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CD8⁺CD122⁺ T Cells, a Newly Identified Regulatory T Subset, Negatively Regulate Graves’ Hyperthyroidism in a Murine Model

Department of Medical Gene Technology, Atomic Bomb Disease Institute (O.S., Y.N.), Division of Immunology, Endocrinology and Metabolism (N.A.), and Division of Clinical Pharmaceutics (M.N.), Department of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan

Address all correspondence and requests for reprints to: Yuji Nagayama, M.D., Department of Medical Gene Technology, Atomic Bomb Disease Institute, Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. E-mail: nagayama{at}nagasaki-u.ac.jp.

Graves’ disease is a thyroid-specific autoimmune disease mediated by stimulatory autoantibodies against the TSH receptor (TSHR). We have previously shown in our mouse model with adenovirus expressing the TSHR that antibody mediated depletion of CD4⁺CD25⁺ regulatory T cells (Tregs) enhances incidence and severity of hyperthyroidism in resistant and susceptible mouse strains, respectively. These data indicate that balance between effector T cells and Tregs is critical for disease development. This study was designed to evaluate the role played by another recently identified type of Treg, CD8⁺CD122⁺ T cells, in our mouse model to delineate the significance of different types of Tregs in Graves’ disease. Flow cytometry analysis showed that CD4⁺CD25⁺ and CD8⁺CD122⁺ T cells are distinct cell types, and anti-CD122 antibody effectively and selectively depleted CD8⁺CD122⁺ T cells. As for CD4⁺CD25⁺ Treg, CD8⁺CD122⁺ T cell depletion increased the incidence of hyperthyroidism both in resistant and susceptible mice. Of interest, intrathyroidal lymphocytic infiltration was observed in some CD8⁺CD122⁺ T cell-depleted, hyperthyroid resistant mice. These results indicate that in addition to CD4⁺CD25⁺ T cells, CD8⁺CD122⁺ T cells also play a crucial role in disease susceptibility in mouse Graves’ disease. Thus, different types of Tregs appear to be involved in tolerance to a self-antigen, the TSHR.

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