This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Taylor, M. M. Articles by Samson, W. K. Search for Related Content PubMed PubMed Citation Articles by Taylor, M. M. Articles by Samson, W. K.

Intermedin/Adrenomedullin-2 Inhibits Growth Hormone Release from Cultured, Primary Anterior Pituitary Cells

Saint Louis University, Pharmacological and Physiological Science, St. Louis, Missouri 63104

Address all correspondence and requests for reprints to: Dr. Meghan M. Taylor, Saint Louis University, Pharmacological and Physiological Science, 1402 South Grand Boulevard, St. Louis, Missouri 63104. E-mail: taylormm{at}slu.edu.

Intermedin (IMD), a novel member of the adrenomedullin (AM), calcitonin gene-related peptide (CGRP), amylin (AMY) peptide family, has been reported to act promiscuously at all the known receptors for these peptides. Like AM and CGRP, IMD acts in the circulation to decrease blood pressure and in the brain to inhibit food intake, effects that could be explained by activation of the known CGRP, AM, or AMY receptors. Because AM, CGRP, and AMY have been reported to affect hormone secretion from the anterior pituitary gland, we examined the effects of IMD on GH, ACTH, and prolactin secretion from dispersed anterior pituitary cells harvested from adult male rats. IMD, in log molar concentrations ranging from 1.0 pM to 100 nM, failed to significantly alter basal release of the three hormones. Similarly, IMD failed to significantly alter CRH-stimulated ACTH or TRH-stimulated prolactin secretion in vitro. However, IMD concentration-dependently inhibited GHRH-stimulated GH release from these cell cultures. The effects of IMD, although requiring higher concentrations, were as efficacious as those of somatostatin and, like somatostatin, may be mediated, at least in part, by decreasing cAMP accumulation. These actions of IMD were not shared by other members of the AM-CGRP-AMY family of peptides, suggesting the presence of a novel, unique IMD receptor in the anterior pituitary gland and a potential neuroendocrine action of IMD to interact with the hypothalamic mechanisms controlling growth and metabolism.

This article has been cited by other articles:

				M. Chauhan, U. Yallampalli, Y.-L. Dong, G. D.V. Hankins, and C. Yallampalli Expression of Adrenomedullin 2 (ADM2)/Intermedin (IMD) in Human Placenta: Role in Trophoblast Invasion and Migration Biol Reprod, October 1, 2009; 81(4): 777 - 783. [Abstract] [Full Text] [PDF]

				H. Otsubo, S. Hyodo, H. Hashimoto, M. Kawasaki, H. Suzuki, T. Saito, T. Ohbuchi, T. Yokoyama, H. Fujihara, T. Matsumoto, et al. Centrally administered adrenomedullin 5 activates oxytocin-secreting neurons in the hypothalamus and elevates plasma oxytocin level in rats J. Endocrinol., August 1, 2009; 202(2): 237 - 247. [Abstract] [Full Text] [PDF]

				M. Ogoshi, S. Nobata, and Y. Takei Potent osmoregulatory actions of homologous adrenomedullins administered peripherally and centrally in eels Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2008; 295(6): R2075 - R2083. [Abstract] [Full Text] [PDF]

				W. K. Samson, J. V. Zhang, O. Avsian-Kretchmer, K. Cui, G. L. C. Yosten, C. Klein, R.-M. Lyu, Y. X. Wang, X. Q. Chen, J. Yang, et al. Neuronostatin Encoded by the Somatostatin Gene Regulates Neuronal, Cardiovascular, and Metabolic Functions J. Biol. Chem., November 14, 2008; 283(46): 31949 - 31959. [Abstract] [Full Text] [PDF]

				T. Hirose, K. Totsune, N. Mori, R. Morimoto, M. Hashimoto, Y. Nakashige, H. Metoki, K. Asayama, M. Kikuya, T. Ohkubo, et al. Increased expression of adrenomedullin 2/intermedin in rat hearts with congestive heart failure Eur J Heart Fail, September 1, 2008; 10(9): 840 - 849. [Abstract] [Full Text] [PDF]

				M. T. Rademaker, C. J. Charles, M. G. Nicholls, and A. M. Richards Hemodynamic, Hormonal, and Renal Actions of Adrenomedullin 2 in Experimental Heart Failure Circ Heart Fail, July 1, 2008; 1(2): 134 - 142. [Abstract] [Full Text] [PDF]

				S. Nobata, M. Ogoshi, and Y. Takei Potent cardiovascular actions of homologous adrenomedullins in eels Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1544 - R1553. [Abstract] [Full Text] [PDF]

				W. K. Samson, M. M. White, C. Price, and A. V. Ferguson Obestatin acts in brain to inhibit thirst Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R637 - R643. [Abstract] [Full Text] [PDF]

				F. Dong, M. M. Taylor, W. K. Samson, and J. Ren Intermedin (adrenomedullin-2) enhances cardiac contractile function via a protein kinase C- and protein kinase A-dependent pathway in murine ventricular myocytes J Appl Physiol, September 1, 2006; 101(3): 778 - 784. [Abstract] [Full Text] [PDF]