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A Functional Serine 118 Phosphorylation Site in Estrogen Receptor- Is Required for Down-Regulation of Gene Expression by 17ß-Estradiol and 4-Hydroxytamoxifen

Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801

Address all correspondence and requests for reprints to: David J. Shapiro, Department of Biochemistry, University of Illinois, 413 RAL, Box B4, 600 South Mathews, Urbana, Illinois 61801. E-mail: djshapir{at}uiuc.edu.

To evaluate the contribution of ERK1/2 phosphorylation of estrogen receptor (ER)- to activation and repression of endogenous genes, we produced stably transfected lines of HeLa cells with functional ERK1/2 pathways that express similar levels of wild-type human ER and ER mutated to inactivate the well-known MAPK site at serine 118 (ERS118A). We compared effects of the S118A mutation on 17ß-estradiol (E₂)-mediated transactivation, which is heavily dependent on activation function (AF) 2 of ER and on 4-hydroxytamoxifen (OHT)-mediated transactivation, which is heavily dependent on AF1, which includes S118. To examine whether S118 was the key ERK/MAPK phosphorylation site in ER action, we compared the effects of the S118A mutant and the ERK inhibitor U0126 on expression of endogenous genes. In several estrogen response element-containing genes, the S118A mutation strongly reduced induction by E₂, and U0126 did not further reduce expression. Expression of another group of estrogen response element-containing genes was largely unaffected by the S118A mutation. The S118A mutation had variable effects on genes induced by ER tethering or binding near specificity protein-1 and activator protein-1 sites. For five mRNAs whose expression is strongly down-regulated by E₂ and partially or completely down-regulated by OHT, the S118A mutation reduced or abolished down-regulation by E₂ and nearly abolished down-regulation by OHT. In contrast, for Sma and mothers against decapentaplegic-3-related, which is down-regulated by E₂ and not OHT, the S118A mutation had little effect. These data suggest that there may be distinct groups of genes down-regulated by ER and suggest a novel role for ERK phosphorylation at serine 118 in AF1 in regulating expression of the set of genes down-regulated by OHT.

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