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Phosphatidylinositol 3-Kinase and Glycogen Synthase Kinase 3 Regulate Estrogen Receptor-Mediated Transcription in Neuronal Cells

Instituto Cajal, Consejo Superior de Investigaciones Cientificas, E-28002 Madrid, Spain

Address all correspondence and requests for reprints to: Dr. L. M. Garcia-Segura, Instituto Cajal, Avenida Doctor Arce 37, E-28002 Madrid, Spain. E-mail: lmgs{at}cajal.csic.es.

In addition to 17ß-estradiol binding, estrogen receptor (ER) transcriptional activity could be controlled by intracellular kinase signaling pathways activated by growth factors. In this report we present evidence suggesting that glycogen synthase kinase 3 (GSK3), an effector kinase of the phosphatidylinositol 3-kinase (PI3K) pathway, may affect ER activity in N2a neuroblastoma cells. LiCl, sodium valproate, and SB415286, three inhibitors of GSK3, dose-dependently blocked ER-mediated transcription. In contrast, overexpression of wild-type GSK3, but not of a mutant inactive form, increased ER-dependent gene expression. Pharmacological or genetic inhibition of the PI3K/Akt pathway, whose activity is inversely correlated with that of GSK3, increased ER-mediated transcription, and this effect was blocked by GSK3 inhibitors. As in other cell types, IGF-I increased ER activity in absence of estradiol by a mechanism independent of PI3K. In contrast, IGF-I decreased ER activity in the presence of estradiol, and this effect was mediated by PI3K. We also observed a regulated interaction between ß-catenin, one of the main GSK3 nuclear targets, and ER. Transfection with a nondegradable mutant of ß-catenin blocked the increase in ER transcriptional activity induced by the PI3K inhibitor wortmannin, suggesting a role for ß-catenin in estrogen signaling. In addition, we investigated the regulation of ER protein levels as a potential mechanism for its regulation by the PI3K/GSK3 pathway; GSK3 blockade increased ER protein stability, whereas PI3K inhibition decreased it. In summary, our findings suggest that ER-dependent gene expression in N2a cells is controlled by the PI3K/Akt/GSK3 signaling pathway.

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