This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kamangar, B. B. Articles by Bobe, J. Search for Related Content PubMed PubMed Citation Articles by Kamangar, B. B. Articles by Bobe, J.

Insulin-Like Growth Factor-Binding Protein (IGFBP)-1, -2, -3, -4, -5, and -6 and IGFBP-Related Protein 1 during Rainbow Trout Postvitellogenesis and Oocyte Maturation: Molecular Characterization, Expression Profiles, and Hormonal Regulation

Institute National de la Recherche Agronomique, SCRIBE, Institut Fédératif de Recherche 140, Campus Beaulieu (B.B.K., J.-C.G., J.B.), 35000 Rennes, France; and Department of Fisheries Sciences, Faculty of Agriculture and Natural Resources, University of Kurdestan (B.B.K.), 416 Sanandaj, Iran

Address all correspondence and requests for reprints to: Dr. Julien Bobe, Institute National de la Recherche Agronimique, SCRIBE, Institut Fédératif de Recherche 140, Campus Beaulieu 35000 Rennes, France. E-mail: julien.bobe{at}rennes.inra.fr.

In the present study we report the full coding sequence of rainbow trout IGF-binding protein-1 (IGFBP1), -2, -3, -5, and -6 and IGFBP-related protein-1 (IGFBP-rP1) mRNAs as well as the partial coding sequence of IGFBP-4 mRNA. To our knowledge, this is the first report of IGFBP4, IGFBP6, and IGFBP-rP1 in a nonmammalian species. The tissue distribution of all mRNAs was studied, and the ovarian expression profiles of IGFBP2 to -6 and IGFBP-rP1 between late vitellogenesis and oocyte maturation were characterized. In addition, in vitro hormonal regulation by the maturation-inducing steroid 17,20ß-dihydroxy-4-pregnen-3-one (17,20ßP), gonadotropin, and estradiol were studied. We observed that besides IGFBP1, which was only found in liver, IGFBP2 to -6 and IGFPB-rP1 were expressed in the preovulatory ovary. IGFBP3 was also detected in liver, trunk, kidney, skin, and gills, whereas IGFBP2 to -6 and IGFBP-rP1 exhibited a wider tissue distribution. In the preovulatory ovary, IGFBP3 was strongly down-regulated during the postvitellogenesis period, whereas IGFBP5 exhibited a limited up-regulation. In addition, IGFBP6 and IGFBP-rP1 were up-regulated during oocyte maturation. Hormonal regulation data indicated that all ovarian IGFBPs and IGFBP-rP1 transcripts are regulated under gonadotropic stimulation at a concentration that induced 100% oocyte maturation. In addition, IGFBP2 to -5 transcripts are regulated by 17,20ßP and estradiol. Together, our observations strongly suggest that during final oocyte maturation, a down-regulation of IGFBP3, -4, and -5 occurs in the oocyte in response to gonadotropic and 17,20ßP (IGFBP3 and -5) stimulation, whereas an up-regulation of IGFBP2 and -6 occurs in follicular layers or extrafollicular tissue in response to gonadotropic stimulation.

This article has been cited by other articles:

				M. Shimizu, K. A. Cooper, W. W. Dickhoff, and B. R. Beckman Postprandial changes in plasma growth hormone, insulin, insulin-like growth factor (IGF)-I, and IGF-binding proteins in coho salmon fasted for varying periods Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2009; 297(2): R352 - R361. [Abstract] [Full Text] [PDF]

				X. Wang, L. Lu, Y. Li, M. Li, C. Chen, Q. Feng, C. Zhang, and C. Duan Molecular and functional characterization of two distinct IGF binding protein-6 genes in zebrafish Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2009; 296(5): R1348 - R1357. [Abstract] [Full Text] [PDF]

				M. Li, Y. Li, L. Lu, X. Wang, Q. Gong, and C. Duan Structural, gene expression, and functional analysis of the fugu (Takifugu rubripes) insulin-like growth factor binding protein-4 gene Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2009; 296(3): R558 - R566. [Abstract] [Full Text] [PDF]

				N. I. Bower, X. Li, R. Taylor, and I. A. Johnston Switching to fast growth: the insulin-like growth factor (IGF) system in skeletal muscle of Atlantic salmon J. Exp. Biol., December 15, 2008; 211(24): 3859 - 3870. [Abstract] [Full Text] [PDF]

				M. E Picha, M. J Turano, C. K Tipsmark, and R. J Borski Regulation of endocrine and paracrine sources of Igfs and Gh receptor during compensatory growth in hybrid striped bass (Morone chrysopsxMorone saxatilis) J. Endocrinol., October 1, 2008; 199(1): 81 - 94. [Abstract] [Full Text] [PDF]

				E. Plagnes-Juan, M. Lansard, I. Seiliez, F. Medale, G. Corraze, S. Kaushik, S. Panserat, and S. Skiba-Cassy Insulin regulates the expression of several metabolism-related genes in the liver and primary hepatocytes of rainbow trout (Oncorhynchus mykiss) J. Exp. Biol., August 1, 2008; 211(15): 2510 - 2518. [Abstract] [Full Text] [PDF]

				J. Bobe, S. Mahe, T. Nguyen, H. Rime, D. Vizziano, A. Fostier, and Y. Guiguen A Novel, Functional, and Highly Divergent Sex Hormone-Binding Globulin that May Participate in the Local Control of Ovarian Functions in Salmonids Endocrinology, June 1, 2008; 149(6): 2980 - 2989. [Abstract] [Full Text] [PDF]

				E. M. Santos, V. L. Workman, G. C. Paull, A. L. Filby, K. J. W. Van Look, P. Kille, and C. R. Tyler Molecular basis of sex and reproductive status in breeding zebrafish Physiol Genomics, July 18, 2007; 30(2): 111 - 122. [Abstract] [Full Text] [PDF]

				J.-C. Gabillard, B. B. Kamangar, and N. Montserrat Coordinated regulation of the GH/IGF system genes during refeeding in rainbow trout (Oncorhynchus mykiss). J. Endocrinol., October 1, 2006; 191(1): 15 - 24. [Abstract] [Full Text] [PDF]