| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Departments of Community and Environmental Medicine (M.T.-T., U.L.), Medicine (U.L.), and Developmental and Cell Biology (U.L.), University of California Irvine, Irvine, California 92617
Address all correspondence and requests for reprints to: Dr. Ulrike Luderer, Center for Occupational and Environmental Health, University of California Irvine, 5201 California Avenue, Suite 100, Irvine, California 92617. E-mail: uluderer{at}uci.edu.
Oxidative stress and depletion of the antioxidant glutathione (GSH) trigger apoptosis in many systems. Previous work showed that antioxidants prevented apoptosis as effectively as FSH in preovulatory follicles. We aimed to test the hypotheses that follicular reactive oxygen species (ROS) initiate apoptosis and that follicular GSH protects against apoptosis. Preovulatory follicles were isolated from ovaries of immature rats primed with pregnant mare serum gonadotropin. Negative control (0-h) follicles were processed immediately. Others were cultured for 2 to 48 h with 1) medium alone, 2) 75 ng/ml ovine FSH, or 3) FSH plus 100 µM buthionine sulfoximine (BSO), a specific inhibitor of GSH synthesis. Total GSH concentrations declined in follicles cultured without FSH for 48 h, whereas FSH increased GSH levels above those observed at 0 h. BSO suppressed GSH to undetectable levels. Treatment with FSH prevented apoptosis in granulosa cells, measured by terminal dUTP transferase-mediated nick-end-labeling and activated caspase 3 immunohistochemistry. Addition of BSO partially and significantly reversed the antiapoptotic effect of FSH on granulosa cells; supplementation of GSH completely prevented BSO-induced granulosa cell apoptosis. Whole-follicle ROS production, measured as dichlorofluorescein and rhodamine fluorescence using confocal microscopy, was significantly increased by 4 h of culture and increased further thereafter. FSH significantly suppressed ROS production, and the addition of BSO partially overcame this effect of FSH. These findings provide evidence that oxidative stress induces apoptosis in preovulatory follicles and that the antiapoptotic effect of FSH is mediated in part by stimulation of follicular GSH synthesis and suppression of ROS production.
This article has been cited by other articles:
 |
|
 |
|
  Y. D. Hoang, B. N. Nakamura, and U. Luderer Follicle-Stimulating Hormone and Estradiol Interact to Stimulate Glutathione Synthesis in Rat Ovarian Follicles and Granulosa Cells Biol Reprod, October 1, 2009; 81(4): 636 - 646. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kang, J. R. Guo, H. M. Yang, R. J. Zhou, J. X. Liu, S. Z. Li, and C. Y. Dong Differential expression profiling of ovarian genes in prelaying and laying geese Poult. Sci., September 1, 2009; 88(9): 1975 - 1983. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Wallace and W. Fan The pathophysiology of mitochondrial disease as modeled in the mouse Genes & Dev., August 1, 2009; 23(15): 1714 - 1736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ortega-Camarillo, A. Gonzalez-Gonzalez, M. Vergara-Onofre, E. Gonzalez-Padilla, A. Avalos-Rodriguez, M. E Gutierrez-Rodriguez, L. Arriaga-Pizano, M. Cruz, L. A. Baiza-Gutman, and M. Diaz-Flores Changes in the glucose-6-phosphate dehydrogenase activity in granulosa cells during follicular atresia in ewes Reproduction, June 1, 2009; 137(6): 979 - 986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Diamanti-Kandarakis, A. Piouka, S. Livadas, C. Piperi, I. Katsikis, A. G Papavassiliou, and D. Panidis Anti-mullerian hormone is associated with advanced glycosylated end products in lean women with polycystic ovary syndrome Eur. J. Endocrinol., May 1, 2009; 160(5): 847 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Cortes-Wanstreet, E. Giedzinski, C. L. Limoli, and U. Luderer Overexpression of glutamate-cysteine ligase protects human COV434 granulosa tumour cells against oxidative and {gamma}-radiation-induced cell death Mutagenesis, May 1, 2009; 24(3): 211 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. H. Ruder, T. J. Hartman, J. Blumberg, and M. B. Goldman Oxidative stress and antioxidants: exposure and impact on female fertility Hum. Reprod. Update, June 4, 2008; (2008) dmn011v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Fan, K. G. Waymire, N. Narula, P. Li, C. Rocher, P. E. Coskun, M. A. Vannan, J. Narula, G. R. MacGregor, and D. C. Wallace A Mouse Model of Mitochondrial Disease Reveals Germline Selection Against Severe mtDNA Mutations Science, February 15, 2008; 319(5865): 958 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tsai-Turton, B. N Nakamura, and U. Luderer Induction of Apoptosis by 9,10-Dimethyl-1,2-Benzanthracene in Cultured Preovulatory Rat Follicles Is Preceded by a Rise in Reactive Oxygen Species and Is Prevented by Glutathione Biol Reprod, September 1, 2007; 77(3): 442 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tsai-Turton, B. T. Luong, Y. Tan, and U. Luderer Cyclophosphamide-Induced Apoptosis in COV434 Human Granulosa Cells Involves Oxidative Stress and Glutathione Depletion Toxicol. Sci., July 1, 2007; 98(1): 216 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yacobi, A. Tsafriri, and A. Gross Luteinizing Hormone-Induced Caspase Activation in Rat Preovulatory Follicles Is Coupled to Mitochondrial Steroidogenesis Endocrinology, April 1, 2007; 148(4): 1717 - 1726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Agca, J. E Ries, S. J Kolath, J.-H. Kim, L. J Forrester, E. Antoniou, K. M Whitworth, N. Mathialagan, G. K Springer, R. S Prather, et al. Luteinization of porcine preovulatory follicles leads to systematic changes in follicular gene expression Reproduction, July 1, 2006; 132(1): 133 - 145. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
