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Estrogen Modulates the Spinal N-Methyl-D-Aspartic Acid-Mediated Pelvic Nerve-to-Urethra Reflex Plasticity in Rats

Department of Physiology (S.-Y.L., J.-M.L., H.-Y.P., Y.-Che.H., Y.-Chu.H., T.-B.L.), Graduated Institute of Medical Research (S.-Y.L.), College of Medicine, Department of Obstetrics and Gynecology (G.-D.C.), and Department of Physical Medicine and Rehabilitation (J.-J.L.), Chung-Shan Medical University, Taichung 40201, Taiwan; Department of Biotechnology (S.-F.P.), Ming-Chuan University, Taoyuan 333, Taiwan; Department of Applied Cosmetic (M.-J.C.), Ching-Kuo Institute of Management and Health, Keelong 20346, Taiwan; Department of Anatomy and Cell Biology (J.C.C.), College of Medicine, National Taiwan University, Taipei 106, Taiwan; and School of Medicine (P.-C.H.), Kaohsiung Medical University, Kaohsiung 807, Taiwan

Address all correspondence and requests for reprints to: Dr. Tzer-Bin Lin, Department of Physiology, College of Medicine, Chung-Shan Medical University, No. 110, Chang-Kuo North Road, First Section, Taichung, Taiwan 40201. E-mail: tblin{at}csmu.edu.tw.

The effects of gonadal steroids on glutamate-mediated pelvic nerve-to-urethra reflex (PUR) plasticity were investigated in rats, which received a sham operation (Sham), ovariectomy (OVX), or ovariectomy with daily supplemental estrogen (50 µg/kg, OVX + E₂). The magnitude of the repetitive stimulation (RS, 1 Hz)-induced potentiation in PUR activity decreased significantly in the OVX group when compared with the Sham groups (18.09 ± 3.91 and 7.40 ± 1.03 spikes/stimulation in Sham and OVX group; respectively, P < 0.01, n = 21). Supplemental estrogen (OVX + E₂, 12.60 ± 1.49 spikes/stimulation) significantly reversed the decrease in RS-induced PUR potentiation caused by OVX (P < 0.01, n = 21). The magnitude of the RS-induced potentiation in PUR activity decreased significantly after intrathecal 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo (F) quinoxaline [(20 µM, 10 µl), from 18.09 ± 3.91 to 10.40 ± 0.81, from 7.40 ± 1.03 to 3.20 ± 0.94, and from 12.60 ± 1.49 to 8.06 ± 0.32 spikes/stimulation in Sham, OVX, and OVX + E₂, respectively, P < 0.01, n = 18] and _D-2-amino-5-phosphonoraleric acid [(100 µM, 10 µl), from 18.09 ± 3.91 to 1.04 ± 0.12, from 7.40 ± 1.03 to 1.06 ± 0.22, and from 12.60 ± 1.49 to 0.98 ± 0.25 spikes/stimulation in Sham, OVX, and OVX + E₂, respectively, P < 0.01, n = 18]. In addition, potentiation in PUR activities was induced by intrathecal L-glutamate (0.1 mM, 10 µl, from 1.04 ± 0.02 to 21.60 ± 0.93, from 1.10 ± 0.06 to 8.40 ± 1.50, and from 1.03 ± 0.03 to 18.04 ± 0.84 spikes/stimulation in Sham, OVX, and OVX + E₂, respectively, P < 0.01, n = 18) and N-methyl-D-aspartic acid (0.1 mM, 10 µl, from 1.04 ± 0.02 to 14.80 ± 0.97, from 1.10 ± 0.06 to 4.60 ± 0.48, and from 1.03 ± 0.03 to 9.09 ± 0.63 spikes/stimulation in Sham, OVX, and OVX + E₂); N-methyl-D-aspartic acid-mediated PUR plasticity in female rats and may contribute to alterations in urinary dysfunction after menopause.

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