This Article Full Text Full Text (PDF) All Versions of this Article: 147/12/5855    most recent Author Manuscript (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Roth, J. D. Articles by Anderson, C. M. Search for Related Content PubMed PubMed Citation Articles by Roth, J. D. Articles by Anderson, C. M.

Antiobesity Effects of the ß-Cell Hormone Amylin in Diet-Induced Obese Rats: Effects on Food Intake, Body Weight, Composition, Energy Expenditure, and Gene Expression

Amylin Pharmaceuticals, Inc., San Diego, California 92121

Address all correspondence and requests for reprints to: Jonathan D. Roth, Ph.D., Amylin Pharmaceuticals, Inc., San Diego, California 92121. E-mail: jonathan.roth{at}amylin.com.

Effects of amylin and pair feeding (PF) on body weight and metabolic parameters were characterized in diet-induced obesity-prone rats. Peripherally administered rat amylin (300 µg/kg·d, 22d) reduced food intake and slowed weight gain: approximately 10% (P < 0.05), similar to PF. Fat loss was 3-fold greater in amylin-treated rats vs. PF (P < 0.05). Whereas PF decreased lean tissue (P < 0.05 vs. vehicle controls; VEH), amylin did not. During wk 1, amylin and PF reduced 24-h respiratory quotient (mean ± SE, 0.82 ± 0.0, 0.81 ± 0.0, respectively; P < 0.05) similar to VEH (0.84 ± 0.01). Energy expenditure (EE mean ± SE) tended to be reduced by PF (5.67 ± 0.1 kcal/h·kg) and maintained by amylin (5.86 ± 0.1 kcal/h·kg) relative to VEH (5.77 ± 0.0 kcal/h·kg). By wk 3, respiratory quotient no longer differed; however, EE increased with amylin treatment (5.74 ± 0.09 kcal/·kg; P < 0.05) relative to VEH (5.49 ± 0.06) and PF (5.38 ± 0.07 kcal/h·kg). Differences in EE, attributed to differences in lean mass, argued against specific amylin-induced thermogenesis. Weight loss in amylin and pair-fed rats was accompanied by similar increases arcuate neuropeptide Y mRNA (P < 0.05). Amylin treatment, but not PF, increased proopiomelanocortin mRNA levels (P < 0.05 vs. VEH). In a rodent model of obesity, amylin reduced body weight and body fat, with relative preservation of lean tissue, through anorexigenic and specific metabolic effects.

This article has been cited by other articles:

				N. T. Bello, M. H. Kemm, and T. H. Moran Salmon calcitonin reduces food intake through changes in meal sizes in male rhesus monkeys Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2008; 295(1): R76 - R81. [Abstract] [Full Text] [PDF]

				J. D. Roth, B. L. Roland, R. L. Cole, J. L. Trevaskis, C. Weyer, J. E. Koda, C. M. Anderson, D. G. Parkes, and A. D. Baron Leptin responsiveness restored by amylin agonism in diet-induced obesity: Evidence from nonclinical and clinical studies PNAS, May 20, 2008; 105(20): 7257 - 7262. [Abstract] [Full Text] [PDF]

				J. J. Hwang, J. L. Chan, G. Ntali, D. Malkova, and C. S. Mantzoros Leptin Does Not Directly Regulate the Pancreatic Hormones Amylin and Pancreatic Polypeptide: Interventional studies in humans Diabetes Care, May 1, 2008; 31(5): 945 - 951. [Abstract] [Full Text] [PDF]

				J. D. Roth, T. Coffey, C. M. Jodka, H. Maier, J. R. Athanacio, C. M. Mack, C. Weyer, and D. G. Parkes Combination Therapy with Amylin and Peptide YY[3 36] in Obese Rodents: Anorexigenic Synergy and Weight Loss Additivity Endocrinology, December 1, 2007; 148(12): 6054 - 6061. [Abstract] [Full Text] [PDF]

				P. K. Chelikani, A. C. Haver, and R. D. Reidelberger Effects of intermittent intraperitoneal infusion of salmon calcitonin on food intake and adiposity in obese rats Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R1798 - R1808. [Abstract] [Full Text] [PDF]

				C. Mack, J. Wilson, J. Athanacio, J. Reynolds, K. Laugero, S. Guss, C. Vu, J. Roth, and D. Parkes Pharmacological actions of the peptide hormone amylin in the long-term regulation of food intake, food preference, and body weight Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R1855 - R1863. [Abstract] [Full Text] [PDF]

				J. D. Roth, H. Hughes, T. Coffey, H. Maier, J. L. Trevaskis, and C. M. Anderson Effects of prior or concurrent food restriction on amylin-induced changes in body weight and body composition in high-fat-fed female rats Am J Physiol Endocrinol Metab, October 1, 2007; 293(4): E1112 - E1117. [Abstract] [Full Text] [PDF]

				A. D. Strader, H. Shi, R. Ogawa, R. J. Seeley, and O. Reizes The Effects of the Melanocortin Agonist (MT-II) on Subcutaneous and Visceral Adipose Tissue in Rodents J. Pharmacol. Exp. Ther., September 1, 2007; 322(3): 1153 - 1161. [Abstract] [Full Text] [PDF]

				L. Aronne, K. Fujioka, V. Aroda, K. Chen, A. Halseth, N. C. Kesty, C. Burns, C. W. Lush, and C. Weyer Progressive Reduction in Body Weight after Treatment with the Amylin Analog Pramlintide in Obese Subjects: A Phase 2, Randomized, Placebo-Controlled, Dose-Escalation Study J. Clin. Endocrinol. Metab., August 1, 2007; 92(8): 2977 - 2983. [Abstract] [Full Text] [PDF]

				S. R. Smith, J. E. Blundell, C. Burns, C. Ellero, B. E. Schroeder, N. C. Kesty, K. S. Chen, A. E. Halseth, C. W. Lush, and C. Weyer Pramlintide treatment reduces 24-h caloric intake and meal sizes and improves control of eating in obese subjects: a 6-wk translational research study Am J Physiol Endocrinol Metab, August 1, 2007; 293(2): E620 - E627. [Abstract] [Full Text] [PDF]