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Department of Internal Medicine III (G.M., F.E., M.E., U.C.H.), University of Cologne, Institute of Pharmacology (S.H.), and Center for Molecular Medicine Cologne (S.H., U.C.H.), University of Cologne, 50937 Cologne, Germany
Address all correspondence and requests for reprints to: Uta C. Hoppe, M.D., Department of Internal Medicine III, University of Cologne, Kerpener Strasse 62, 50937 Cologne, Germany. E-mail: uta.hoppe{at}uni-koeln.de.
In the cardiovascular system, T-type calcium channels play an important role for the intracellular calcium homeostasis and spontaneous pacemaker activity and are involved in the progression of structural heart diseases. Androgens influence the cardiovascular physiology and pathophysiology. However, their effect on native T-type calcium currents (ICa,T) remains unclear. To test the chronic effect of testosterone on the cardiac ICa,T, cultured neonatal rat ventricular cardiomyocytes were treated with testosterone (1 nM-10 µM) for 24–30 h. Current measurements were performed after testosterone washout to exclude any acute testosterone effects. Testosterone (100 nM) pretreatment significantly increased whole-cell ICa,T density from 1.26 ± 0.48 pA/pF (n = 8) to 5.06 ± 1.75 pA/pF (n = 7; P < 0.05) and accelerated beating rate. This was attributed to both increased expression levels of the pore-forming subunits Cav3.1 and Cav3.2 and increased T-type single-channel activity. On single-channel level, the increase of the ensemble average current by testosterone vs. time-matched controls was due to an increased availability (58.1 ± 4.2 vs. 21.5 ± 4.0%, P < 0.01) and open probability (2.78 ± 0.29 vs. 0.85 ± 0.23%, P < 0.01). Cotreatment with the selective testosterone receptor antagonist flutamide (10 µM) prevented these chronic testosterone-induced effects. Conversely, acute application of testosterone (10 µM) decreased T-type single-channel activity in testosterone pretreated cells by reducing the open probability (0.78 ± 0.13 vs. 2.91 ± 0.38%, P < 0.01), availability (23.6 ± 3.3 vs. 57.6 ± 4.5%, P < 0.01), and peak current (–20 ± 4 vs. –58 ± 4 fA, P < 0.01). Flutamide (10 µM) did not abolish the testosterone-induced acute block of T-type calcium channels. Our results indicate that long-term testosterone treatment increases, whereas acute testosterone decreases neonatal rat T-type calcium currents. These effects seem to be mediated by a genomic chronic stimulation and a nongenomic acute inhibitory action.
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