electrical activity from - and ß-cells within intact islets from both mouse and rat using the perforated whole-cell patch clamp technique. Fifty-six percent of both mouse and rat ß-cells exhibited an oscillatory response to 11.1 mM glucose. Responses to both 11.1 mM and 2.8 mM glucose were identical in the two species. Rat ß-cells exhibited incremental depolarization in a glucose concentration-dependent manner. We also demonstrated electrical activity in human islets recorded under the same conditions. In both mouse and rat -cells 11 mM glucose caused hyperpolarization of the membrane potential, whereas 2.8 mM glucose produced action potential firing. No species differences were observed in the response of -cells to glucose. This paper is the first to demonstrate and characterize oscillatory membrane potential fluctuations in the presence of elevated glucose in rat islet ß-cells in comparison with mouse. The findings promote the use of rat islets in future electrophysiological studies, enabling consistency between electrophysiological and insulin secretion studies. An inverse response of -cell membrane potential to glucose furthers our understanding of the mechanisms underlying glucose sensitive glucagon secretion.

This article has been cited by other articles:

				Y. Zhang, Y. Liu, J. Qu, A. Hardy, N. Zhang, J. Diao, P. J Strijbos, R. Tsushima, R. B Robinson, H. Y Gaisano, et al. Functional characterization of hyperpolarization-activated cyclic nucleotide-gated channels in rat pancreatic {beta} cells J. Endocrinol., October 1, 2009; 203(1): 45 - 53. [Abstract] [Full Text] [PDF]

				E. Tuduri, L. Marroqui, S. Soriano, A. B. Ropero, T. M. Batista, S. Piquer, M. A. Lopez-Boado, E. M. Carneiro, R. Gomis, A. Nadal, et al. Inhibitory Effects of Leptin on Pancreatic {alpha}-Cell Function Diabetes, July 1, 2009; 58(7): 1616 - 1624. [Abstract] [Full Text] [PDF]

				M. G. Pedersen, A. Corradin, G. M Toffolo, and C. Cobelli A subcellular model of glucose-stimulated pancreatic insulin secretion Phil Trans R Soc A, October 13, 2008; 366(1880): 3525 - 3543. [Abstract] [Full Text] [PDF]

				I. Quesada, E. Tuduri, C. Ripoll, and A. Nadal Physiology of the pancreatic {alpha}-cell and glucagon secretion: role in glucose homeostasis and diabetes J. Endocrinol., October 1, 2008; 199(1): 5 - 19. [Abstract] [Full Text] [PDF]

				N. J. Webster, G. J. Searle, P. P. L. Lam, Y.-C. Huang, M. J. Riedel, G. Harb, H. Y. Gaisano, A. Holt, and P. E. Light Elevation in Intracellular Long-Chain Acyl-Coenzyme A Esters Lead to Reduced {beta}-Cell Excitability via Activation of Adenosine 5'-Triphosphate-Sensitive Potassium Channels Endocrinology, July 1, 2008; 149(7): 3679 - 3687. [Abstract] [Full Text] [PDF]

				A. V. Gyulkhandanyan, H. Lu, S. C. Lee, A. Bhattacharjee, N. Wijesekara, J. E. M. Fox, P. E. MacDonald, F. Chimienti, F. F. Dai, and M. B. Wheeler Investigation of Transport Mechanisms and Regulation of Intracellular Zn2+ in Pancreatic {alpha}-Cells J. Biol. Chem., April 11, 2008; 283(15): 10184 - 10197. [Abstract] [Full Text] [PDF]

				W. El-Kholy, P. E. MacDonald, J. M. Fox, A. Bhattacharjee, T. Xue, X. Gao, Y. Zhang, J. Stieber, R. A. Li, R. G. Tsushima, et al. Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Pancreatic {beta}-Cells Mol. Endocrinol., March 1, 2007; 21(3): 753 - 764. [Abstract] [Full Text] [PDF]