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The Loss of the Chloride Channel, ClC-5, Delays Apical Iodide Efflux and Induces a Euthyroid Goiter in the Mouse Thyroid Gland

Cell Biology Unit (M.-F.v.d.H., K.C.-B., P.J.C.), Christian de Duve Institute of Cellular Pathology, and Nephrology Unit (F.J., O.D.), Université catholique de Louvain, B-1200 Brussels, Belgium; and The Johns Hopkins University School of Medicine (S.E.G., W.B.G.), Baltimore, Maryland 21205

Address all correspondence and requests for reprints to: M.-F. van den Hove, M.D., Cell Unit-ICP/UCL 7541, 75 av Hippocrate, B-1200 Brussels, Belgium. E-mail: vandenhove{at}cell.ucl.ac.be.

Genetic inactivation of ClC-5, a voltage-gated chloride channel prominently expressed in the kidney, leads to proteinuria because of defective apical endocytosis in proximal tubular cells. Because thyroid hormone secretion depends on apical endocytosis of thyroglobulin (Tg), we investigated whether ClC-5 is expressed in the thyroid and affects its function, using Clcn5-deficient knockout (KO) mice. We found that ClC-5 is highly expressed in wild-type mouse thyroid (40% of mRNA kidney level). The protein was immunolocalized at the apical pole of thyrocytes. In Percoll gradients, ClC-5 overlapped with plasma membrane and early endosome markers, but best codistributed with the late endosomal marker, Rab7. ClC-5 KO mice were euthyroid (normal T₄ and TSH serum levels) but developed a goiter with parallel iodine and Tg accumulation (i.e. normal Tg iodination level). When comparing ClC-5 KO with wild-type mice, thyroid ¹²⁵I uptake after 1 h was doubled, incorporation into Tg was decreased by approximately 2-fold, so that trichloroacetic acid-soluble ¹²⁵I increased approximately 4-fold. Enhanced ¹²⁵I^– efflux upon perchlorate and presence of ¹²⁵I-Tg as autoradiographic rings at follicle periphery demonstrated delayed iodide organification. Endocytic trafficking of ¹²⁵I-Tg toward lysosomes was not inhibited. Expression of pendrin, an I^–/Cl^– exchanger involved in apical iodide efflux, was selectively decreased by 60% in KO mice at mRNA and protein levels. Thus, ClC-5 is well expressed in the thyroid but is not critical for apical endocytosis, contrary to the kidney. Instead, the goiter associated with ClC-5 KO results from impaired rate of apical iodide efflux by down-regulation of pendrin expression.

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